Biochemical and histological studies on various bone cell preparations

Nijweide, Peter J.; Plas, A. van der; Scherft, J. P.

doi:10.1007/bf02409485

Cited by 91 publications

(50 citation statements)

References 32 publications

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“…To obtain the two cell populations, calvaria of 16-d-old chick embryos were dissected and the períostea removed from both sides of the calvaria (17) . One cell population (PF) was isolated from the periostea, the other cell population (OB) from the remaining calvaria.…”

Section: Cell Isolationmentioning

confidence: 99%

“…Some additional (17) biochemical experiments were performed with 6-d-old monolayer cultures. Lactate was determined according to Lowry et al (12), DNA according to Karsten et al (9), and for CAMP a binding assay according to Lust et al (14) was applied to cAMP extracted from the cell cultures with 90% npropanol in water (8).…”

Section: Biochemical Determinationsmentioning

confidence: 99%

“…Several procedures have been used to obtain these different types of bone cell (13,17,20,21,23) . Generally, the isolated cells are cultured as a monolayer for a number of days and then used for biochemical studies.…”

mentioning

confidence: 99%

“…In our previous studies we have isolated various cell types (16,17) and characterized them on the basis of histological studies with light microscopy, transmission electron microscopy, and scanning electron microscopy on the tissues from which the cells were isolated, before and after isolation (17). In addition the isolated cells were studied biochemically during monolayer culture (17).…”

mentioning

confidence: 99%

“…In addition the isolated cells were studied biochemically during monolayer culture (17). The results of these studies led us to conclude tentatively that one of the populations of isolated cells was a population of osteoblasts or osteoblastlike cells.…”

mentioning

confidence: 99%

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Bone formation and calcification by isolated osteoblastlike cells.

Nijweide¹,

Gent²,

Haas³

et al. 1982

The Journal of Cell Biology

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Two cell populations were isolated from calvaria of chick embryos : PF cells were liberated by collagenase treatment from the periosteum, OB cells from the periosteum-free calvarium . Both populations were cultured in plastic culture dishes. After 6 d of culture, monolayers of each cell type either were scraped off the culture dishes, transplanted on the chorio-allantoic membrane of 7-d-old quail eggs, and cultured there for 6 d, or were used for biochemical experiments .OB transplants proved capable of producing calcified bone matrix, whereas PF transplants formed only fibrous tissue. Biochemically, OB cells showed high CAMP production in the presence of parathyroid hormone (PTH), whereas CAMP production was not stimulated in PF cultures . Lactate production was stimulated by PTH in both populations although somewhat differently . Citrate decarboxylation was high in OB cells and was inhibited by PTH but was low in PF cells, where it was stimulated by the same hormone . The differences in hormonal response between the two cell types made it possible to conclude that PF cultures are relatively free of OB cells. The PF contamination in OB cultures was more difficult to assess .The experiments described in this report show that the OB population contains osteoblasts or osteoblastlike cells which are, under favorable circumstances, capable of bone formation .

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Section: Cell Isolationmentioning

confidence: 99%

Section: Biochemical Determinationsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Bone formation and calcification by isolated osteoblastlike cells.

Nijweide¹,

Gent²,

Haas³

et al. 1982

The Journal of Cell Biology

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Regulation of cell-cell communication in rat bone cells: The effect of phorbol esters

1999

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The skin tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) is a potent inhibitor of gap junctional intercellular communication. In the present study, the inhibition of cell-cell communication by TPA has been investigated in primary bone cells from newborn rat calvaria, with an emphasis on the involvement of intracellular pH (pH(i)) and cytosolic calcium ([Ca(+2)](i)) in this process. The results show that TPA (5 x 10(-)(8) M) caused a complete inhibition of intercellular communication within 40-60 min. The intercellular communication was fully restored after overnight incubation in the presence of TPA. This effect was found to be associated with an elevation of pH(i). However, neither an increase of pH(i) alone nor exposure to TPA, under conditions preventing pH(i)-shift, were found to affect intercellular communication. It is suggested that the inhibition of intercellular communication, in the presence of TPA, depends on the pH(i)-shift itself rather than on the absolute value of pH(i). In addition, elevation of cytosolic calcium by ionomycin led to the termination of intercellular communication after 30 min. This inhibitory effect was abolished when the cells were incubated for overnight with TPA and then intracellular calcium was elevated by the addition of ionomycin. These results indicate that shift of pH(i) and the increase of intracellular calcium are involved in repression of intercellular communication by TPA.

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Osteoblastic Differentiation

Nijweide¹,

Plas²,

Olthof³

2007

Ciba Foundation Symposium 136 ‐ Cell and Molecular Biology of Vertebrate Hard Tissues

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Neonatal hyperbilirubinemia, which is prevalent among Asian peoples, has been considered as a physiological phenomenon, and its metabolic basis has not been clearly explained. Gilbert syndrome is a common inherited disease of unconjugated hyperbilirubinemia due to decreased bi-lirubin uridine diphosphate-glucuronosyltransferase (B-UGT), and its role in neonatal jaundice has recently been considered. We have previously reported that the Gly71Arg mutation of the B-UGT gene associated with Gilbert syndrome is prevalent in Japanese, Korean, and Chinese populations and was more frequently detected in neonates with severe hyperbilirubinemia than in control subjects. We have studied 159 Japanese full-term neonates, evaluating the relationship between the B-UGT genotype and the severity of jaundice, as assessed with a transcutaneous bilirubinometer. The gene frequency of the Gly71Arg mutation in these neonates was 0.19, and neonates carrying the Gly71Arg mutation had significantly increased bilirubin levels on days 2-4, manifested in a gene dose-dependent manner. The frequency of the Gly71Arg mutation was 0.47 in the neo-nates who required phototherapy (i.e., those with more severe hyperbilirubinemia), significantly higher than 0.16 in the neonates who did not require the therapy. The gene frequency of the TA repeat promoter polymorphism, the (TA) 7 mutation, was 0.07, and neonates carrying this mutation did not have an increase in bilirubin. These results suggested that the Gly71Arg mutation contributes to the high incidence of neonatal hyperbilirubinemia in Japanese.

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Biochemical and histological studies on various bone cell preparations

Cited by 91 publications

References 32 publications

Bone formation and calcification by isolated osteoblastlike cells.

Bone formation and calcification by isolated osteoblastlike cells.

Regulation of cell-cell communication in rat bone cells: The effect of phorbol esters

Osteoblastic Differentiation

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