Biochemical and histopathological responses of the Swiss albino mice treated with uranyl nitrate and its recovery

Vijayan, Periasamy; Rekha, P. D.; Dinesh, US; Arun, A. B.

doi:10.3109/0886022x.2016.1160248

Cited by 12 publications

(10 citation statements)

References 20 publications

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“…These assessments were qualitative, however, and did not provide measurements of uranium content. Other studies have reported tubular damage involving both the cortex and medulla of kidneys in mice injected with uranyl nitrate [ 8 ] or uranyl acetate [ 6 ] or in rats implanted with depleted uranium [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…Rats and mice exposed to uranium compounds by injection, inhalation or through drinking water, show signs of nephrotoxicity [ 4 ]. Studies of histological changes indicate the most damage in the corticomedullary region containing the pars recta segment of the nephron [ 5 – 8 ]. Previous analyses of uranium toxicity in the kidney are limited, however, by the lack of quantitative measurements of this element in different regions of the kidney.…”

Section: Introductionmentioning

confidence: 99%

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Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry

Jim¹,

LaViolette²,

Briehl³

et al. 2017

J Appl Bioanal

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The aim of the study is to better understand where uranium deposits in mice kidneys. The spatial distribution of uranium was examined in the kidneys of C57BL/6 mice using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Mice were exposed to varying levels of uranyl nitrate in their drinking water. Calibration standards were developed to allow for semi-quantitative measurement of uranium in the cortical and medullary regions of mice kidney by LA-ICP-MS. Scanning electron microscopy was used to image the ablation patterns on the kidney. Uranium levels were observed to increase in kidney tissue as uranyl nitrate treatment exposure levels increased. A trend towards a higher uranium concentration in the medullary versus cortical region of the kidneys was observed. These results show the usefulness of LA-ICP-MS in toxicity studies by providing a quantitative, spatial assessment of uranium deposition in a target organ.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry

Jim¹,

LaViolette²,

Briehl³

et al. 2017

J Appl Bioanal

View full text Add to dashboard Cite

show abstract

“…Collected urines are used to quantify uranium or fluoride, to measure specific and sensitive biomarkers of nephrotoxicity levels and to assess clinical bioassays. Finally, animals are euthanized 48h and 72h after exposure, chosen as the peak of nephrotoxicity is observed after intraperitoneal injection [3]. The protocol is shown in figure 1.…”

Section: Methods and Resultsmentioning

confidence: 99%

In vivo comparison between two nephrotoxic agents, sodium fluoride and uranyl nitrate: phenotypic aspects and molecular mechanisms involved

Bontemps¹,

Conquet²,

Elie³

et al. 2018

Toxicology Letters

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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“…Indeed, acute exposure to U induces nephrotoxicity in both humans and animals [6,14] and this impairment is mainly observed in proximal tubule renal epithelial cells [15,16]. After acute exposure of 1 to 5 mg/kg U injected intraperitoneally or intramuscularly, rodents demonstrated tubular necrosis and changes in blood chemistry reflecting severe renal alterations [16,17,18]. Similarly, intravenous acute exposures to F induce kidney damage in rats [19], even at low concentrations [20].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Comparison of the Phenotypic Aspects and Molecular Mechanisms of Two Nephrotoxic Agents, Sodium Fluoride and Uranyl Nitrate

Bontemps

Conquet

Elie

et al. 2019

IJERPH

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Because of their nephrotoxicity and presence in the environment, uranium (U) and fluoride (F) represent risks to the global population. There is a general lack of knowledge regarding the mechanisms of U and F nephrotoxicity and the underlying molecular pathways. The present study aims to compare the threshold of the appearance of renal impairment and to study apoptosis and inflammation as mechanisms of nephrotoxicity. C57BL/6J male mice were intraperitoneally treated with a single dose of U (0, 2, 4 and 5 mg/kg) or F (0, 2, 5, 7.5 and 10 mg/kg) and euthanized 72 h after. Renal phenotypic characteristics and biological mechanisms were evaluated by urine biochemistry, gene/protein expression, enzyme activity, and (immuno)histological analyses. U and F exposures induced nephrotoxicity in a dose-dependent manner, and the highest concentrations induced severe histopathological alterations as well as increased gene expression and urinary excretion of nephrotoxicity biomarkers. KIM-1 gene expression was induced starting at 2 mg/kg U and 7.5 mg/kg F, and this increase in expression was confirmed through in situ detection of this biomarker of nephrotoxicity. Both treatments induced inflammation as evidenced by cell adhesion molecule expression and in situ levels, whereas caspase 3/7-dependent apoptosis was increased only after U treatment. Overall, a single dose of F or U induced histopathologic evidence of nephrotoxicity renal impairment and inflammation in mice with thresholds under 7.5 mg/kg and 4 mg/kg, respectively.

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Biochemical and histopathological responses of the Swiss albino mice treated with uranyl nitrate and its recovery

Cited by 12 publications

References 20 publications

Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry

Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry

In vivo comparison between two nephrotoxic agents, sodium fluoride and uranyl nitrate: phenotypic aspects and molecular mechanisms involved

In Vivo Comparison of the Phenotypic Aspects and Molecular Mechanisms of Two Nephrotoxic Agents, Sodium Fluoride and Uranyl Nitrate

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