Biocatalysis and biotransformation in Brazil: An overview

Birolli, Willian Garcia; Ferreira, Irlon M.; Alvarenga, Natália; Santos, Darlisson de Alexandria; Matos, Iara Lisboa de; Comasseto, J. V.; Porto, André Luiz Meleiro

doi:10.1016/j.biotechadv.2015.02.001

Cited by 40 publications

(21 citation statements)

References 118 publications

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“…sydowii , was able to catalyse asymmetric bioreduction of 4-methoxyacetophenone [ 37 ], hydroxylation of the natural products (–)-ambrox ® , (–)-sclareol, and (+)-sclareolide [ 38 ]; and transformation of 2-phenylacetonitrile to 2-hydroxyphenylacetic acid [ 39 ]. This strain can also degrade lignin [ 40 ] methyl parathion chlorpyrifos [ 41 , 42 ]–an important organophosphate pesticides, and 1,1-dichloro-2,2-bis-(4-chlorophenyl)ethane (DDD)–a chlorinated aromatic insecticide [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

Salt mine microorganisms used for the biotransformation of chlorolactones

et al. 2018

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The aim of the project was to find new catalysts capable of chlorolactone biotransformation. Three bicyclic chlorolactones with structures possessing one or two methyl groups in their cyclohexane ring were subjected to screening biotransformation using seven bacterial strains and one fungal strain from a salt mine. Three strains of bacteria (Micrococcus luteus Pb10, Micrococcus luteus WSP45, Gordonia alkanivorans Pd25) and one fungal strain (Aspergillus sydowii KGJ10) were able to catalyse hydrolytic dehalogenation of one substrate. The classification of the strains that were effective biocatalysts was confirmed by 16S rDNA analysis. The best result (76%) was obtained using Aspergillus sydowii KGJ10. All strains catalysed hydrolytic dehalogenation without changing the conformation. The equatorial position of the chlorine atom in the substrate turned out to be warrant of the positive result of the biotransformation process.

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Section: Resultsmentioning

confidence: 99%

Salt mine microorganisms used for the biotransformation of chlorolactones

et al. 2018

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“…No Brasil existem vários grupos de pesquisa consolidados na área de biocatálise, entretanto, ainda é necessário um grande avanço na área de engenharia de proteínas, em especial utilizando técnicas de evolução dirigida de enzimas, bem como investimentos e incentivos das agências de fomento apoiando o desenvolvimento de projetos na área de biocatálise e na formação de recursos humanos especializados para atuarem na área quimioenzimática. 93,94…”

Section: Reação De Eliminação De Kempunclassified

Evolução Dirigida De Enzimas: Pequenas Modificações, Melhores Biocatalisadores

Teixeira

Milagre

2020

Quim. Nova

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Recebido em 05/11/2019; aceito em 19/03/2020; publicado na web em 24/04/2020 DIRECTED EVOLUTION OF ENZYMES: SMALL CHANGES, BETTER BIOCATALYSTS. Biocatalysis is now a mature field, both in the laboratory and industrial scale. This approach counts on enzyme high selectivity, biodegradability, elegant control over the outcome of reaction conditions and as a possible solution to address some challenges in Green Chemistry faced by synthetic organic chemists. However, many wild-type ready-to-use enzymes were not designed to accommodate the organic substrates needed by today's demands. Or they do not fit a predefined optimum process condition or even, useful reactions are not accessible because there is no enzyme counterpart for metallo-and organocatalysts. This review will give a brief introduction on the protein engineering tools (directed evolution, rational design, and semi-rational design) and will focus on directed evolution of enzymes, their impact in chemistry with examples from production of commodity chemicals to pharmaceutical intermediates and its potential as a tool to achieve greener chemistry criteria will be discussed.

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“…14 Literature reports that marine-derived organisms are sources of diverse enzymes suitable for biotransformation or biocatalytic reactions of organic compounds. 15 In addition, several marine sponge-derived fungi have been applied in the bioreduction of iodoacetophenones, 16 azido-ketones 17 and isatin. 18 However, to date, there is no report about the use of marine sponge's tissue in biotransformation reactions.…”

Section: Introductionmentioning

confidence: 99%

Biotechnological Properties of Sponges from Northeast Brazil: Cliona varians as a Biocatalyst for Enantioselective Reduction of Carbonyl Compounds

Riatto¹,

Victor²,

Sousa³

et al. 2018

J. Braz. Chem. Soc.

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To research the potential ability of whole marine sponges to act as biocatalysts, this paper describes for the first time the employment of whole Cliona varians sponge in the stereoselective reduction of prochiral α-keto esters and isatin to the corresponding chiral alcohols. The addition of D-fructose, D-glucose or sucrose remarkably increased the conversion ratios and stereoselectivities by this marine sponge. Furthermore, in the presence of D-glucose and D-maltose, the reduction of isatin by C. varians afforded the corresponding 3-hydroxyindolin-2-one with high conversions (85-90%) and good enantioselectivities (60-74%). These results showed that the marine sponge presents great potential to be used as biocatalyst for stereoselective reduction of carbonyl compounds.

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Biocatalysis and biotransformation in Brazil: An overview

Cited by 40 publications

References 118 publications

Salt mine microorganisms used for the biotransformation of chlorolactones

Salt mine microorganisms used for the biotransformation of chlorolactones

Evolução Dirigida De Enzimas: Pequenas Modificações, Melhores Biocatalisadores

Biotechnological Properties of Sponges from Northeast Brazil: Cliona varians as a Biocatalyst for Enantioselective Reduction of Carbonyl Compounds

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