Sustainability of the fisheries sector is nowadays a key issue due to the significant impact that this activity may have on the environment. Besides fishing activity itself, other indirect impacts, like those originated from related activities and services also need to be addressed. For assessing the environmental burden of this sector, the indicator Ecological Footprint (EF) can be used. The application of EF to the fisheries sector is still uncommon and studies of associated activities (like ports) even more. In this work, classical EF methodology was applied in order to evaluate the environmental impact of the fisheries sector, taking as a representative sample the global activity (fishing and transportation) of the Port of Vigo (Spain), one of the biggest fishing ports in the world. A high value of total EF for both port and fishing activities was obtained.However, relative EF is much higher in the case of fishing, due to the low natural productivity associated to fish resources. Most of footprint land-components pressure was on energy-land and sea area, being resources consumption the principal category contributing to EF values in all the evaluated scenarios.