Bioavailability of the antihypertensive peptide LHLPLP: Transepithelial flux of HLPLP

Quirós, A. Rodrı́guez-Bernaldo de; Dávalos, Alberto; Lasunción, Miguel A.; Ramos, Mercedes; Recio, Isidra

doi:10.1016/j.idairyj.2007.09.006

Cited by 144 publications

(115 citation statements)

References 51 publications

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“…However, this peptide was hydrolysed to the pentapeptide His-Leu-Pro-Leu-Pro by cellular peptidases before transportation across the intestinal epithelium. The study concluded by use of a Caco-2 monolayer model that the likely mechanism of transport was via paracellular passive diffusion [74]. An earlier study quantifying ACE-inhibitory peptides in human plasma found the pentapeptide to be present in human plasma after oral administration which demonstrates the ability of the peptide to be absorbed through the human brush border membrane [75].…”

Section: Peptide Bioavailabilitymentioning

confidence: 97%

Antihypertensive Peptides from Food Proteins

Norris¹,

Fitzgerald²

2013

Bioactive Food Peptides in Health and Disease

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Section: Peptide Bioavailabilitymentioning

confidence: 97%

Antihypertensive Peptides from Food Proteins

Norris¹,

Fitzgerald²

2013

Bioactive Food Peptides in Health and Disease

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“…Monolayers are deemed intact when TEER values were above 200 Ω cm −2 , and the permeability coeffi cients of the paracellular transport marker fl uorescein is less than 1 × 10 −6 cm s −1 . Apical and basolateral solutions can be collected and analyzed by RP-HPLC-MS/MS to evaluate the permeability, including permeability coefficients as indicated by Quirós et al ( 2008 ) and Contreras et al ( 2012 ), and degradation of tested compounds.…”

Section: Evaluation Of Transepithelial Absorption By Transwell ® Insertsmentioning

confidence: 99%

HT29 Cell Line

Martínez-Maqueda

Miralles

Recio

2015

The Impact of Food Bioactives on Health

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102

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The human colon adenocarcinoma cell line HT29, is not only used to study the biology of human colon cancers, but it is receiving special interest in studies focused on food digestion and bioavailability due to the ability to express characteristics of mature intestinal cells. In the differentiated phenotype, they are able to form a monolayer with tight junctions between cells and a typical apical brush border. In addition, these differentiated cells express brush-border-associated hydrolases typical of the small intestine although the enzymatic activity is lower than that found in vivo. Although they represent a valuable model due to their similarities with enterocytes of the small intestine, their limitations and the relevance to the in vivo situation are also considered in this chapter. The application of this cell line to transport studies of drugs and food compounds is illustrated, especially when the effect of the mucus layer is considered or used as co-culture in combination with Caco-2 cells. They have also been frequently used to study the intestinal immune response to bacterial infection, and microorganism survival, adhesion or invasion. Finally, the use of these cells to evaluate the effect of several food compounds and mucin secretion is summarized. Keywords HT29 • Cell differentiation • Transport studies • Intestinal cell OriginThe human colon adenocarcinoma cell line HT29 was isolated from a primary tumor of a 44 years old Caucasian female in 1964 by Fogh and Trempe ( 1975 ). Since then, many cell lines have been derived from human colon cancers. Initially, this cell line was used to study different aspects of the biology of human cancers. However, these cells have attracted attention due to the fact they were able to express characteristics of mature intestinal cells, such as enterocytes or mucus producing cells.

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“…While their roles in vivo have scarcely been investigated, all these peptides would also be precursors for a potent ACEi peptide of residues 125-129 (HLPLP) (22). A Caco-2 cell study has demonstrated that a longer peptide (residues 124-129) is hydrolyzed to residues 125-129 in the course of transport across the intestinal epithelium, and the residues 125-129 exhibited high resistance to proteolysis in human plasma (34). Despite the limitation in our knowledge of their mechanism of action, milk protein-derived ACEi peptides as well as their precursors may be involved in the modulation of cardiovascular disease later in life (11).…”

Section: β-Cn-derived Bioactive Peptidesmentioning

confidence: 99%