Biochemical and endocrine-disrupting effects in Clarias gariepinus exposed to the synthetic pyrethroids, cypermethrin and deltamethrin

Eni, George; Ibor, Oju R.; Bassey, Andem; Oku, E. E.; Chukwuka, Azubuike V.; Adeogun, Aina O.; Arukwe, Augustine

doi:10.1016/j.cbpc.2019.108584

Cited by 28 publications

(9 citation statements)

References 79 publications

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“…In the current investigations together with previous studies, we provided the evidence to unveil that the presence of DLT, known to be a type II pyrethroid, was able to exert stimulatory actions on vastly different types of I Na , including I Na(T) , I Na(L) , I Na(Tail) , and I Na(P) , seen in pituitary tumor (GH 3 ) cells. It is likely, therefore, that the endocrine disrupting potential caused by the existence of DLT or other structurally similar pyrethroids, as demonstrated recently [ 2 , 16 , 20 , 27 , 37 , 38 , 39 , 40 , 41 ], could be highly linked to the excitatory actions on varying types of I Na presented herein, presuming that similar pharmacological or toxicological actions take place in variable types of endocrine or neuroendocrine cells present in vivo [ 2 , 3 , 7 , 11 , 13 , 42 ], although pyrethroids are thought to be around 2250 times more toxic than mammals [ 12 , 13 ].…”

Section: Discussionmentioning

confidence: 89%

Characterization in Potent Modulation on Voltage-Gated Na+ Current Exerted by Deltamethrin, a Pyrethroid Insecticide

Lin

et al. 2022

IJMS

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Deltamethrin (DLT) is a type-II pyrethroid ester insecticide used in agricultural and domestic applications as well as in public health. However, transmembrane ionic channels perturbed by this compound remain largely unclear, although the agent is thought to alter the gating characteristics of voltage-gated Na+ (NaV) channel current. In this study, we reappraised whether and how it and other related compounds can make any further modifications on voltage-gated Na+ current (INa) in pituitary tumor (GH3) cells. Cell exposure to DLT produced a differential and dose-dependent stimulation of peak (transient, INa(T)) or sustained (late, INa(L)) INa; consequently, the EC50 value required for DLT-stimulated INa(T) or INa(L) was determined to be 11.2 or 2.5 μM, respectively. However, neither the fast nor slow component in the inactivation time constant of INa(T) activated by short depolarizing pulse was changed with the DLT presence; conversely, tefluthrin (Tef), a type-I pyrethroid insecticide, can accentuate INa with a slowing in inactivation time course of the current. The INa(L) augmented by DLT was attenuated by further application of either dapagliflozin (Dapa) or amiloride, but not by chlorotoxin. During pulse train (PT) stimulation, with the Tef or DLT presence, the cumulative inhibition of INa(T) became slowed; moreover, following PT stimuli, a large tail current with a slowly recovering process was observed. Alternatively, during rapid depolarizing pulse, the amplitude of INa(L) and tail INa (INa(Tail)) for each depolarizing pulse became progressively increased by adding DLT, not by Tef. The recovery time constant following PT stimulation with continued presence of Tef or DLT was shortened by further addition of Dapa. The voltage-dependent hysteresis (Hys(V)) of persistent INa was differentially augmented by Tef or DLT. Taken together, the magnitude, gating, frequency dependence, as well as Hys(V) behavior of INa exerted by the presence of DLT or Tef might exert a synergistic impact on varying functional activities of excitable cells in culture or in vivo.

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Section: Discussionmentioning

confidence: 89%

Characterization in Potent Modulation on Voltage-Gated Na+ Current Exerted by Deltamethrin, a Pyrethroid Insecticide

Lin

et al. 2022

IJMS

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“…Del exhibits endocrine-disrupting effects in Clarias gariepinus with dysregulated plasma biochemical and steroid hormone responses. 41 The endocrine toxicity of pyrethroid in fish has long been summarized via interference with endogenous hormones and upstream signaling pathways. 42 Del acts on the estrogenic signaling pathways through the ligation of estrogen receptors in zebrafish larvae.…”

Section: Discussionmentioning

confidence: 99%

“…Two studies of other pesticides confirm that pyrethroids can modulate the steroid hormone levels (such as vitellogenin and choriogenin) and gene expression levels through the hypothalamic-pituitary-gonadal (HPG) axis in fish. 41,46 This hints at whether chronic Del exposure affects sex determination, gonadal development, and reproductive performance in fish.…”

Section: Discussionmentioning

confidence: 99%

Effect of chronic deltamethrin exposure on brain transcriptome and metabolome of juvenile crucian carp

Wu,

Gao,

Xie

et al. 2023

Environmental Toxicology

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Deltamethrin (Del), a widely administered pyrethroid insecticide, has been established as a common contaminant of the freshwater environment and detected in many freshwater ecosystems. In this study, we investigated the changes in brain transcriptome and metabolome of crucian carp after exposure to 0.6 μg/L Del for 28 days. Elevated MDA levels and inhibition of SOD activity indicate damage to the antioxidant system. Moreover, a total of 70 differential metabolites (DMs) were identified using the liquid chromatography‐mass spectrometry, including 32 upregulated and 38 downregulated DMs in the Del‐exposed group. The DMs associated with chronic Del exposure were enriched in steroid hormone biosynthesis, fatty acid metabolism, and glycerophospholipid metabolism for prostaglandin G2, 5‐oxoeicosatetraenoic acid, progesterone, androsterone, etiocholanolone, and hydrocortisone. Transcriptomics analysis revealed that chronic Del exposure caused lipid metabolism disorder, endocrine disruption, and proinflammatory immune response by upregulating the pla2g4, cox2, log5, ptgis, lcn, and cbr expression. Importantly, the integrative analysis of transcriptomics and metabolomics indicated that the arachidonic acid metabolism pathway and steroid hormone biosynthesis were decisive processes in the brain tissue of crucian carp after Del exposure. Furthermore, Del exposure perturbed the tight junction, HIF‐1 signaling pathway, and thyroid hormone signaling pathway. Overall, transcriptome and metabolome data of our study offer a new insight to assess the risk of chronic Del exposure in fish brains.

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“…The results of the present study indicated oocytes atresia and karyoplasmic clumping. According to Eni et al (2019) , alteration in fecundity by limiting the number of eggs produced may be a result of the presence of atretic oocytes in exposed fish. The results of the present study also indicated the presence of melanomacrophage in fish ovary exposed to 0.3 mg/L 4-NP, which may be one of the defense mechanisms to such estrogenic compound because of the increase of atretic follicles.…”

Section: Discussionmentioning

confidence: 99%

Reproductive Toxicity and Recovery Associated With 4-Non-ylphenol Exposure in Juvenile African Catfish (Clarias garepinus)

et al. 2022

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Although, the effects of 4-non-ylphenol (4-NP) on fish’s reproductive hormones were assessed in several studies using adult models, however, the effect of this endocrine disruptor on immature fish’s reproductive hormones was not addressed commonly. We aimed to study the apoptosis induction, hematotoxicity, reproductive toxicity, and the recovery associated with 4-NP exposure in juvenile African catfish [Clarias garepinus) using some hormones [17β-estradiol (E2), testosterone (T), follicle-stimulating hormone (FSH), and luteinizing hormone (LH)] and gonad histology as biomarkers. The toxic effects of 4-NP have been studied in many animal models, but there is still limited knowledge about the dose-dependent damage caused by 4-NP exposure in juvenile Clarias gariepinus. A healthy juvenile C. gariepinus was categorized into four groups (n = 3/group; three replicates in each group). The first group was the control, and the other three groups were subjected to 4-NP concentrations as 0.1, 0.2, and 0.3 mg/L for 15 days; they were left for a recovery period of another 15 days. The reproductive hormones of C. gariepinus exposed to 4-NP for 15 days exhibited significant variations between the treatment groups and the control (P < 0.05), which were evident in E2 and T-values, whereas FSH, LH, total protein, and lipid peroxidation values showed non-significant differences among all groups at P < 0.05. Such a situation referred to the fact that the 15-day recovery period was insufficient to remove the impacts of 4-NP doses in concern. The trend of dose-dependent increase/decrease was recorded for T, E2, FSH, and LH. The histopathological alterations of 4-NP treated in gonad tissues were recorded in juvenile C. gariepinus, reflecting their sensitivity to 4-NP estrogenic-like effects. Overall, our results investigate that recovery has improved the reproductive toxicity caused by 4-NP in juvenile C. garepinus. Significant variations between the treated groups and the control group (P < 0.05) were evident in hematological parameters except for hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC). The parameters exhibiting significance decreased with such increased doses [red blood cells (RBCs), hematocrit (Hct), and white blood cells (WBCs)]. Similar patterns of significant variations toward the increase or decrease were recorded following the 15-day recovery period. Apoptotic frequency in erythrocytes and brain cells has increased significantly with increased 4-NP exposure, indicating that 4-NP caused cytotoxic effects, such as apoptosis in a dose-dependent manner. However, these cellular alterations greatly decreased after the 15-day recovery period.

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Biochemical and endocrine-disrupting effects in Clarias gariepinus exposed to the synthetic pyrethroids, cypermethrin and deltamethrin

Cited by 28 publications

References 79 publications

Characterization in Potent Modulation on Voltage-Gated Na+ Current Exerted by Deltamethrin, a Pyrethroid Insecticide

Characterization in Potent Modulation on Voltage-Gated Na+ Current Exerted by Deltamethrin, a Pyrethroid Insecticide

Effect of chronic deltamethrin exposure on brain transcriptome and metabolome of juvenile crucian carp

Reproductive Toxicity and Recovery Associated With 4-Non-ylphenol Exposure in Juvenile African Catfish (Clarias garepinus)

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