Biochemical and enzyme inhibitory attributes of methanolic leaf extract of Datura inoxia Mill.

Bagewadi, Zabin K.; Muddapur, Uday M.; Madiwal, Savita S.; Mulla, Sikandar I.; Khan, Aejaz Abdullatif

doi:10.1007/s42398-019-00052-6

Cited by 61 publications

(46 citation statements)

References 36 publications

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“…The ANOVA using the Dunnett Test showed that the antiradical activities of our bark extracts (p-value = 0.004 < 0.05) and leaves (p-value = 0.0001 < 0.05) are all lower than those of the positive controls tested: ascorbic acid with 3.05 ± 0.07 µg/ml, rutin with 2.79 ± 0.79 µg/ml and quercetin with 1.17 ± 0.03 µg/ml (Figure 4.). The high content of polyphenols and flavonoids (Figure 2) in the extracts would be responsible for the anti-free radical activities observed in our extracts; since a direct correlation between antioxidant activity and the reducing power of plant extracts involving phenolic compounds has been reported by many authors (Fatima et al, 2015;Iqbal et al, 2017;Togola et al, 2019;Fofié et al, 2017;Bagewadi et al, 2019). Other secondary metabolites (tannins, terpenes, sterols, etc.…”

Section: Discussionsupporting

confidence: 60%

Evaluation of the biological activities of leaf and bark extracts of Ficus platiphylla Delile, a medicinal plant used in Mali

KonarÃ¹,

Diarra²,

CissÃ³

et al. 2020

J. Med. Plants Res.

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Leaves and barks of Ficus platiphylla are used in traditional medicine in western Africa for treating bacterial infections. The aim of this work was to screen the biological activities of extracts of the plant. Three common bacterial strains were used in this study. The antiradical and antibacterial activities were assessed respectively by spectrophotometric and agar diffusion methods. The bark and leaves are very rich in dry matter, ash and as well as proteins and carbohydrates. Ethyl acetate was the best solvent to extract polyphenols (4.77-5.01 g/100 g) and flavonoids (1.97-3.32 g/100 g). Similarly, ethyl acetate extracts showed the best anti-free radical activity with IC 50 = 5.10 and 5.22 µg/ml for bark and leaves respectively. The aqueous and methanolic leaf extracts displayed the highest antibacterial activity on the Escherichia coli, with an inhibition diameter (ID) of 11.50 ± 0.50 mm. On Salmonella typhi, the most active antibacterial agents were found in leaf ethyl acetate extracts with ID = 15.33 ± 0.58 mm. These extracts have the same efficacy as the commercial antibiotic ciprofloxacin (positive control) which displayed an ID of 15.33 ± 0.76 mm. Finally, S. aureus was highly sensitive to ethyl acetate extracts of the barks: ID = 15.50 ± 1.32 mm (p-value = 4.10E-6 < 0.05). The richness in secondary metabolites associated with the antiradical and antibacterial activities justify use of F. platiphylla in traditional medicine. This study shows that F. platiphylla could be a valuable medicinal plant for the management of common bacterial infections.

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Section: Discussionsupporting

confidence: 60%

Evaluation of the biological activities of leaf and bark extracts of Ficus platiphylla Delile, a medicinal plant used in Mali

KonarÃ¹,

Diarra²,

CissÃ³

et al. 2020

J. Med. Plants Res.

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“…Joshi and Kaur (2013), when investigating D. inoxia leaves collected randomly and aseptically from different areas of India found a greater diversity of secondary metabolites (alkaloids, phenolic compounds, flavonoids, tannins, terpenoids and steroids) in the aqueous extract in relation to the methanolic and ethanolic extracts (alkaloids, phenolic and flavonoid compounds and steroids). Recently, Bagewadi et al (2019) determined that the phytochemical profiling of the methanolic leaf extract of D. inoxia collected in India revealed the occurrence of important phytochemicals, with a predominance of alkaloids in relation to the phenols, carbohydrates, flavonoids, saponins, and terpenoids.…”

Section: Resultsmentioning

confidence: 99%

Phytochemistry and antifungal potential of Datura inoxia Mill. on soil phytopathogen control

Matias

Fernandes²,

Corrêa³

et al. 2020

Biosci. J.

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The application of chemical pesticides for the control of fungal diseases results in impacts on the environment and human health. The use of vegetal extracts with antifungal properties for the proper management of crops becomes a viable alternative, mainly for organic and family farming. The objective of this study was to carry out the phytochemical evaluation of Datura inoxia, evaluating its antifungal potential against the mycelial growth of Fusarium solani and Sclerotinia sclerotiorum. The extracts, aqueous and ethanolic, obtained from the leaves of the plant collected in areas of the municipality of Campo Grande, Mato Grosso do Sul, were submitted to phytochemical prospecting and quantification of flavonoids and total phenols. It was evaluated its antifungal activity at concentrations of 800, 1200, 1600, 2000, and 2400 μg 100 mL-1. Each concentration was separately incorporated into BDA agar, poured into Petri dishes, and inoculated with the mycelial disc of the fungus. The diameter of the colonies were measured daily. Two solutions were prepared as control, one containing the solvent added to PDA medium (ethanol solution), and another with only PDA medium (without D. inoxia extract, control). In both extracts were found the same diversity of secondary metabolites (nine classes). The ethanolic extract, a solvent of lower polarity than water, was more efficient in the extraction of these constituents. Alkaloids and phenolic compounds were the most frequent compounds (100%). In relation to antifungal activity, the ethanolic extract provided 100% inhibition of mycelial growth of Sclerotinia sclerotitorum in all concentrations, relative to the control. On the other hand, the growth of Fusarium solani was only negatively affected at the highest concentrations of 800 and 1200 μmL-1 100 mL-1. The antifungal potential of Datura inoxia was probably related to the abundance of alkaloids and phenolic compounds in its chemical constitution that negatively effects the development of the vegetative mycelium.

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“…In vitro antimicrobial activity of extracted biosurfactant (5.0 mg/ml) was evaluated against Gram-negative bacteria such as E. coli, S. typhimurium and Pseudomonas aeruginosa and Gram-positive bacteria such as M. luteus, S. aureus and B. cereus. Biosurfactant was also tested against yeast C. tropicalis by agar well diffusion method described previously by Bagewadi et al (2019). The antimicrobial activity was assessed on the basis of zone of inhibition (mm).…”

Section: Assessment Of Antimicrobial Properties Of Biosurfactantmentioning

confidence: 99%

Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2

Yaraguppi

Bagewadi

Muddapur

et al. 2020

J Petrol Explor Prod Technol

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A potential biosurfactant producing isolate was identified as Bacillus aryabhattai strain ZDY2. Biosurfactant production was enhanced by 2.51-fold through the development of an optimized process using response surface methodology. The optimized culture medium contained crude oil 4.0%, yeast extract 0.7% and NaNO 3 3.0% that yielded 8.86 g/l of biosurfactant. Biosurfactant was characterized for stability up to 100 °C, at pH 5-10 and in the presence of NaCl concentration up to 8%. Biosurfactant demonstrated antimicrobial activity against Salmonella typhimurium, Escherichia coli, Micrococcus luteus, Staphylococcus aureus and Candida tropicalis. The morphological characterization was carried out by scanning electron microscopy with energy-dispersive X-ray analysis. The Fourier-transform infrared spectroscopy analysis reveals the lipopeptide nature of the biosurfactant produced by B. aryabhattai strain ZDY2. The biosurfactant finds application in healthcare and pharmaceutical industries.

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Biochemical and enzyme inhibitory attributes of methanolic leaf extract of Datura inoxia Mill.

Cited by 61 publications

References 36 publications

Evaluation of the biological activities of leaf and bark extracts of Ficus platiphylla Delile, a medicinal plant used in Mali

Evaluation of the biological activities of leaf and bark extracts of Ficus platiphylla Delile, a medicinal plant used in Mali

Phytochemistry and antifungal potential of Datura inoxia Mill. on soil phytopathogen control

Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2

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