Biochemical and immunological analysis of mould skin prick test solution: current status of standardization

Kespohl, S.; Maryska, S.; Zahradnik, Eva; Sander, I.; Brüning, Thomas; Raulf‐Heimsoth, Monika

doi:10.1111/cea.12186

Cited by 50 publications

(49 citation statements)

References 35 publications

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“…depth of puncture), status of skin reactivity, application devices , patients’ medication and others . In our previous study , a biochemical analysis of mould SPT solutions revealed both quantitative and qualitative differences depending not only on the mould species, but more specifically on the manufacturer. The questions arising from that analysis were the following: do these differences in extract composition also reveal differences in SPT results, and how good are the correlations/concordances between SPT from different manufacturers and the sIgE test?…”

Section: Introductionmentioning

confidence: 87%

How to diagnose mould allergy? Comparison of skin prick tests with specific IgE results

Kespohl

Maryska

Bünger

et al. 2016

Clin Experimental Allergy

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Section: Introductionmentioning

confidence: 87%

How to diagnose mould allergy? Comparison of skin prick tests with specific IgE results

Kespohl

Maryska

Bünger

et al. 2016

Clin Experimental Allergy

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“…Previous studies have demonstrated significant variation in allergen content of commercial SPT extracts for birch and grass pollen, mould and occupational allergens . A large heterogenicity in in vivo and in vitro reactivity and allergen content among five commercial crustacean extracts was recently demonstrated .…”

Section: Introductionmentioning

confidence: 98%

Variability of allergens in commercial fish extracts for skin prick testing

Ruethers

Taki

Nugraha

et al. 2019

Allergy

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Background: Commercial allergen extracts for allergy skin prick testing (SPT) are widely used for diagnosing fish allergy. However, there is currently no regulatory requirement for standardization of protein and allergen content, potentially impacting the diagnostic reliability of SPTs. We therefore sought to analyse commercial fish extracts for the presence and concentration of fish proteins and in vitro IgE reactivity using serum from fish-allergic patients.Methods: Twenty-six commercial fish extracts from five different manufacturers were examined. The protein concentrations were determined, protein compositions analysed by mass spectrometry, followed by SDS-PAGE and subsequent immunoblotting with antibodies detecting 4 fish allergens (parvalbumin, tropomyosin, aldolase and collagen). IgE-reactive proteins were identified using serum from 16 children with confirmed IgE-mediated fish allergy, with focus on cod, tuna and salmon extracts. Results:The total protein, allergen concentration and IgE reactivity of the commercial extracts varied over 10-fold between different manufacturers and fish species. The major fish allergen parvalbumin was not detected by immunoblotting in 6/26 extracts. In 7/12 extracts, five known fish allergens were detected by mass spectrometry. For cod and tuna, almost 70% of patients demonstrated the strongest IgE reactivity to collagen, tropomyosin, aldolase A or β-enolase but not parvalbumin.

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“…Commercially available extracts may lack important allergens or may vary considerably in their allergen composition, leading to false-negative test results for certain patients. Varying allergen compositions and contents have been shown for skin prick test (SPT) solutions for the house dust mite Dermatophagoides pteronyssinus [29], the mould Alternaria alternata [30], and hazelnut [31] as well as for sublingual allergen immunotherapy products for house dust mite, storage mite, and birch pollen [32,33]. At least for routine diagnostics, a process called spiking (i.e., the addition of recombinant allergens) has been used to improve the quality of allergenic extracts such as latex [34], hazelnut [35], and wasp venom [36].…”

Section: Recombinant Allergens In Diagnostic Testsmentioning

confidence: 99%

Recombinant Allergens in Structural Biology, Diagnosis, and Immunotherapy

Tscheppe

Breiteneder²

2017

Int Arch Allergy Immunol

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The years 1988-1995 witnessed the beginning of allergen cloning and the generation of recombinant allergens, which opened up new avenues for the diagnosis and research of human allergic diseases. Most crystal and solution structures of allergens have been obtained using recombinant allergens. Structural information on allergens allows insights into their evolutionary biology, illustrates clinically observed cross-reactivities, and makes the design of hypoallergenic derivatives for allergy vaccines possible. Recombinant allergens are widely used in molecule-based allergy diagnosis such as protein microarrays or suspension arrays. Recombinant technologies have been used to produce well-characterized, noncontaminated vaccine components with known biological activities including a variety of allergen derivatives with reduced IgE reactivity. Such recombinant hypoallergens as well as wild-type recombinant allergens have been used successfully in several immunotherapy trials for more than a decade to treat birch and grass pollen allergy. As a more recent application, the development of antibody repertoires directed against conformational epitopes during immunotherapy has been monitored by recombinant allergen chimeras. Although much progress has been made, the number and quality of recombinant allergens will undoubtedly increase and keep improving our knowledge in basic scientific investigations, diagnosis, and therapy of human allergic diseases.

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Biochemical and immunological analysis of mould skin prick test solution: current status of standardization

Abstract: Commercially available mould SPT extracts showed high variability raising the question of comparability and reliability of SPT results. Possible consequences for diagnostic test outcome will be investigated in the next step.

Cited by 50 publications

References 35 publications

How to diagnose mould allergy? Comparison of skin prick tests with specific IgE results

How to diagnose mould allergy? Comparison of skin prick tests with specific IgE results

Variability of allergens in commercial fish extracts for skin prick testing

Recombinant Allergens in Structural Biology, Diagnosis, and Immunotherapy

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