Biobynthesis of Carboxymethylated-bacterial cellulose with coconut-water

Abstract

Using coconut-water as main culture medium, carboxymethyl cellulose -bacterial cellulose (CMC-BC) materials were obtained by Acetobacter xylinum through adding CMC as substitute carbon source in static cultivation. When 0.6% (w/v%) CMC was added into the medium, the yield of dried film reached 10.41g/L compared to 4.73 g/L in the control. The FT-IR spectra of the CMC-BC showed the absorption of a carbonyl group at around 1598cm-1 while that of BC without it. This suggested that CMC was incorporated into the cellulose ribbons. Their properties was determined by X-ray diffraction, Scaning electron microscopy (SEM), thermogravimetric analysis (TGA). The CMC-BCs had lower crystallinity index than BC.Scanning electron microscopy (SEM) images showed that the CMC-BC had higher surface area. And the water holding capacity of the CMC-BC products were enhanced. This suggest CMC changed the micro-fibril aggregation of the pellicle to some degree.