Biocatalytic Asymmetric Reduction of Ketones

Ema, Tadashi; Yagasaki, Hideo; Okita, Nobuyasu; Takeda, Masatoshi; Sakai, Takashi

doi:10.1055/s-2006-942026

Cited by 2 publications

(1 citation statement)

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“…It has different pH and temperature stability, different pI values, inhibitors, cofactors, and different substrate interactions depending on which organism it is found in. Carbonyl reductase is not commercially available, but is found in a variety of organisms, such as animals (25), humans (23), and yeast (26). Specifically, it has been extracted in previous studies from many species, such as human placenta (23), Candida viswanathii (27), from the lung of guinea pig (25), and Saccharomyces cervisiae (28,29).…”

Section: Introductionmentioning

confidence: 99%

Towards the Design of an Acetone Breath Biosensor

Hausmann

Meredith

2013

ECS Trans.

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Acetone is one of the most abundant volatile organic compounds (VOC) in human breath and high acetone levels result in ketoacidosis. Many acetone sensors have been developed; however, many disadvantages are present; therefore, there is a need for a more specific, biologically friendly, and simpler biosensor for breath acetone detection for diabetics. This study outlines a procedure for isolation, purification, and characterization of NADPH-dependant carbonyl reductase enzyme for use in an amperometric acetone sensor. Carbonyl reductase was extracted from Saccharomyces pastorianus yeast cells and purified using column chromatography. Carbonyl reductase activity was evaluated using activity assays and was also studied electrochemically. Furthermore, this study outlines a procedure for a flow injection method used for detecting aqueous acetone in the presence of carbonyl reductase. Lastly, various immobilization techniques were tested in order to improve current response and the selectivity towards acetone.

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Section: Introductionmentioning

confidence: 99%

Towards the Design of an Acetone Breath Biosensor

Hausmann

Meredith

2013

ECS Trans.

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The growing number of hemophilia registries: Quantity vs. quality

Keipert

Hesse

Haschberger

et al. 2015

Clin Pharma and Therapeutics

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Registries for rare diseases provide a tool for obtaining an overview of the clinical situation and can be used to discover points of improvement and to monitor long-term safety. Registries could also become a powerful tool to provide supporting information for marketing authorization. There is an urgent need for a pan-European or global strategy that supports consistent data. Therefore, transparency in data collection, harmonization of the database structures, and the convergence of scientific approaches are required.

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Biocatalytic Asymmetric Reduction of Ketones

Cited by 2 publications

References 0 publications

Towards the Design of an Acetone Breath Biosensor

Towards the Design of an Acetone Breath Biosensor

The growing number of hemophilia registries: Quantity vs. quality

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