Biochemical analysis of eight different Fusarium oxysporum f.sp. lycopersici isolates using SDS PAGE marker

Abstract

Tomato plants are affected by numerous environmental factors, especially biotic stresses such as fungi, bacteria, viruses, nematodes, and insect pests while growing that lead to yield damage. Globally, Fusarium oxysporum f. sp. lycopersici is considered a crucial pathogen that has been documented to cause damage to tomatoes by decreasing the yield in both open fields and the greenhouse. Fusarium wilt is a soil-borne fungal pathogen capable of surviving in the soil and crop debris for a decade and has been known to enter the plant through wounds on roots. SDS-PAGE analysis was done in eight isolates of Fusarium oxysporum f.sp. lycopersici. The results revealed that the studied isolates of Fusarium were clearly distinguished from each other at the studied biochemical level. The dendrogram obtained from the data showed that hierarchical clustering separated the isolates into three groups according to their similarity coefficients, confirming that some protein-based markers were associated. Therefore, biochemical markers associated with highly pathogenic Fusarium isolates offered a promising alternative to morphological markers to decide the appropriate selection of high pathogeneses for many biological applications.