Biobanking in amphibian and reptilian conservation and management: opportunities and challenges

Strand, Julie; Thomsen, Helena; Jensen, Julie Borup; Marcussen, Cecilie; Nicolajsen, Trine B.; Skriver, Mathias B.; Søgaard, Isabella M.; Ezaz, Tariq; Purup, Stig; Callesen, Henrik; Pertoldi, Cino

doi:10.1007/s12686-020-01142-y

Cited by 32 publications

(26 citation statements)

References 73 publications

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“…As stated above, specimens for marine scientific research are generally conserved over the long term in >70% ethanol, or in formalin for some taxa. Frozen samples or sub-samples (usually DNA, isotope or microbiological samples) require storage at low temperatures usually within biorepositories or biobanks (e.g., Strand et al, 2020). Other organisms may be stored as dried specimens or as series of slides showing important structures for taxonomic differentiation.…”

Section: General Collection For Marine Scientific Researchmentioning

confidence: 99%

Marine Genetic Resources in Areas Beyond National Jurisdiction: Promoting Marine Scientific Research and Enabling Equitable Benefit Sharing

Rogers¹,

Baco

Escobar-Briones

et al. 2021

Front. Mar. Sci.

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Growing human activity in areas beyond national jurisdiction (ABNJ) is driving increasing impacts on the biodiversity of this vast area of the ocean. As a result, the United Nations General Assembly committed to convening a series of intergovernmental conferences (IGCs) to develop an international legally-binding instrument (ILBI) for the conservation and sustainable use of marine biological diversity of ABNJ [the biodiversity beyond national jurisdiction (BBNJ) agreement] under the United Nations Convention on the Law of the Sea. The BBNJ agreement includes consideration of marine genetic resources (MGR) in ABNJ, including how to share benefits and promote marine scientific research whilst building capacity of developing states in science and technology. Three IGCs have been completed to date with the fourth delayed by the Covid pandemic. This delay has allowed a series of informal dialogues to take place between state parties, which have highlighted a number of areas related to MGR and benefit sharing that require technical guidance from ocean experts. These include: guiding principles on the access and use of MGR from ABNJ; the sharing of knowledge arising from research on MGR in ABNJ; and capacity building and technology transfer for developing states. In this paper, we explain what MGR are, the methods required to collect, study and archive them, including data arising from scientific investigation. We also explore the practical requirements of access by developing countries to scientific cruises, including the sharing of data, as well as participation in research and development on shore whilst promoting rather than hindering marine scientific research. We outline existing infrastructure and shared resources that facilitate access, research, development, and benefit sharing of MGR from ABNJ; and discuss existing gaps. We examine international capacity development and technology transfer schemes that might facilitate or complement non-monetary benefit sharing activities. We end the paper by highlighting what the ILBI can achieve in terms of access, utilization, and benefit sharing of MGR and how we might future-proof the BBNJ Agreement with respect to developments in science and technology.

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Section: General Collection For Marine Scientific Researchmentioning

confidence: 99%

Marine Genetic Resources in Areas Beyond National Jurisdiction: Promoting Marine Scientific Research and Enabling Equitable Benefit Sharing

Rogers¹,

Baco

Escobar-Briones

et al. 2021

Front. Mar. Sci.

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“…Um banco de amostras biológicas ou biobanco é, por definição, uma coleção biológica de qualquer amostra humana, animal, vegetal ou microbiana, associadas com os dados da amostra, gerenciados de acordo com padrões técnico-científicos (Hewitt & Watson, 2013). A colheita de material biológico é o primeiro passo para obtenção, onde grande parte pode ser facilmente obtida por métodos não invasivos ante-mortem ou post-mortem durante rotina do veterinário, entretanto, alguns métodos, como coleta de germoplasma em algumas espécies, nem sempre são facilmente obtidas (Strand et al, 2020).…”

Section: Introductionunclassified

Biobancos de animais selvagens: revisão de literatura

Cruz

Oliveira²,

Salvarani³

2022

RSD

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Um banco de amostras biológicas ou biobanco é, por definição, uma coleção biológica de qualquer amostra humana, animal, vegetal ou microbiana, associadas com os dados da amostra, gerenciados de acordo com padrões técnico-científicos. O objetivo deste artigo foi realizar um estudo retrospectivo acerca dos biobancos de animais selvagens, enfatizando sua importância na conservação de espécies, assim como os procedimentos necessários para sua formação, gerenciamento de dados e desafios. Os biobancos devem ser encarados como uma enorme oportunidade de conservação da vida selvagem, por meio da manutenção de amostras biológicas de DNA, células somáticas, tecidos, sangue, germoplasma e embriões, constituindo uma base de dados científicos fundamental para traçar estratégias de proteção de espécies sob ameaça. Todavia, há a necessidade no aprimoramento dos protocolos para uma gama de amostras, com intuito de minimizar as injúrias provocadas pelos crioprotetores e temperaturas de resfriamento ou congelamento. Os biobancos são ferramentas fundamentais no propósito de salvaguardar e gerenciar um conjunto de dados da fauna selvagem, criando uma rede de informações para o desenvolvimento de estratégias de conservação de espécies, frente aos impasses impostos pelos processos de extinção.

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“…The great crested newt ( Triturus cristatus ) has suffered severe declines mainly due to habitat destruction, habitat degradation, pollution, fragmentation, introduction of fish and lack of pond management [ 1 ]. These changes are observed globally among many amphibian species, and the number of species at risk of extinction continues to grow [ 2 , 3 ]. Therefore, various conservation strategies and efforts have been taken in situ, but due to consistent anthropogenic pressures, ex situ strategies such as biobanking are becoming essential for future species conservation [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cryopreservation provides an optimal technique for long-term storage of genetic materials as the biological, chemical and physical processes are suspended due to the low temperatures [ 4 ]. Cell lines grown from tissue samples taken postmortem or via non-lethal techniques are a supplement to biobanking, which has already proven useful in relation to conservation in other vertebrate species such as the African elephant ( Loxodonta Africana) , the clouded leopard ( Neofelis nebulosi) and the western lowland gorilla ( Gorilla gorilla gorilla) [ 3 , 5 , 6 ]. Stored biological material provides opportunities for species-specific stem cell technologies (induced pluripotent stem cells), collection of gametes, production of artificial gametes, in vitro embryo production and embryo transfer [ 3 , 7 ], but use of amphibian cell lines in conservation strategies is still very limited due to lack of basic knowledge [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cell lines grown from tissue samples taken postmortem or via non-lethal techniques are a supplement to biobanking, which has already proven useful in relation to conservation in other vertebrate species such as the African elephant ( Loxodonta Africana) , the clouded leopard ( Neofelis nebulosi) and the western lowland gorilla ( Gorilla gorilla gorilla) [ 3 , 5 , 6 ]. Stored biological material provides opportunities for species-specific stem cell technologies (induced pluripotent stem cells), collection of gametes, production of artificial gametes, in vitro embryo production and embryo transfer [ 3 , 7 ], but use of amphibian cell lines in conservation strategies is still very limited due to lack of basic knowledge [ 2 ]. Cryopreserved genetic material and especially cell lines provide several opportunities, and therefore it is important to ensure the safeguarding of these valuable resources as long-term storage makes it possible to utilize the samples for techniques that have not yet been invented.…”

Section: Introductionmentioning

confidence: 99%

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Establishing Cell Lines from Fresh or Cryopreserved Tissue from the Great Crested Newt (Triturus cristatus): A Preliminary Protocol

Strand

Callesen

Pertoldi

et al. 2021

Animals

Self Cite

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This study describes a successful protocol for establishing cell lines from the threatened Triturus cristatus in terms of collection, preparing, establishing, cryopreserving, thawing and quality checking. Different parameters such as media, media change, fresh vs. cryopreserved tissue and seeding density were tested to optimize culture conditions for this species. With fresh tissue, no considerable differences in the use of two different media were found, but with cryopreserved tissue, a combination of ITS (insulin/transferrin/selenite) and 2-mercaptoethanol had a positive effect on growth. Real-time measurements on the cell lines were used, for the first time in amphibian cells, to investigate the effect of different treatments such as media change with or without washing. Media change had a positive impact on the cells, whereas the effect was negative when combined with washing. It is concluded that establishment of cell lines is possible from the great crested newt, especially when using fresh tissue, but much more challenging if the tissue has been cryopreserved. Real-time measurement during cell culture is a useful tool to visualize the sensitivity of amphibian cells during different culture treatments.

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Biobanking in amphibian and reptilian conservation and management: opportunities and challenges

Cited by 32 publications

References 73 publications

Marine Genetic Resources in Areas Beyond National Jurisdiction: Promoting Marine Scientific Research and Enabling Equitable Benefit Sharing

Marine Genetic Resources in Areas Beyond National Jurisdiction: Promoting Marine Scientific Research and Enabling Equitable Benefit Sharing

Biobancos de animais selvagens: revisão de literatura

Establishing Cell Lines from Fresh or Cryopreserved Tissue from the Great Crested Newt (Triturus cristatus): A Preliminary Protocol

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