Aim: To produce high laccase activities from the white‐rot fungus Trametes hirsuta in an in‐house air‐lift bioreactor (ALB).
Methods and Results: Trametes hirsuta was grown in a 6‐l ALB. A fed‐batch strategy with glycerol as an addition resulted in maximum laccase activity of 19 400 U l−1, which was the highest reported from the fungus.
Conclusion: The ALB configuration with additional glycerol resulted in high laccase activities.
Significance and Impact of the Study: This study provides useful information on how to produce high concentrations of laccase.