Biochemical adaptation of wild and cultivated soybean against toxicity of lead salts

Lavrent’yeva, S. I.; Чернышук, Д. К.; Martinenko, Natalia Vladimirovna; Иваченко, Л. Е.; Arsene, Andreea Letiția; Erċışlı, Sezai; Tsatsakis, Aristidis; Голохваст, К. С.; Nawaz, Muhammad Amjad

doi:10.1016/j.etap.2020.103429

Cited by 5 publications

(1 citation statement)

References 31 publications

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“…In order to identify perspective GmCDPK isoforms, the expression patterns of three pairs of AtCDPK1-related paralogs were analysed in wild and cultivated soybeans after abiotic stress treatment. Wild soybeans are more tolerant to adverse climate conditions [ 34 , 35 , 36 ]; therefore, in breeding, they can be a source of the new elite genes that provide protection against stress [ 37 ]. In the present study, a high level of tolerance to extreme high and low temperatures was observed in wild soybeans from the northernmost area ( Figure 1 and Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

ABA-Dependent Regulation of Calcium-Dependent Protein Kinase Gene GmCDPK5 in Cultivated and Wild Soybeans

Veremeichik

Brodovskaya

Grigorchuk

et al. 2022

Life

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Calcium-dependent protein kinases (CDPKs) regulate plant development and stress responses. However, the interaction of these protein kinases with the abscisic acid (ABA) stress hormone signalling system has not been studied in detail. In Arabidopsis, AtCPK1 plays an important role in the acclimation of plants to environmental stresses. Phylogenetic and molecular analyses showed that, among 50 isoforms of Glycine max (L.) Merrill CDPKs, the GmCDPK27/GmCDPK48, GmCDPK5/GmCDPK24, and GmCDPK10/GmCDPK46 paralogous pairs were the isoforms most related to AtCDPK1. We investigated the expression of the corresponding six GmCDPKs genes during treatment with cold, heat, and salt stress. Wild soybean was the most resistant to stresses, and among the three cultivars studied (Sfera, Hodgson, and Hefeng25), Sfera was close to the wild type in terms of resistance. GmCDPK5 and GmCDPK10 were the most responsive to stress treatments, especially in wild soybean, compared with cultivars. Among the studied GmCDPK isoforms, only GmCDPK5 expression increased after treatment with abscisic acid (ABA) in a dose- and time-dependent manner. Targeted LC-MS/MS analysis of endogenous ABA levels showed that wild soybean and Sfera had nearly twice the ABA content of Hodgson and Hefeng25. An analysis of the expression of marker genes involved in ABA biosynthesis showed that GmNCED1-gene-encoding 9-cis-epoxycarotenoid dioxygenase 1 is induced to the greatest extent in wild soybean and Sfera under salt, cold, and heat exposure. Our data established a correlation between the induction of GmCDPK5 and ABA biosynthesis genes. GmCDPK5 is an interesting target for genetic and bioengineering purposes and can be used for genetic editing, overexpression, or as a marker gene in soybean varieties growing under unfavourable conditions.

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