Biochemical and Histological Studies on Prostates in Castrated Dogs After Treatment With Androstanediol, Oestradiol and Cyproterone Acetate

Tunn, U.; Senge, Th.; Schenck, B.; Neumann, F.

doi:10.1530/acta.0.0910373

Cited by 60 publications

(34 citation statements)

References 12 publications

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“…Long-term treatment of adult rats with E2 causes prostatic involution (9) and ultimately leads to epithelial squamous metaplasia (10). Moreover, there appears to be a dose-dependent variation in the effects of E2.…”

Section: Discussionmentioning

confidence: 99%

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Early effects of estrogen on the rat ventral prostate

García-Flórez

Oliveira

Carvalho

2005

Braz J Med Biol Res

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Complex interactions between androgen and estrogen (E2) regulate prostatic development and physiology. We analyzed the early effects of a high single dose of E2 (25 mg/kg body weight) and castration (separately or combined) on the adult 90-day-old male Wistar rat ventral prostate. Androgen levels, prostate weight, and the variation in the relative and absolute volume of tissue compartments and apoptotic indices were determined for 7 days. Castration and exogenous E2 markedly reduced ventral prostate weight (about 50% of the control), with a significant reduction in the epithelial compartment and increased stroma. The final volume of the epithelium was identical at day 7 for all treatments (58.5% of the control). However, E2 had an immediate effect, causing a reduction in epithelial volume as early as day 1. An increase in smooth muscle cell volume resulted from the concentration of these cells around the regressing epithelium. The treatments resulted in differential kinetics in epithelial cell apoptosis. Castration led to a peak in apoptosis at day 3, with 5% of the epithelial cells presenting signs of apoptosis, whereas E2 caused an immediate increase (observed on day 1) and a sustained (up to day 7) effect. E2 administration to castrated rats significantly increased the level of apoptosis by day 3, reaching 9% of the epithelial cells. The divergent kinetics between treatments resulted in the same levels of epithelial regression after 7 days (~30% of control). These results show that E2 has an immediate and possibly direct effect on the prostate, and anticipates epithelial cell death before reducing testosterone to levels as low as those of castrated rats. In addition, E2 and androgen deprivation apparently cause epithelial cell death by distinct and independent pathways.

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Section: Discussionmentioning

confidence: 99%

“…Long-term treatment of adult rats with E2 causes prostatic involution (9) and ultimately leads to epithelial squamous metaplasia (10). In castrated dogs, E2 (8 mg/dog per week) has a proliferative effect on both stroma and epithelium (11).…”

Section: Introductionmentioning

confidence: 99%

Early effects of estrogen on the rat ventral prostate

García-Flórez

Oliveira

Carvalho

2005

Braz J Med Biol Res

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“…These results are in agreement with several reports indicating that administration of estrogen to castrated or hypophysectomized animals could exert a stimulatory influence on prostate epithelial cells. In castrated or hypophysectomized dogs, estrogen induced hypertrophy of epithelial cells (Leav et al 1978;Tunn et al 1979;Merk et al 1980Merk et al ,1986Kwan et al 1982). Similarly, in castrated rats, estrogens increase epithelial cell height in the ventral prostate (Salander and Tisell 1976;Thompson et al 1979;Timms and Chandler 1985).…”

Section: Discussionmentioning

confidence: 99%

Effects of Estradiol on Prostate Epithelial Cells in the Castrated Rat

Pelletier

2002

J Histochem Cytochem.

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S U M M A R YThere is evidence that estrogens can modulate the activity of prostate epithelial cells. To determine whether estradiol can have a direct influence on rat prostate, this study examined the effects of estradiol-17 ␤ (E 2 ) administered alone or in combination with dihydrotestosterone (DHT) to castrated rats for 3 weeks on prostate binding protein (PBP) C1 mRNA expression and androgen receptor (AR) localization. PBP C1 mRNA levels were measured by semi-quantitative in situ hybridization using a 35 S-labeled cDNA probe. In intact animals, strong hybridization signal could be observed in prostate sections after 12 hr of exposure to Kodak X-Omat films. In castrated rats, no PBP C1 mRNA could be detected even with longer exposure times, an effect that was prevented by administration of DHT. E 2 administered alone induced a detectable hybridization signal, and the concomitant administration of E 2 and DHT induced an increase in PBP C1 mRNA that significantly exceeded that obtained in animals that received only DHT. In prostate epithelial cells of intact animals, AR immunostaining was restricted to the nucleus. In castrated animals the alveoli were decreased in size and the epithelial cells were atrophied. AR staining was weak and was detected in both cytoplasm and nucleus. DHT administration completely obviated the effect of castration on epithelial cell histology and on AR immunostaining distribution and intensity. Interestingly, E 2 administration alone induced moderate hypertrophy of epithelial cells compared to the histological appearance of cells in untreated castrated rats. Moreover, in E 2 -treated animals the nuclear staining was much stronger than that detected in untreated castrated rats, whereas the cytoplasmic staining was not modified by the treatment. In animals that received both DHT and E 2 , the staining was similar to that seen in DHT-treated rats. These results suggest that E 2 can influence the activity of rat prostate epithelial cells by mechanisms that remain to be fully clarified.

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“…The initial prostatic volumes and weights were estimated from a nomogram (36). All dogs were sacrificed at the end of the experiment.…”

Section: Methodsmentioning

confidence: 99%

Effects of Cyproterone Acetate on Experimentally Induced Canine Prostatic Hyperplasia

Tunn¹,

Senge²,

Schenck³

et al. 1980

Urol Int

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The effect of 3α-androstanediol (3α-diol), 17β-estradiol (E₂) and cyproterone acetate (CA) on prostates in castrated beagle dogs were investigated by histologjcal and histochemical examinations. A significant increase of prostatic weight occurred after 6 months’ treatment with 3α-diol alone and in combination with E₂. Histologically and histochemically, two different types of prostatic enlargement were observed: first, administration of 3α-diol resulted in diffuse glandular hyperplasia with replacement of functional activity monitored by strongly positive reactions for acid phosphatase, aminopeptidase and zinc. Second, 3α-diol plus E₂ produced stratified squamous metaplasia with cystic lumina and loss of the typical morphological structure. These glands showed negative reactions for acid phosphatase, aminopeptidase and zinc. In both types of prostatic hyperplasia CA abolished epithelial or metaplastic proliferation and induced atrophy of glandular epithelium. In estrogenized dogs activation of the fibromuscular stroma was obvious. CA prevented prostatic hyperplasia by atrophying epithelial effects.

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Biochemical and Histological Studies on Prostates in Castrated Dogs After Treatment With Androstanediol, Oestradiol and Cyproterone Acetate

Cited by 60 publications

References 12 publications

Early effects of estrogen on the rat ventral prostate

Early effects of estrogen on the rat ventral prostate

Effects of Estradiol on Prostate Epithelial Cells in the Castrated Rat

Effects of Cyproterone Acetate on Experimentally Induced Canine Prostatic Hyperplasia

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