Biochemical and Genetical Studies on Variants of the Mouse Strain L Cells in Cell Culture

Biochemical and Genetical Studies on Radioresistant Mouse Cells in Culture

“…These Lr7 cells survived after total 14,000R of irradiation and showed a higher radioresistance than the original L cells. Their resistance to radiation was not so remarkable, however, as the resistance of other variants to chemical and physical agents (Horikawa 1961).…”

“…The original L cells were grown in YLH medium (Horikawa 1961) supplemented with 5 % bovine serum. On the other hand, the original Ehrlich ascites tumor cells were grown in YLH medium supplemented with 20% bovine serum.…”

“…Previously (Horikawa 1961) it was suggested that the appearance of resistant cells, excej t those resistant to ionizing radiation, seemed to depend on adaptation to or selection by the respective agents rather than on mutagenic action. Whitfield and Rixon (1960) derived a cell line resistant to ionizing radiation after a single irradiation.…”

Microculture Method of Peripheral Blood for Chromosome Study in Mice

“…The culture medium was composed of 50% of YLH solution (Horikawa 1961), 25% of calf serum and 25% of bovine serum. 0.2 ml of Phytohemagglutinin-M (Difco) was added to 10 ml of the above medium.…”

Photo‐pattern analysis and computation in the evaluation of the cytotoxicity of dental materials in vitro

“…The method ot nucleus number counting (NC) has been approved as a critical method in the evaluation of cytotoxicity and cell damage (Katsuta et al 1954). The NC method provides similar data to those obtained from measurements ofthe DNA, RNA and protein content per tissue culture bottle over 7-day periods (Horikawa 1961). Many studies on Correspondence: Professor Haruyuki Kawabara, Institute of Clinical Materials, Higasbiyama +-8, I ligasbi-Osaka, Osaka 579, Japan.…”