Biochemical and functional properties of a pyruvate formate‐lyase (PFL)‐activating system in <i>Streptococcus mutans</i>

Takahashi-Abbe, S.; K, Abe; Takahashi, Nobuhiro

doi:10.1034/j.1399-302x.2003.00081.x

Cited by 20 publications

(21 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pyruvate formate-lyase (PFL), exclusively existent in the H Group, converts sugar into volatile compounds (formate, acetate and ethanol) and serves in ATP synthesis and NAD+/NADH recycling [38]. This enzyme is extremely sensitive to oxygen and can be key to anaerobic fermentation in dental plaques [39]. N-acetylmuramoyl-L-alanine amidase is an autolytic enzyme bound to the surface of bacterial cell walls.…”

Section: Discussionmentioning

confidence: 99%

Saliva Microbiota Carry Caries-Specific Functional Gene Signatures

et al. 2014

View full text Add to dashboard Cite

Human saliva microbiota is phylogenetically divergent among host individuals yet their roles in health and disease are poorly appreciated. We employed a microbial functional gene microarray, HuMiChip 1.0, to reconstruct the global functional profiles of human saliva microbiota from ten healthy and ten caries-active adults. Saliva microbiota in the pilot population featured a vast diversity of functional genes. No significant distinction in gene number or diversity indices was observed between healthy and caries-active microbiota. However, co-presence network analysis of functional genes revealed that caries-active microbiota was more divergent in non-core genes than healthy microbiota, despite both groups exhibited a similar degree of conservation at their respective core genes. Furthermore, functional gene structure of saliva microbiota could potentially distinguish caries-active patients from healthy hosts. Microbial functions such as Diaminopimelate epimerase, Prephenate dehydrogenase, Pyruvate-formate lyase and N-acetylmuramoyl-L-alanine amidase were significantly linked to caries. Therefore, saliva microbiota carried disease-associated functional signatures, which could be potentially exploited for caries diagnosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Saliva Microbiota Carry Caries-Specific Functional Gene Signatures

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Also, S. mutans has a PFL‐activating system that operates optimally at pH 6.8. This system includes a PFL‐activating enzyme that itself is activated by an electron transport system that transfers an electron from NADH to the PFL‐activating enzyme (Takahashi‐Abbe et al ., ). The resulting rapid acid production in the deep layers of dental plaque likely confers the high cariogenic potential of this microorganism.…”

Section: Central Metabolismmentioning

confidence: 97%

From meadows to milk to mucosa – adaptation ofStreptococcusandLactococcusspecies to their nutritional environments

Zeyniyev

Kuipers

et al. 2012

FEMS Microbiol Rev

View full text Add to dashboard Cite

Lactic acid bacteria (LAB) are indigenous to food-related habitats as well as associated with the mucosal surfaces of animals. The LAB family Streptococcaceae consists of the genera Lactococcus and Streptococcus. Members of the family include the industrially important species Lactococcus lactis, which has a long history safe use in the fermentative food industry, and the disease-causing streptococci Streptococcus pneumoniae and Streptococcus pyogenes. The central metabolic pathways of the Streptococcaceae family have been extensively studied because of their relevance in the industrial use of some species, as well as their influence on virulence of others. Recent developments in high-throughput proteomic and DNA-microarray techniques, in in vivo NMR studies, and importantly in whole-genome sequencing have resulted in new insights into the metabolism of the Streptococcaceae family. The development of cost-effective high-throughput sequencing has resulted in the publication of numerous whole-genome sequences of lactococcal and streptococcal species. Comparative genomic analysis of these closely related but environmentally diverse species provides insight into the evolution of this family of LAB and shows that the relatively small genomes of members of the Streptococcaceae family have been largely shaped by the nutritionally rich environments they inhabit.

show abstract

“…sensitive to oxygen (Takahashi et al, 1987;Takahashi-Abbe et al, 2003). It has been shown in other lactic acid bacteria that the PDH complex facilitates heterofermentation that is accomplished in an oxygen-rich environment.…”

mentioning

confidence: 99%

The involvement of the pyruvate dehydrogenase E1Î± subunit, inStreptococcus mutansacid tolerance

Korithoski¹,

Lévesque²,

Cvitkovitch³

2008

FEMS Microbiology Letters

View full text Add to dashboard Cite

Streptococcus mutans, an etiological agent of dental caries, is a normal inhabitant of dental plaque. Two main virulence factors of S. mutans are acidogenicity and aciduricity - the ability to produce acid and survive at low pH, respectively. Metabolic processes, including the catabolism of pyruvate, are finely regulated following acid exposure in S. mutans. Proteome analysis of the S. mutans acid response has shown pyruvate dehydrogenase A (PdhA) is upregulated. PdhA is the E1alphasubunit of the four-enzyme pyruvate dehydrogenase complex, responsible for the heterofermentative catalysis of pyruvate into acetyl-CoA. Acetyl-CoA is subsequently catalyzed into ethanol and acetate yielding additional ATP. This investigation examined the relationships between PdhA, aciduricity, and metabolism in S. mutans. An S. mutans pdhA knockout (PDHAKO) revealed an acid sensitive phenotype, displayed by increased doubling times, and decreased competitiveness in a biofermentor. Quantitative real-time PCR showed pdhA expression increased dramatically during acidic growth and under acid adaptation. Additionally, pdhA expression responded to conditions favoring heterofermentative growth; decreased in the presence of excess glucose, and increased during stationary phase compared with mid-log phase growth. This study demonstrated that in S. mutans, pdhA expression responds to conditions conducive to heterofermentation and deletion of pdhA resulted in decreased aciduricity.

show abstract

Biochemical and functional properties of a pyruvate formate‐lyase (PFL)‐activating system in Streptococcus mutans

Cited by 20 publications

References 25 publications

Saliva Microbiota Carry Caries-Specific Functional Gene Signatures

Saliva Microbiota Carry Caries-Specific Functional Gene Signatures

From meadows to milk to mucosa – adaptation ofStreptococcusandLactococcusspecies to their nutritional environments

The involvement of the pyruvate dehydrogenase E1Î± subunit, inStreptococcus mutansacid tolerance

Contact Info

Product

Resources

About