Biochemical and immunochemical properties of the cell surface of Renibacterium salmoninarum

Abstract

The biochemical composition of the cell envelope of Renibacterium salmoninarum was investigated in a total of 13 strains isolated from different salmonid fish species at various geographicil locations of the United States, Canada, and Europe. A marked similarity with the type strain R. salmoninarum ATCC 33209 was found both in the peptidoglycan and the cell wall polysaccharide. The primary structure of the peptidoglycan was found to be consistent with lysine in the third position of the peptide subunit, a glycyl-alanine interpeptide bridge between lysine and D-alanine of adjacent peptide subunits, and a D-alanine amide substituent at the a-carboxyl group of D-glutamic acid in position 2 of the peptide subunit. The cell wall polysaccharide contained galactose as the major sugar component which was accompanied by rhamnose, N-acetylglucosamine, and Nacetylfucosamine. The polysaccharide amounted to more than 60% of the dry weight of the cell walls. It was found to be covalently linked to the peptidoglycan and was released by hot formamide treatment. On gel filtration chromatography the extracted polysaccharide behaved like a homogeneous polymeric compound. The purified cell wall polysaccharide showed antigenic activity with antiserum obtained by immunization of rabbits with heat-inactivated trypsinized cells of R. salmoninarum. Immunoblotting experiments with nontrypsinized cell walls and antisera raised against R. salmoninarum cells revealed that antigenic proteins were attached to the cell walls.

Production of the 57 kDa major surface antigen by a non-agglutinating strain of the fish pathogen Renibacterium salmoninarum

“…E-mail: rstevens@micro.uoguelph.ca for electron microscopy. They suggested it was composed of the polysaccharide described by Fiedler & Draxl (1986), but did not confirm the result chemically. Firnbrae were observed on the surface of R. salmoninarum (Dubrueil et al 1990b) and, on the basis of immunogold staining with a polyclonal antiserum, it was suggested that these structures were composed of p57.…”

“…The capsule is lacking in the cryosections of MT 239, as would be expected if the p57 material is being released into the culture medium. If p57 does form such a capsule, this would explain the description by Fiedler & Draxl(1986) of a surface-associated carbohydrate surrounding R. salmoninarum, providing congruity between 2 ideas about the composition of the surface layer on renibacterial cells.…”

Genome Sequence of the Fish Pathogen Renibacterium salmoninarum Suggests Reductive Evolution away from an Environmental Arthrobacter Ancestor

“…4). Finally, strains of R. salmoninarum are highly homogeneous with respect to the overall genomic structure, biochemical properties, and surface antigens (24,27,53,68).…”

Description of Bogoriellaceae fam. nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov. and emendation of some families of the suborder Micrococcineae.

“…The widely used algorithms that transform pairwise 16S rDNA similarity values into dissimilarity values, including the compensation for multiple substitutions that may occur at the same site Schleifer & Kandler (1972), Kalakoutskii (1989), Collins et al (1989), Martin et al (1997), Prauser et al (1997), Schumann et al (1997) and Busse & Schumann (1999). Data for the Micrococcaceae from Schleifer & Kandler (1972), Collins (1982), Embley et al (1983), Jones & Collins (1986), Gerencser & Bowden (1986), Stackebrandt et al (1983), Schleifer (1986), Fiedler & Draxl (1986), Stackebrandt et al (1995) and Gvozdiak et al (1998) Intrasporangiaceae Intrasporangium…”