1980
DOI: 10.1084/jem.152.6.1709
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Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.

Abstract: To isolate a stable tumor cell line capable of producing human interleukin 2 (IL-2; formerly referred to as T cell growth factor), 16 human T and B leukemia cell lines were screened for constitutive and mitogen-stimulated IL-2 production. We found that the T cell leukemia line designated Jurkat-FHCRC produced > 200 U/ml of IL-2 activity after a 24-h stimulation with T cell mitogens. Peak mitogen-induced IL-2 activity was found in supernates harvested from 24-h Jurkat-FHCRC cell cultures stimulated with … Show more

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Cited by 411 publications
(162 citation statements)
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“…The absolute requirement of most cloned CTL for exogenous IL-2 (15,20,33) that is produced by T cells (34)(35)(36) argues that a discrete helper T cell is required and that at least one function of this cell is to provide IL-2 as well as possibly other soluble mediators to CTL.…”
Section: Discussionmentioning
confidence: 99%
“…The absolute requirement of most cloned CTL for exogenous IL-2 (15,20,33) that is produced by T cells (34)(35)(36) argues that a discrete helper T cell is required and that at least one function of this cell is to provide IL-2 as well as possibly other soluble mediators to CTL.…”
Section: Discussionmentioning
confidence: 99%
“…The human T lymphocyte cell line, Jurkat (Gillis and Watson, 1980), was used in this study. The cell line was maintained in RPMI 1640 medium (GIBCO Laboratories, Grand Island, NY) (pRPMI) supplemented with 15%o fetal bovine serum (GIBCO) and penicillin-streptomycin (GIBCO) (cRPMI).…”
Section: Materials and Methods Cell Linesmentioning
confidence: 99%
“…4D). These cytokines were readily detected in culture supernatants of stimulated 2B4.11 or 2B4.hCD40 cells, but not Jurkat cells, which have been propagated as an IL-2-producing human leukemia T cell line (24) and which require overexpression of other proteins to induce TNF-␣ (37) and IFN-␄ (38) secretion. Unlike IL-2, CD40 stimulation alone was able to stimulate both production of TNF-␣ ( p Ï­ 0.02 when 6% 2B4.hCD40 cells were present, p Ï­ 0.001 at 100%) and IFN-␄ ( p Ï­ 0.02 when 6% 2B4.hCD40 cells were present, p Ïœ 0.0001 at 100%) in those cells stably expressing CD40.…”
Section: T Cell Cd40 As a Costimulatory Moleculementioning
confidence: 99%