Bioavailability of the flavonol quercetin in cows after intraruminal application of quercetin aglycone and rutin

Berger, L.M.; Wein, Silvia; Blank, Ralf; Metges, C. C.; Wolffram, Siegfried

doi:10.3168/jds.2012-5439

Cited by 68 publications

(97 citation statements)

References 37 publications

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“…Olthof et al [15] , 2000 Human Pure compound Isoquercitin -37 ± 12 1110 ± 48 Chang et al [17] , 2005 Rat Pure compound Isoquercitin -10 -Lesser et al [18] , 2006 Pig Standard diet Quercitin (standard) ± 30 95 -Quercitin (high fat content) 65 Lan et al [19] , 2007 Rat Pure compound Quercitin -114-294 -Krogholm et al [20] , 2010 Human "Juice mix" Quercitin -216 ± 96 -Berger et al [21] , 2012 Cow Standard diet Quercitin -30.0 ± 0.0 -Gohlke et al [22] , Orfali GC et al . Isoquercitrin in cancer above 25 µg/mL, stimulated cell proliferation and cellular metabolism by increasing mitochondrial activity.…”

Section: First Maximummentioning

confidence: 99%

Review of anticancer mechanisms of isoquercitin

Orfali¹,

Duarte²,

Bonadio³

et al. 2016

WJCO

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This review was based on a literature search of PubMed and Scielo databases using the keywords "quercetin, rutin, isoquercitrin, isoquercitin (IQ), quercetin-3-glucoside, bioavailability, flavonols and favonoids, and cancer" and combinations of all the words. We collected relevant scientific publications from 1990 to 2015 about the absorption, bioavailability, chemoprevention activity, and treatment effects as well as the underlying anticancer mechanisms of isoquercitin. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. The subclass of flavonols receives special attention owing to their health benefits. The main components of this class are quercetin, rutin, and IQ, which is a flavonoid and although mostly found as a glycoside, is an aglycone (lacks a glycoside side chain). This compound presents similar therapeutic profiles to quercetin but with superior bioavailability, resulting in increased efficacy compared to the aglycone form. IQ has therapeutic applications owing to its wide range of pharmacological effects including antioxidant, antiproliferative, anti-inflammatory, anti-hypertensive, and anti-diabetic. The protective effects of IQ in cancer may be due to actions on lipid peroxidation. In addition, the antitumor effect of IQ and its underlying mechanism are related to interactions with Wnt signaling pathway, mixed-lineage protein kinase 3, mitogen-activated protein kinase, apoptotic pathways, as well proinflammatory protein signaling. This review contributed to clarifying the mechanisms of absorption, metabolism, and actions of IQ and isoquercitrin in cancer.

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Section: First Maximummentioning

confidence: 99%

Review of anticancer mechanisms of isoquercitin

Orfali¹,

Duarte²,

Bonadio³

et al. 2016

WJCO

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“…One of spotlight herbal phenolic substances is quercetin, which is found in many edible plants, fruits and vegetables (Erlund, 2004;Formica et al, 1995). The bioavailability studies of quercetin and its metabolites; aglycone and rutin, in cows demonstrated that quercetin can be absorbed via digestive tracts and eventually entered the plasma (Berger et al, 2012;Gohlke et al, 2013;Maciej et al, 2015). Several in vivo studies of quercetin in neonatal calves, periparturient and lactating cows, in the aspects of bovine health and production, have been recently reported (Gohlke et al, 2013;Maciej et al, 2015;Stoldt et al, 2015); however, another point of view remains scarce in bovine innate immunity.…”

Section: Introductionmentioning

confidence: 99%

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Srikok

Nambut

Wongsawan

et al. 2017

American Journal of Animal and Veterinary Sciences

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This study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or 50 µM of Quercetin (QH) or Cytochalasin B (CytB), as phagocytosis inhibitor, prior to monitoring phagocytosis of Escherichia coli by flow cytometry and microscopic examination. Additionally, the expressions of CORO1A, CYBA (gp91 phox ), LAMP1, RAB7A, RAC1 and PAK1 mRNA were analyzed by real-time PCR. In the time-course experiment, treated neutrophils were allowed to co-cultured with live bacteria for 30, 60 and 90 min before measuring gene expressions. The expression levels of IL-1 β and TNF genes in order to demonstrate the antiinflammatory property of quercetin were assessed by conventional RT-PCR. The results of flow cytometry and microscopic examination showed that the percentage of neutrophils performing phagocytosis were significantly higher in QH group in comparison with other groups. We reported here that mRNA expressions of CORO1A, CYBA, LAMP1, RAB7A, RAC1 and PAK1 genes involved in phagocytosis, were significantly up-regulated in QH group. As expected, CytB group had profound down-regulation of genes with one exception in PAK1. From our observations, the expression levels of phagocytic process genes in the QH group were optimum at 60 min and started to decline at 90 min of incubation. The data also indicated that quercetin inhibited inflammation by reducing the expressions of both IL-1β and TNF. In conclusion, the information in our experiments conclude that quercetin has the ability to boost the expression of genes involved in phagocytosis while reduces the expression of proinflammatory genes.

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“…However, quercetin in the form of aglycon is always the main metabolite found in plasma (Berger et al, 2012). Unfortunately, there are no records describing flavonoids' bioavailability in the case of chronic, multiple treatment and only this data could reflect the physiological situation when cows are continuously exposed to bioflavonoids present in pasture.…”

mentioning

confidence: 99%

“…example the absolute bioavailability of flavonols after single intraruminal application amounts to 0.1% and 0.5% for quercetin aglycon and rutin (quercetin glycoside), respectively (Berger et al, 2012). However, quercetin in the form of aglycon is always the main metabolite found in plasma (Berger et al, 2012).…”

mentioning

confidence: 99%

“…Taking into account limited bioavailability of flavonoids in adult cows due to ruminal degradation (Berger et al, 2012) but also their potency to increase abomasal contractility (luteolin and quercetin) if used in low doses, it could be estimated that luteolin and quercetin would have an effect of increasing gastric motility. In a study on motility disorders associated with displaced abomasum (DA) in dairy cows, in left DAs and right DAs, the contractile activity was significantly inhibited during electrical stimulation (Geishauser et al, 1998).…”

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confidence: 99%

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Modification of abomasum contractility by flavonoids present in ruminants diet: in vitro study

Mendel

Chłopecka

Dziekan

et al. 2016

animal

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Flavonoid supplementation is likely to be beneficial in improving rumen fermentation and in reducing the incidence of rumen acidosis and bloat. Flavonoids are also said to increase the metabolic performance during the peripartum period. Ruminants are constantly exposed to flavonoids present in feed. However, it is not clear if these phytochemicals can affect the activity of the gut smooth muscle. Therefore, the aim of the study was to verify the effect of three flavonoids on bovine isolated abomasum smooth muscle. The study was carried out on bovine isolated circular and longitudinal abomasal smooth muscle specimens. All experiments were conducted under isometric conditions. The effect of apigenin, luteolin and quercetin (0.001 to 100 µM) was evaluated on acetylcholine-precontracted preparations. The effect of multiple, but not cumulative, treatment and single treatment with each flavonoid on abomasum strips was compared. Apigenin (0.1 to 100 µM) dose-dependently showed myorelaxation effects. Luteolin and quercetin applied in low doses increased the force of the ACh-evoked reaction. However, if used in high doses in experiments testing a wide range of concentrations, their contractile effect either declined (luteolin) or was replaced by an antispasmodic effect (quercetin). Surprisingly, the reaction induced by flavonoids after repeated exposure to the same phytochemical was not reproducible in experiments testing only single exposure of abomasum strips to the same flavonoid used in a high concentration. Taking into account the physicochemical properties of flavonoids, this data suggests the ability of flavonoids to interfere with cell membranes and, subsequently, to modify their responsiveness. Assuming ruminant supplementation with luteolin or quercetin or their presence in daily pasture, a reduction of the likelihood of abomasum dysmotility should be expected.

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Bioavailability of the flavonol quercetin in cows after intraruminal application of quercetin aglycone and rutin

Cited by 68 publications

References 37 publications

Review of anticancer mechanisms of isoquercitin

Review of anticancer mechanisms of isoquercitin

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Modification of abomasum contractility by flavonoids present in ruminants diet: in vitro study

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