Bioavailability of water-soluble CoQ10 in beagle dogs

Validated HPLC method for the quantitative determination of CoQ10 in dog plasma and its application to a pharmacokinetic study

“…n-Hexane and propanol have been widely used for the extraction of CoQ10 from biological matrixes (Edlund, 1988;Okamoto et al, 1988;Grossi et al, 1992;Yamashita and Yamamoto, 1997;Menke et al, 2000;Tang et al, 2001Tang et al, , 2004Mosca et al, 2002;Lu et al, 2003;Galinier et al, 2004;Hansen et al, 2004;Karpinska et al, 2006;Li et al, 2006Li et al, , 2008Hatanaka et al, 2008;Prosek et al, 2008;Balakrishnan et al, 2009;Paliakov et al, 2009), although extraction with less volatile propanol requires more time-consuming solvent evaporation under an air steam. The efficiency of different mixtures of organic solvents for the extraction of CoQ10 was compared under our experimental conditions.…”

“…1997;Menke et al, 2000;Tang et al, 2001Tang et al, , 2004Mosca et Galinier et al, 2004;Miles et al, 2008), and LC coupled with mass spectrometry (MS) (Hansen et al, 2004;Li et al, 2008;Prosek et al, 2008). HPLC-ECD appears to be most commonly used for the simultaneous determination of CoQ10 and CoQ10H2 because of its high selectivity and sensitivity; however, this methodology is complicated, time-consuming and rather used in the clinical laboratory.…”

“…However, most of the above-mentioned HPLC-UV and LC-MS methods did not describe how to address the interference of endogenous CoQ10. Karpinska et al (2006) and Prosek et al (2008) reported external standard methods to determine endogenous CoQ10 concentration by extracting CoQ10 from plasma with n-hexane. Losses in CoQ10 occurred during sample extraction, which led to higher errors.…”

Improvement of Pharmaceutical Properties of Isoprenoid Compounds through the Formation of Cyclodextrin Pseudorotaxane-Like Supramolecules

“…3) Cyclodextrins (CyDs), cyclic oligosaccharides, are able to form inclusion complexes with hydrophobic drugs, improving their water solubility, stability and bioavailability. 4,5) CyDs can also form inclusion complexes with a number of isoprenoid compounds, such as CoQ10, [6][7][8][9] vitamin A, D, E, K, [10][11][12] β-carotene, 13) lycopene, 14) and teprenone, 15) and then improve their water solubility, stability and bioavailability. Likewise, Uekama and colleagues reported that 2,6-di-O-methyl-β-CyD (DM-β-CyD) or γ-CyD markedly improves solubility and oral bioavailability of CoQ10 in rats, dogs or humans.…”

Direct analysis of carbohydrates in animal plasma by ion chromatography coupled with mass spectrometry and pulsed amperometric detection for use as a non-invasive diagnostic tool

“…To provide adequate functioning of the antioxidant defense it is necessary to know and accommodate the nutritional components needed to manage oxidative stress and to evaluate the blood and tissue level of antioxidant capacity. Many degenerative disorders are caused by a depletion of specific cellular antioxidants such as superoxide dismutase (SOD), catalase, vitamin E, vitamin C, carotenoids, coenzyme Q 10 (CoQ 10 ), bilirubin, ␣-lipoic acid (ALA), and glutathione [1][2][3][4][5]. Lipoic acid has been reported to reduce oxidative stress in healthy adults and diabetic patients [6].…”