Biochemical and Immunohistochemical Detection of the Epidermal Growth-Factor Receptor (Egf-R) in Breast-Tumor Specimens

Mw, Beckmann; Tutschek, B.; Krüger, K. H.; Niederacher, Dieter; Risse, B.; Ruppert, Clemens; Hg, Schnürch; Bender, H. G.

doi:10.3892/ijo.3.2.389

Cited by 2 publications

(2 citation statements)

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“…Hematoxylin-eosin (H&E) staining was used for routine pathologic evaluation (diameter, margins, grading, histologic typing). Estrogen receptor (ER) and progesterone receptor (PgR) expression were determined by immunohistochemistry and scored as described previously (Beckmann et al, 1993). As a source of normal DNA either peripheral blood leukocytes, which were obtained from patients at the time of surgery, or sections of lymph nodes without tumor infiltration (evaluation of serial sections under light microscopy by pathologist) were used.…”

Section: Samplesmentioning

confidence: 99%

Patterns of allelic loss on chromosome 17 in sporadic breast carcinomas detected by fluorescent-labeled microsatellite analysis

Niederacher¹,

Picard²,

Roeyen³

et al. 1997

Genes Chromosom. Cancer

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Section: Samplesmentioning

confidence: 99%

Patterns of allelic loss on chromosome 17 in sporadic breast carcinomas detected by fluorescent-labeled microsatellite analysis

Niederacher¹,

Picard²,

Roeyen³

et al. 1997

Genes Chromosom. Cancer

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“…Darmstadt, Germany). A semiquantitative scoring was applied as described earlier [32]: score 0 = 0% positive cells, score 1 = <10% positive cells, score 2 = 10-50% positive cells, score 3 = > 50% positive cells; score 0 and 1 were considered as nega tive, score 2 and 3 as positive. For the HER-2/neu expression the peroxidase-antiperoxidasc method was applied using c-erbB-2 monoclonal Ab (Triton Diagnostics) as primary antibody, pig antirabbit IgG Ab as secondary' antibody (DAKO).…”

Section: Immunohistochemical Detection O F Egf-r and Iier-2/nettmentioning

confidence: 99%

Expression Analyses of Epidermal Growth Factor Receptor and HER-2/neu: No Advantage of Prediction of Recurrence or Survival in Breast Cancer Patients

et al. 1996

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The overexpression of two members of the erbB oncogene family – the epidermal growth factor receptor protein (EGF-R) and the HER-2/neu protein – in breast cancer has been investigated by numerous authors. Their exact role in breast cancer is still not defined. The simultaneous investigation of EGF-R and HER-2/neu in the same study population has only rarely been performed in breast cancer. Therefore, we analyzed the EGF-R and the HER-2/neu protein expression in 142 breast cancer specimens and 12 benign breast samples by immunohistochemistry. Results of the different expression analyses were compared with established clinicopathological parameters including estrogen and progesterone receptor status. In our study group EGF-R expression correlated with advanced tumor stage, axillary lymph node status and histological grade. HER-2/neu expression correlated with larger tumor size. Neither the evaluation of a single parameter of the erbB family nor the combination of both parameters showed a significant correlation with the disease-free and the overall survival of the individual patient with breast cancer. Only the expression of the progesterone receptor correlated with a better overall survival. Steroid hormone receptor expression and the expression of EGF-R and HER-2/neu seem to be two independent phenomena in our samples of breast cancer. The simultaneous evaluation of EGF-R and HER-2/neu expression did not lead to new information of prognostic relevance.

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Biochemical and Immunohistochemical Detection of the Epidermal Growth-Factor Receptor (Egf-R) in Breast-Tumor Specimens

Cited by 2 publications

References 0 publications

Patterns of allelic loss on chromosome 17 in sporadic breast carcinomas detected by fluorescent-labeled microsatellite analysis

Patterns of allelic loss on chromosome 17 in sporadic breast carcinomas detected by fluorescent-labeled microsatellite analysis

Expression Analyses of Epidermal Growth Factor Receptor and HER-2/neu: No Advantage of Prediction of Recurrence or Survival in Breast Cancer Patients

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