Biochemical Analysis Leads to Improved Orthogonal Bioluminescent Tools

Williams, Sierra J.; Gewing-Mullins, Jordan A; Lieberman, Whitney K.; Kolbaba-Kartchner, Bethany; Iqbal, Reema; Burgess, Hana M; Colee, Clair M; Ornelas, Marya Y.; Reid-McLaughlin, Edison S; Mills, Jeremy H.; Prescher, Jennifer A.; Leconte, Aaron M.

doi:10.1002/cbic.202200726

Cited by 1 publication

(1 citation statement)

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“…In future research, there are several strategies for collaborative design of engineered bacteria and capsules that can expand the dimension of bacteria-to-capsule signals. For example, it is envisioned that by introducing an orthogonal luciferase-luciferin tools 30 , capsules can release luciferin on demand and sequentially detect multiple signals from bacteria. Alternatively, orthogonal quorum sensing genes 31 can be designed for engineered bacteria as output signals, applying bacteria-encapsulating capsules design multiple engineered bacterial sensor channels that respond to each quorum sensing molecules.…”

Section: Discussionmentioning

confidence: 99%

Establishing remote communication between human operator and engineered intestinal microbe via bio-electronic optical language

Zhang,

Feng,

et al. 2024

Preprint

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Synthetic biology enables microbes to be engineered to detect biomarkers and deliver therapies in situ, offering advantages such as self-replication and localized response and treatment. However, the engineered microbes in the intestine remains a "black box", the status of microbial functionality is inaccessible, and the activation of microbial functions is uncontrollable. There is a demand of a "language" between microbes and human for real-time and in-situ monitoring and controlling engineered microbes in the intestine. Light, due to its advantages of specificity and accuracy in the body, was chosen as the language to establish an interactive communication path between human and the microbes with the features of sophisticated monitoring and controlling. For effectively remote operation of this "language" in the intestine, we introduced an electronic capsule as the intermedia, translating information and instructions understandable for human operator into bacterial regulatory and detectable signals in vivo. In this paper, the electronic capsule and the engineered Escherichia coli Nissle 1917 (EcN) strains were designed in a collaborative manner. An EcN-to-capsule bioluminescent signal transmission was established and testified as the language to report the status of engineered EcN, especially the response of EcN to intestinal environment. Similarly, another capsule-to-EcN optogenetic signal transmission was established and testified as the language to control the function of engineered EcN, especially the secretion of therapeutic substances. On this basis, bidirectional communication between human operator and intestinal microbes was achieved via bidirectional bio-electronic optical language. The communication was established to monitor and regulate engineered EcN community in the intestine in 50-90 kg live pigs. The in vitro and in vivo estimations demonstrated the capabilities of monitoring and regulating the engineered microbes, offering a remotely controllable language-based communication between human operator and internal intestinal microbes.

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Section: Discussionmentioning

confidence: 99%