2023
DOI: 10.1101/2023.04.25.538249
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Biochemical and genetic dissection of the RNA-binding surface of the FinO domain ofEscherichia coliProQ

Abstract: RNA-binding proteins play important roles in bacterial gene regulation through interactions with both coding and non-coding RNAs. ProQ is a FinO-domain protein that binds a large set of RNAs in Escherichia coli, though the details of how ProQ binds these RNAs remain unclear. In this study, we used a combination of in vivo and in vitro binding assays to confirm key structural features of E. coli ProQ's FinO domain and explore its mechanism of RNA interactions. Using a bacterial three-hybrid assay, we performed … Show more

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