Biochemical and Immunohistochemical Studies on Tropomyosin and Glutamate Dehydrogenase in the Chicken Liver

Akutsu, Satonari; Miyazaki, Junichi

doi:10.2108/zsj.19.275

Cited by 10 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, in this study, Sc-GDH protein distributions altered after ammonia stress, which were mostly expressed in the cytoplasm endothelial cells of hepatocytes. Identical conclusions were found in the livers of chicken and mouse (Akutsu and Miyazaki, 2002;Vázquez-Martínez et al, 2017). Collectively, these findings indicate that Sc-GDH in the hepatopancreas of razor clam plays a key role in response to external ammonia entering the bodies.…”

Section: Discussionsupporting

confidence: 70%

Vital Role of Glutamate Dehydrogenase Gene in Ammonia Detoxification and the Association Between its SNPs and Ammonia Tolerance in Sinonovacula constricta

et al. 2021

View full text Add to dashboard Cite

Increasing evidence has revealed accumulated ammonia will cause adverse effects on the growth, reproduction, and survival of aquatic animals. As a marine benthic mollusk, the razor clam Sinonovacula constricta shows better growth and survival under high ammonia nitrogen environment. However, little is known about its adaptation mechanisms to high ammonia stress in an integrated mariculture system. In this study, we analyzed the association between the polymorphism of glutamate dehydrogenase gene (GDH), a key gene involved in ammonia nitrogen detoxification, and ammonia tolerance. The results showed that 26 and 22 single-nucleotide polymorphisms (SNPs) of GDH in S. constricta (denoted as Sc-GDH) were identified from two geographical populations, respectively. Among them, two SNPs (c.323T > C and c.620C > T) exhibited a significant and strong association with ammonia tolerance, suggesting that Sc-GDH gene could serve as a potential genetic marker for molecular marker–assisted selection to increase survival rate and production of S. constricta. To observe the histological morphology and explore the histocellular localization of Sc-GDH, by paraffin section and hematoxylin–eosin staining, the gills were divided into gill filament (contains columnar and flattened cells) and gill cilia, whereas hepatopancreas was made up of individual hepatocytes. The results of immunohistochemistry indicated that the columnar cells of gill filaments and the endothelial cells of hepatocytes were the major sites for Sc-GDH secretion. Under ammonia stress (180 mg/L), the expression levels of Sc-GDH were extremely significantly downregulated at 24, 48, 72, and 96 h (P < 0.01) after RNA interference. Thus, we can speculate that Sc-GDH gene may play an important role in the defense process against ammonia stress. Overall, these findings laid a foundation for further research on the adaptive mechanisms to ammonia–nitrogen tolerance for S. constricta.

show abstract

Section: Discussionsupporting

confidence: 70%

Vital Role of Glutamate Dehydrogenase Gene in Ammonia Detoxification and the Association Between its SNPs and Ammonia Tolerance in Sinonovacula constricta

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Several metabolic enzymes identified here have already been known to associate with actin fibers: glutamate dehydrogenase [33], glyceraldehyde-3-phosphate dehydrogenase (GAPDH) [34], pyruvate kinase [35], and enolase [36].…”

Section: Cytosolic Skeleton Fractionmentioning

confidence: 90%

“…The majority of the proteins that were not previously implicated to associate with the skeleton were metabolic enzymes (Table 1A): phosphoglycerate mutase (glycolysis), transketolase, phosphogluconate dehydrogenase (pentose phosphate pathway), n-acetylglucosamine kinase (glutamate and aminosugar metabolism), glucosidase (glycoprotein processing), and aldehyde dehydrogenase (oxidation of aldehydes). Several metabolic enzymes identified here have already been known to associate with actin fibers: glutamate dehydrogenase [33], glyceraldehyde-3-phosphate dehydrogenase (GAPDH) [34], pyruvate kinase [35], and enolase [36].…”

Section: Cytosolic Skeleton Fractionmentioning

confidence: 90%

Subproteome analysis of the neutrophil cytoskeleton

Crawford

Way

et al. 2009

Proteomics

View full text Add to dashboard Cite

Neutrophils play a key role in the early host-defense mechanisms due to their capacity to migrate into inflamed tissues and phagocytose microorganisms. The cytoskeleton has an essential role in these neutrophil functions, however, its composition is still poorly understood. We separately analyzed different cytoskeletal compartments: cytosolic skeleton, phagosome membrane skeleton, and plasma membrane skeleton. Using a proteomic approach, 138 nonredundant proteins were identified. Proteins not previously known to associate with the skeleton were: n-acetylglucosamine kinase, phosphoglycerate mutase 1, prohibitin, ficolin-1, phosphogluconate dehydrogenase, glucosidase, transketolase, major vault protein, valosin-containing protein, aldehyde dehydrogenase, and lung cancer-related protein-8 (LCRP8). The majority of these proteins can be classified as energy metabolism enzymes. Such a finding was interesting because neutrophil energy metabolism is unusual, mainly relying on glycolysis. The enrichment of phosphoglycerate mutase in cytosolic skeleton was additionally indicated by the use of Western blotting. This is the broadest subcellular investigation to date of the neutrophil cytoskeletal proteome and the first proteomic analysis in any cell type of the phagosome skeleton. The association of metabolic enzymes with cytoskeleton is suggestive of the importance of their localized enrichment and macromolecular organization in neutrophils.

show abstract

“…In muscle cells, Tm interacts with troponin T, caldesmon, and tropomodulin as part of the contractile apparatus (Perry, 2001). In non‐muscle cells, various Tm isoforms bind to calponin (Takahashi et al , 1986), pEL98 (Takenaga et al , 1994), and glutamic dehydrogenase (Akutsu and Miyazaki, 2002) although the physiological significance of these interactions is largely unknown. By linking Tm directly to RhoA signalling, we establish a new paradigm in which structurally unrelated actin‐binding proteins such as Tm and synaptopodin not only serve as passive structural components of actin filaments, but also can actively regulate stress fibre dynamics (Figure 7E).…”

Section: Discussionmentioning

confidence: 99%

Rescue of tropomyosin deficiency inDrosophilaand human cancer cells by synaptopodin reveals a role of tropomyosin α in RhoA stabilization

et al. 2011

View full text Add to dashboard Cite

Tropomyosins are widespread actin‐binding proteins that influence numerous cellular functions including actin dynamics, cell migration, tumour suppression, and Drosophila oocyte development. Synaptopodin is another actin‐binding protein with a more restricted expression pattern in highly dynamic cell compartments such as kidney podocyte foot processes, where it promotes RhoA signalling by blocking the Smurf1‐mediated ubiquitination of RhoA. Here, we show that synaptopodin has a shorter half‐life but shares functional properties with the highly stable tropomyosin. Transgenic expression of synaptopodin restores oskar mRNA localization in Drosophila oocytes mutant for TmII, thereby rescuing germline differentiation and fertility. Synaptopodin restores stress fibres in tropomyosin‐deficient human MDA‐MB 231 breast cancer cells and TPMα‐depleted fibroblasts. Gene silencing of TPMα but not TPMβ causes loss of stress fibres by promoting Smurf1‐mediated ubiquitination and proteasomal degradation of RhoA. Functionally, overexpression of synaptopodin or RhoA(K6,7R) significantly reduces MDA‐MB 231 cell migration. Our findings elucidate RhoA stabilization by structurally unrelated actin‐binding proteins as a conserved mechanism for regulation of stress fibre dynamics and cell motility in a cell type‐specific fashion.

show abstract

Biochemical and Immunohistochemical Studies on Tropomyosin and Glutamate Dehydrogenase in the Chicken Liver

Cited by 10 publications

References 42 publications

Vital Role of Glutamate Dehydrogenase Gene in Ammonia Detoxification and the Association Between its SNPs and Ammonia Tolerance in Sinonovacula constricta

Vital Role of Glutamate Dehydrogenase Gene in Ammonia Detoxification and the Association Between its SNPs and Ammonia Tolerance in Sinonovacula constricta

Subproteome analysis of the neutrophil cytoskeleton

Rescue of tropomyosin deficiency inDrosophilaand human cancer cells by synaptopodin reveals a role of tropomyosin α in RhoA stabilization

Contact Info

Product

Resources

About