Bioceramic and polycationic biopolymer nanocomposite scaffolds for improved wound self-healing and anti-inflammatory properties: an <i>in vitro</i> study

Manjubaashini, N.; Bargavi, P.; Balakumar, S.

doi:10.1039/d3bm00169e

Cited by 9 publications

(9 citation statements)

References 86 publications

(97 reference statements)

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“…The surface properties of PCL-MC and PCL-MMC were evaluated using contact angle measurements (Figure (a–d)) to determine their hydrophobic or hydrophilic nature, which is essential for assessing their potential for biological tissue attachment . PCL-MC exhibited a contact angle of 15.71° ± 1.2°, indicating moderate hydrophilicity.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Upon removal of the 3D cottons from the PBS solution, the pH of the solution was promptly measured using a pH meter. The degradation percentage was determined by calculating the mass loss of the 3D cottons using the provided equation: degradation .25em % = W normali − W normalf W normali × 100 where W i is the initial of the 3D bioactive cotton and W f is the final weight of 3D cotton after degradation assay …”

Section: Methodsmentioning

confidence: 99%

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Advancing Bioactive Material for Mandibular Bone Regeneration: Transformation of Fibrous Mat into 3D Matrix Cotton for Enhanced Shape Retention and Rapid Hemostasis

Thomas,

Rajendran,

Purushothaman

et al. 2024

ACS Biomater. Sci. Eng.

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Transformation of a fibrous mat into a three-dimensional (3D) scaffold opens up abundant innovative prospects in biomedical research, particularly for studying both soft as well as hard tissues. Electrospun nanofibers, which mimic the extracellular matrix have attracted significant attention in various studies. This research focuses on rapidly converting a fibrous mat made of polycaprolactone (PCL)/pluronic F-127 (PF-127) with different percentages of monetite calcium phosphate (MCP) into desirable 3D matrix cotton using a unique gas foaming technology. These matrix cottons possess biomimetic properties and have oriented porous structures. Using this innovative technique, various shapes of 3D matrix cotton, such as squares, hollow tubes, and other customizable forms, were successfully produced. Importantly, these 3D matrix cottons showed a consistent distribution of monetite particles with total porosity ranging from 90% to 98%. The structure of the 3D matrix cotton, its water/blood absorption capacity, the potential for causing non-hemolysis, and rapid hemostatic properties were thoroughly investigated. Additionally, periodontal cells were cultured on the 3D matrix cotton to assess their viability and morphology, revealing promising results. Furthermore, a coculture study involving NIH-3T3 and MG-63 cells on the 3D matrix cotton showed spheroidal formation within 24 h. Notably, in vitro assessments indicated that the matrix cotton containing 15% monetite (PCL-MMC15%) exhibited superior absorbent capabilities, excellent cell viability, and rapid hemostatic characteristics. Subsequently, the effectiveness of PCL-MMC15% in promoting mandibular bone regeneration was evaluated through an in vivo study on rabbits using a mandibular injury model. The results demonstrated that PCL-MMC15% facilitated the resolution of defects in the mandibular region by initiating new bone formation. Therefore, the presented 3D matrix cotton (PCL-MMC15%) shows significant promise for applications in both mandibular bone regeneration and hemostasis.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Advancing Bioactive Material for Mandibular Bone Regeneration: Transformation of Fibrous Mat into 3D Matrix Cotton for Enhanced Shape Retention and Rapid Hemostasis

Thomas,

Rajendran,

Purushothaman

et al. 2024

ACS Biomater. Sci. Eng.

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“…The second approach to administering BG to skin wounds involved formulating them into fibers, scaffolds, and composite materials. ,,− ,,− ,,,− Due to their physical characteristics, manipulation of melt-derived BGs presented challenges in the translation to soft tissue applications. Morphologically tailored porous sol–gel BGs provide a means to overcome these challenges.…”

Section: Discussionmentioning

confidence: 99%

Opportunities for Bioactive Glass in Gastrointestinal Conditions: A Review of Production Methodologies, Morphology, Composition, and Performance

Harrop,

Tupally,

Pandey

et al. 2023

Mol. Pharmaceutics

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Bioactive glasses (BGs) are widely used in orthopedic and dental applications for their ability to stimulate endogenous bone formation and regeneration. BG applications more recently broadened to include soft tissue conditions, based on their ability to stimulate angiogenesis, soft tissue regeneration, and wound healing. Sol−gel synthesis has helped facilitate this expansion, allowing formulators to tailor the morphological characteristics of the BG matrix. The effectiveness of BGs in skin wound healing is viewed as a gateway for their use as both a therapeutic and drug delivery platform in other soft tissue applications, notably gastrointestinal (GI) applications, which form the focus of this review. Recent changes in international guidelines for GI conditions shifted clinical objectives from symptom management to mucosal wound healing. The additional scrutiny of proton pump inhibitor (PPI) safety, increasing burden of disease, and financial costs associated with gastroesophageal reflux disease (GERD), peptic ulcer disease (PUD), and inflammatory bowel disease (IBD) open new clinical possibilities for BG. This narrative literature review intersects materials engineering, formulation science, and clinical practice, setting it apart from prior literature. Broadly, current evidence for BG applications in GI conditions is sparse and under-developed, which this review directly addresses. It explores and synthesizes evidence that supports the potential use of sol− gel-derived BG for the efficacious treatment of soft tissue applications, with specific reference to GI conditions. An overview with comparative analysis of current BG synthesis techniques and associated challenges is presented, and influences of composition, biologically active ions, and morphological characteristics in soft tissue applications are explored. To contextualize this, sol−gelderived BGs are proposed as a dual, tailorable therapeutic and drug delivery platform for upper and lower GI conditions. Future directions for this largely untapped area of translational research are also proposed, based on extant literature.

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“…These defects have no self-healing ability, so reconstructive surgery can offer a treatment by using bioactive materials for the regeneration of bone and mend the defects in the specific circumstances. Any bio-alternative substance should exhibit the following characteristics in addition to cyto-biocompatibility: osteoproduction, osteoconduction, bioactivity, adequate mechanical properties, appropriate degradation rate, and antibacterial impact [3,4].…”

Section: Introductionmentioning

confidence: 99%

Devitrite (Na₂Ca₃Si₆O₁₆) phase dominated nanostructured 45S5 bioactive glass: exploring its structural and biological properties

Elakkiya,

Ashok Raja,

Balakumar

2024

Biomed. Mater.

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This research study is primarily centred around calcination temperature and time influence on phase formation in bioactive glasses (BGs). In the present study, bioactive glass with a nominal composition of 45S5 was synthesized through the sol-gel process. The developed BGs then underwent heat treatment for various sintering durations and temperatures. XRD patterns of the BGs reveals that the sintering process led to the crystallization of both devitrite (Na2Ca3Si6O16) and combeite (Na2Ca2Si3O9) phases. The FESEM study divulges morphological alterations, from sheet-like to rod-like structures to eventually transforming into spherical and sheet-like structures. The surface area and Type-IV mesoporous porosity were validated through BET analysis, highlighting a notable increase in pore volume and mechanical strength at a lower sintering temperature. In vitro apatite formation was carried out in Hank’s balance salt in order to evaluate the bioactivity of the glass. After 7 days of immersion in SBF, XRD patterns and SEM micrographs results showed that formation of hydroxyapatite layer on the surface of the BGs. The bioactive glass compatibility with erythrocytes (red blood cells) was also studied, and the results revealed that there was only a low 2% lysis, showing good hemocompatibility. The drug loading and release behaviour of the BGs was studied in the in vitro analysis. The findings showed a high drug encapsulation effectiveness of up to 90% and continuous drug release from the BGs for 24 hours. The materials biocompatibility was unambiguously confirmed by cytocompatibility and proliferation studies. This study provides compelling evidence for the exceptional efficacy and promise of the distinct 45S5 BGs in advancing the field of regenerative medicine.

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Bioceramic and polycationic biopolymer nanocomposite scaffolds for improved wound self-healing and anti-inflammatory properties: an in vitro study

Abstract: Chitosan–45S5 bioglass nanocomposite scaffolds for enhanced wound healing and preventing inflammatory cytokines.

Cited by 9 publications

References 86 publications

Advancing Bioactive Material for Mandibular Bone Regeneration: Transformation of Fibrous Mat into 3D Matrix Cotton for Enhanced Shape Retention and Rapid Hemostasis

Advancing Bioactive Material for Mandibular Bone Regeneration: Transformation of Fibrous Mat into 3D Matrix Cotton for Enhanced Shape Retention and Rapid Hemostasis

Opportunities for Bioactive Glass in Gastrointestinal Conditions: A Review of Production Methodologies, Morphology, Composition, and Performance

Devitrite (Na₂Ca₃Si₆O₁₆) phase dominated nanostructured 45S5 bioactive glass: exploring its structural and biological properties

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Bioceramic and polycationic biopolymer nanocomposite scaffolds for improved wound self-healing and anti-inflammatory properties: an in vitro study

Abstract: Chitosan–45S5 bioglass nanocomposite scaffolds for enhanced wound healing and preventing inflammatory cytokines.

Cited by 9 publications

References 86 publications

Advancing Bioactive Material for Mandibular Bone Regeneration: Transformation of Fibrous Mat into 3D Matrix Cotton for Enhanced Shape Retention and Rapid Hemostasis

Advancing Bioactive Material for Mandibular Bone Regeneration: Transformation of Fibrous Mat into 3D Matrix Cotton for Enhanced Shape Retention and Rapid Hemostasis

Opportunities for Bioactive Glass in Gastrointestinal Conditions: A Review of Production Methodologies, Morphology, Composition, and Performance

Devitrite (Na2Ca3Si6O16) phase dominated nanostructured 45S5 bioactive glass: exploring its structural and biological properties

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Product

Resources

About

Devitrite (Na₂Ca₃Si₆O₁₆) phase dominated nanostructured 45S5 bioactive glass: exploring its structural and biological properties