Biochanin A alleviates gingival inflammation and alveolar bone loss in rats with experimental periodontitis

Zhang, Shengdan; Niu, Yulong; Yang, Zhuo; Zhang, Yuwei; Guo, Qiang; Yang, Yi; Zhou, Xuedong; Ding, Yi; Liu, Chengcheng

doi:10.3892/etm.2020.9381

Cited by 11 publications

(7 citation statements)

References 48 publications

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“…The results for biochanin A are also interesting. It appears that treatment with biochanin A inhibited inflammation, regulated the unbalanced oxidative stress response and improved alveolar bone loss in periodontitis [ 47 ]. Sin et al [ 48 ] showed that flavonols were more potent inhibitors than flavones/isoflavones.…”

Section: Resultsmentioning

confidence: 99%

Multidirectional Effects of Red Clover (Trifolium pratense L.) in Support of Menopause Therapy

et al. 2023

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Red clover is a raw material of interest primarily due to its isoflavone content. However, other groups of compounds may affect the pleiotropic biological effects of this raw material. It is used to alleviate menopausal symptoms, but the fact that there are many varieties of this plant that can be grown makes it necessary to compare the biological activity and phytochemical composition of this plant. Also of interest are the differences between the leaves and flowers of the plant. The aim of this study was to evaluate the properties of the leaves and flowers of six clover varieties—‘Tenia’, ‘Atlantis’, ‘Milena’, ‘Magellan’, ‘Lemmon’ and ‘Lucrum’—with respect to their ability to inhibit α-glucosidase, lipase, collagenase and antioxidant activity. Therefore, the contents of polyphenols and the four main isoflavones—genistein, daidzein, biochanin and formononetin—were assessed. The study was complemented by testing for permeability through a model membrane system (PAMPA). Principal component analysis (PCA) identified a relationship between activity and the content of active compounds. It was concluded that antioxidant activity, inhibition of glucosidase, collagenase and lipase are not correlated with isoflavone content. A higher content of total polyphenols (TPC) was determined in the flowers of red clover while a higher content of isoflavones was determined in the leaves of almost every variety. The exception is the ‘Lemmon’ variety, characterized by high isoflavone content and high activity in the tests conducted.

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Section: Resultsmentioning

confidence: 99%

Multidirectional Effects of Red Clover (Trifolium pratense L.) in Support of Menopause Therapy

et al. 2023

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“…Finally, all rats ( n = 5 per group) were anesthetized with 6% sevoflurane, followed maintain anesthesia with 4% sevoflurane during the experimental procedures. And blood was drawn by cardiac puncture 35 . After the cardiac puncture, the rats were euthanized by CO 2 inhalation followed by cervical dislocation immediately.…”

Section: Methodsmentioning

confidence: 99%

“…And blood was drawn by cardiac puncture. 35 After the cardiac puncture, the rats were euthanized by CO 2 inhalation followed by cervical dislocation immediately. The maxillary jaws and gingiva were harvested.…”

Section: Ovx-periodontitis Rat Model and Study Designmentioning

confidence: 99%

Soy isoflavones alleviate periodontal destruction in ovariectomized rats

Liu

Zhang

Bai

et al. 2022

J of Periodontal Research

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Objective The purpose of this study was to investigate whether soy isoflavone supplementation is effective in preventing periodontal destruction exacerbated by estrogen deficiency (ED) and its potential mechanism. Background The progression of periodontitis is affected by host factors, such as smoking, diabetes mellitus, and steroid use. Bone loss in periodontitis can be aggravated by ED. Methods A rat model of experimental periodontitis (EP) with ED was established by silk ligature and inoculation with Porphyromonas gingivalis, and some EP rats were subjected to bilateral ovariectomy (OVX). The treatment groups received an intravenous injection of 17‐β‐estradiol (E2B) or soy isoflavones (SI) by gavage. The rats were euthanized, and the maxillary jaws, gingiva, and serum were harvested. Tight junction protein and interleukin (IL)‐17 expression, reactive oxygen species (ROS) level, and periodontal destruction were assessed. In addition, we determined whether grainyhead‐like 2 (GRHL2) is required for enhancing the epithelial barrier by SI in an in vitro P. gingivalis infection model. Results Estrogen deficiency impaired the expression of genes encoding tight junction proteins in the gingiva, increased IL‐17 level, and accelerated alveolar bone resorption. SI treatment alleviated tight junction protein expression, decreased IL‐17 and ROS levels, and prevented the absorption of alveolar bone. Furthermore, GRHL2 expression was significantly induced by SI in human oral keratinocytes‐1 (HOK‐1) cells; GRHL2 knockdown impaired the expression of OCLN and ZO‐1 induced by SI treatment. Conclusion Soy isoflavones alleviates periodontitis in OVX rats, as observed by the increased expression of tight junction proteins, and reduced IL‐17 level and alveolar bone loss. The in vitro studies suggested that the enhancement of oral epithelial barrier by SI treatment was partially dependent on GRHL2.

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“…In rat midbrain neuron-glia cultures, BCA has been reported to be effective not only in reducing lipopolysaccharide (LPS)-induced reductions in dopamine uptake and the number of dopaminergic neurons, but also in inhibiting lipopolysaccharide-induced microglial activation of tumour necrosis factor, nitric oxide and superoxide production by microglia [35]. It can also effectively alleviate gingival inflammation, a common oral disease, in rats by inhibiting TNF-ɑ, IL-1β, ROS and elevating OCN a and Nrf2 levels [36].…”

Section: Anti-inflammatory Functionmentioning

confidence: 99%

Chemical and Biological Properties of Biochanin A and Its Pharmaceutical Applications

Feng

Lai

2023

Pharmaceutics

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Biochanin A (BCA), an isoflavone derived from various plants such as chickpea, red clover and soybean, is attracting increasing attention and is considered to have applications in the development of pharmaceuticals and nutraceuticals due to its anti-inflammatory, anti-oxidant, anti-cancer and neuroprotective properties. To design optimised and targeted BCA formulations, on one hand there is a need for more in-depth studies on the biological functions of BCA. On the other hand, further studies on the chemical conformation, metabolic composition and bioavailability of BCA need to be conducted. This review highlights the various biological functions, extraction methods, metabolism, bioavailability, and application prospects of BCA. It is hoped that this review will provide a basis for understanding the mechanism, safety and toxicity of BCA and implementing the development of BCA formulations.

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Biochanin A alleviates gingival inflammation and alveolar bone loss in rats with experimental periodontitis

Cited by 11 publications

References 48 publications

Multidirectional Effects of Red Clover (Trifolium pratense L.) in Support of Menopause Therapy

Multidirectional Effects of Red Clover (Trifolium pratense L.) in Support of Menopause Therapy

Soy isoflavones alleviate periodontal destruction in ovariectomized rats

Chemical and Biological Properties of Biochanin A and Its Pharmaceutical Applications

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