Biochanin A induction of sulfotransferases in rats

Abstract

Biochanin A (BCA) is a dietary isoflavone present in red clover (Trifoliumn pretense) and many herbal products. BCA has been reported to have chemopreventive actions against various cancers including prostate, breast, colon cancer, and so on. Sulfotransferases are a family of phase II drug-metabolizing enzymes, which are important for xenobiotic detoxification and regulation of biological signaling molecule biological activities. Sulfotransferase gene expressions are regulated by different hormones and xenobiotics. Improper regulation of sulfotransferases leads to improper functions of biological signaling molecules, which in turn can cause cancer or other diseases. BCA inhibits the enzyme activities of the phase I drug-metabolizing enzymes CYP1A1 and CYP1B1 in Chinese hamster ovary cells and induces the phase II drug-metabolizing enzymes UDP-glucuronosyltransferases in human prostate cancer cells. BCA induction of sulfotransferases has not been studied. This investigation evaluates the in vivo regulation of sulfotransferases at protein and mRNA levels in the liver and intestine of Sprague-Dawley rats treated with BCA (0, 2, 10, and 50 mg/kg/day) for 7 days. Our experimental results demonstrate for the first time that chronic BCA treatment can significantly induce the expression of rat sulfotransferase 1A1 (rSULT1A1, AST-IV), sulfotransferase 2A1 (rSULT2A1, STa), and rat estrogen sulfotransferase (rSULT1E1, EST) in rat liver and intestine. Our Western blot results are in good agreement with real-time RT-PCR data, suggesting that BCA induction of sulfotransferases occurs at the transcriptional level.

Biochanin A Protects against Acute Carbon Tetrachloride-Induced Hepatotoxicity in Rats

“…[4][5][6] Furthermore, previous studies have reported that it has antimicrobial and anticancer activities. [7][8][9] These properties, in addition to the fact that BCA inhibits phase I and induces phase II metabolizing enzymes, 10) encouraged us to investigate its effectiveness in preventing acute liver injury in rats.…”

Biological Activity of Biochanin A: A Review

“…Biochanin A can significantly induce sulfotransferases enzyme activities and gene expressions in rat liver and intestines. These results may also provide information on the anticancer activities of Biochanin A (Chen et al, 2010).…”

Interaction of manganese(II) complex with apotransferrin and the apotransferrin enhanced anticancer activities

“…AdpaMn was synthesized as reported [8], Hepes(4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid) buffer (50 mM Hepes, 100 mM NaCl, 5 mM Na 2 CO 3 , pH 7.4) was prepared with double-distilled water. Both apoTf and compounds were dissolved in Hepes buffer.…”

“…Recently, we found that Mn(II) compounds with N-substituted di(picolyl)amine inhibits the proliferation of human glioma cells (U251) and HepG-2 cells in vitro. Moreover, Mn(II) compounds [(Adpa)Mn(Cl)(H 2 O)] (Adpa = bis(2-pyridylmethyl)amino-2-propionic acid) (AdpaMn) (Scheme 1) can target mitochondria and inhibits the proliferation of U251 with low IC 50 (9.5 lM) in vitro [7,8]. A significant challenge for biomedical use is the safety of metal compounds, because non-specific interactions between compounds and human plasma proteins can 1386-1425/$ -see front matter Ó 2012 Elsevier B.V. All rights reserved.…”