Biochemical and genetic diversity of pectolytic enterobacteria causing soft rot disease of potatoes in New Zealand

Pitman, Andrew R.; Wright, P.J.; Galbraith, Matthew D.; Harrow, Sally A.

doi:10.1071/ap08056

Cited by 44 publications

(48 citation statements)

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“…This is the first report of P. wasabiae in potatoes in South Africa. Previous reports of P. wasabiae strains infecting potatoes were in the USA, New Zealand and Iran (Kim et al, 2009;Pitman et al, 2008;Baghaee-Ravari et al, 2011).…”

Section: Phylogenetic Analysis Of Atypical South African Strainsmentioning

confidence: 99%

“…As a result, new atypical and highly pathogenic Pectobacterium spp. strains have been identified and these are associated with new potato disease outbreaks in different countries (Pitman et al, 2008;van der Merwe et al, 2010;Baghaee-Baghari et al, 2011). For this reason, it is important that new potentially invasive species are identified and characterised to minimise the impact of the SRE on potato productivity worldwide.…”

Section: Introductionmentioning

confidence: 99%

“…brasiliensis (van der Merwe et al, 2010). Pectobacterium wasabiae, originally isolated from horse radish, has been shown to cause diseases in potatoes and ornamental plants in the USA, New Zealand and Iran (Goto and Matsumoto, 1987;Kim et al, 2009;Pitman et al, 2008;BaghaeeRavari et al, 2011). However, there has been no previous report of P. wasabiae in South Africa.…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa

et al. 2012

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Pectolytic bacteria were isolated from potato tubers and stems showing tuber soft rot and blackleg symptoms. Approximately half (52%) of the isolates could grow at both 27 and 37 °C while another half (48%) failed to grow at 37 °C. All isolates could be amplified with primers specific to the pectate lyase (pel) gene. Carbon utilisation profiles could not conclusively identify these isolates. PCR amplification using primers specific for Pectobacterium carotovorum subsp. brasiliensis was positive for all isolates that grew at 37°C. However, the group that did not grow at 37 °C failed to amplify with P. atrosepticum specific primers. To characterise this group of isolates, the intergenic transcribed spacer region (ITS) was amplified and PCR products digested with two restriction enzymes (RsaI and CfoI) to generate ITS-PCR-RFLP profiles. The profiles of these new isolates were compared to those of the type strains of other pectolytic bacteria. Profiles of five of the selected atypical strains generated with the enzyme CfoI appeared to be most similar to those of P. wasabiae type strain. Phylogenetic analysis using concatenated partial gene sequences of housekeeping genes mdh and gapA clustered these isolates together with those of P.wasabiae reference strains thus confirming their identity. These strains were virulent on potato tubers and stems but did not elicit hypersensitive response on tobacco plants. This is the first report of P. wasabiae causing soft rot and blackleg of potatoes in South Africa.

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Section: Phylogenetic Analysis Of Atypical South African Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa

et al. 2012

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“…As Pcc is usually unable to cause blackleg and Pba is only rarely isolated from tubers in New Zealand (Pitman et al, 2008), Pbr is likely to be an important component of the blackleg syndrome in New Zealand. Consequently, this pathogen is expected to contribute to economic losses resulting from both blackleg and tuber soft rot.…”

mentioning

confidence: 99%

“…A collection of 89 enterobacteria were recently isolated from potato tubers from commercial crops in the Auckland, Waikato, Manawatu-Wanganui and Canterbury regions of New Zealand. The majority were initially assigned as Pcc by their growth at 37°C, carbon utilisation profiles and restriction fragment length polymorphisms (Pitman et al, 2008). These isolates were mostly unable to cause blackleg symptoms, although several were shown to be highly aggressive upon stem infection.…”

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confidence: 99%

First report of blackleg and soft rot of potato caused by Pectobacterium carotovorum subsp. brasiliensis in New Zealand

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et al. 2012

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The Potato Crop

2020

154

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Biochemical and genetic diversity of pectolytic enterobacteria causing soft rot disease of potatoes in New Zealand

Cited by 44 publications

References 32 publications

Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa

Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa

First report of blackleg and soft rot of potato caused by Pectobacterium carotovorum subsp. brasiliensis in New Zealand

The Potato Crop

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