Biochemical and Biological Properties of Interleukin-3

Several antigen-specific T cell lines were found to secrete a lymphokine upon activation by antigen or lectin that was provisionally termed T cell growth factor III (TCGF III) because it induced the proliferation of a CD4+ T cell clone independently from IL2 and IL4. Amino acid sequence analysis (and the functional properties of TCGF III) revealed that TCGF III was identical with a recently identified lymphokine termed P40. TCGF III/P40 was not only produced by long-term cultured T cell lines but also upon stimulation of freshly isolated Mlsa-reactive T cells. In addition, naive CD4+ T cells secreted TCGF III/P40 upon activation by lectin or allo-major histocompatibility complex structures. However, in spite of its growth-promoting activity for a CD4+ T cell clone this lymphokine does not appear to function as a general growth factor for T cells.

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“…CSP2.1 is an IL 2-dependent [lo], DA-1 an IL 3-dependent [11], and 7TD1 an IL 6-dependent cell line [ 121.…”

Section: Cell Lines and Growth Factor Assaysmentioning

confidence: 99%

Tcgfiii/p40 is produced by naive murine cd4+ t cells but is not a general t cell growth factor*

et al. 1989

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“…The origin, maintenance, and properties of the IL-3-dependent and -independent cell lines used in these studies have been described in detail (6,7,20). The purification and iodination of IL-3 and crosslinking with disuccinimidyl suberate were done as described (8).…”

Section: Methodsmentioning

confidence: 99%

“…IL-3 has been shown to have a variety of biological activities on hematopoietic cells, in part due to its ability to stimulate the proliferation of early stem cells as well as cells committed to various lineages (2)(3)(4). IL-3 is also an essential growth factor for a series of cell lines that have been isolated from long-term bone marrow cultures or from retrovirus-induced primary leukemias (5)(6)(7).…”

mentioning

confidence: 99%

Interleukin 3 binds to a 140-kDa phosphotyrosine-containing cell surface protein.

Isfort

Stevens

May

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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Interleukin 3 (IL-3) is a hematopoietic growth factor that supports theproliferation and differentiation ofearly hematopoietic-lineage progenitors. By using growth factordependent cell lines, IL-3 has been shown to Induce the appearaice ofseveral phosphotyrosine-containing proteins, including a 140-kDa cell surface phosphoprotein. Here we demonstrate that the 140-kDa phosphoprotein binds IL-3 and forms a stable complex with an apparent molecular mass of 170 kDa. The possible relationship of the 140-kDa phosphoprotein to the previously described 65-kDa IL-3-binding protein is discussed.

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“…Yet, other types of CSF (ie, interleukin-3, G-CSF, and CSF-1) have also been implicated in myelopoietic regulation [24,28,35]. In addition, there is no evidence of lactoferrin modulation of CSF production from other sources (ie, T-lymphocytes, fibroblasts, endothelial cells) [9,24].…”

Section: Discussionmentioning

confidence: 98%

In vivo study of lactoferrin and murine rebound myelopoiesis

Poppas¹,

Faith²,

Bierman³

1986

American J Hematol

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Lactoferrin has been reported to inhibit the production of colony-stimulating factor (CSF); thus we sought to study its possible effects on myelopoiesis in vivo. The characteristics of rebound myelopoiesis in C57BL mice injected with a sublethal dose of cyclophosphamide (CY) were used to test lactoferrin for any granulopoietic activity. An experimental group received daily injections of 50 micrograms of human lactoferrin beginning 24 hr after CY injections. By measuring the total nucleated cellularity of the femoral marrow, the peripheral blood count, and the incorporation of tritiated thymidine by the marrow in six replicate experiments, no statistically significant difference was noted between the lactoferrin injected groups and the control groups. Neither the route of lactoferrin administration (i.v. or i.p.) nor the sex of the animal altered the myelopoietic recovery. Lactoferrin had no stimulatory or inhibitory effect on murine rebound myelopoiesis in vivo contrary to the reported in vitro results.

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Biochemical and Biological Properties of Interleukin-3

Cited by 6 publications

References 25 publications

Tcgfiii/p40 is produced by naive murine cd4+ t cells but is not a general t cell growth factor*

Tcgfiii/p40 is produced by naive murine cd4+ t cells but is not a general t cell growth factor*

Interleukin 3 binds to a 140-kDa phosphotyrosine-containing cell surface protein.

In vivo study of lactoferrin and murine rebound myelopoiesis

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