Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products

He, Lei; Ren, Mengyu; Chen, Xueqing; Wang, Xiaoyun; Li, Shan; Lin, Jintian; Liang, Chi; Liang, Pei; Hu, Yue; Lei, Huali; Bian, Meng; Huang, Yan; Wu, Zhongdao; Li, Xuerong; Yu, Xinbing

doi:10.1007/s00436-014-3935-4

Cited by 23 publications

(21 citation statements)

References 66 publications

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“…TmAnxB3 was mainly distributed in the tegument of each segment in T. multiceps , and was also widely distributed in the parenchymatous zone of the scolex and neck. These findings are consistent with the known distribution of annexins in E. granulosus , Clonorchis sinensis and S. mansoni [ 21 , 22 , 34 ]. The tegument of parasitic cestodes not only plays a key role in escaping the attack from host immune system, it is also an important organ for nutrient acquisition and signal transduction during long-term parasitic life [ 12 ].…”

Section: Discussionsupporting

confidence: 91%

“…Importantly, these proteins contribute to physiological functions in taeniidae in a tissue-specific manner [ 36 , 37 ]. For instance, AnxB30 of C. sinensis is transcribed at high levels in metacercaria and at lower levels in adults and eggs compared to other stages [ 34 ]. However, ANXB33 of E. granulosus is mainly transcribed in the germinal layer and protoscoleces, but not expressed in the cystic fluid or cyst wall [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

Guo

Xie

Liu

et al. 2018

Genes

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Coenurus cerebralis, the metacestode of Taenia multiceps, causes coenurosis, a disease severely affecting goat, sheep, cattle and yak farming and resulting in huge economic losses annually. Annexins bind calcium ions and play an important role in flatworm parasite development. To explore potential functions of annexins in T. multiceps, three homologous genes, namely, TmAnxB2, TmAnxB3 and TmAnxB12, were screened from the transcriptome dataset, amplified from C. cerebralis cDNA and subjected to bioinformatics analysis. Then, polyclonal antibodies recognizing the recombinant TmAnxB2 (rTmAnxB2) and rTmAnxB3 were prepared for localization of TmAnxB2 and TmAnxB3 in different tissues and developmental stages by immunofluorescence. The transcription of all three genes was also measured by relative fluorescent quantitative PCR. The sizes of rTmAnxB2, rTmAnxB3 and rTmAnxB12 were 58.00, 53.06 and 53.51 kDa, respectively, and rTmAnxB12 was unstable. Both rTmAnxB2 and rTmAnxB3 were recognized by goat-positive T. multiceps sera in Western blots. Immunofluorescence revealed that TmAnxB2 and TmAnxB3 were localized in the protoscolex and cyst wall and TmAnxB3 was also detected in adult cortex. TmAnxB2 and TmAnxB12 mRNA levels were determined to be highest in oncospheres and protoscolex, whereas transcription of TmAnxB3 was highest in scolex and immature segments. Taken together, these findings indicate that TmAnxB2 and TmAnxB12 may play critical roles in T. multiceps larvae, while TmAnxB3 may have important functions in adults. These results will lay the foundation for functional research of annexins in T. multiceps.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

Guo

Xie

Liu

et al. 2018

Genes

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“…16,17,33,34 Our bioinformatic analysis showed that Eg-ANX has lost Ca 2+ -binding sites in repeats II and III, and this loss was Immunofluorescence localization of Eg-ANX in different stages of E. granulosus. Eg-ANX in the protoscolex, germinal layer, and adult was immunofluorescently labeled using specific anti-rEg-ANX IgG (positive), or control pre-immune serum (negative), followed by fluorescein isothiocyanate (FITC)-conjugated anti-rabbit IgG.…”

Section: Discussionmentioning

confidence: 84%

“…16 Annexin B30 of Clonorchis sinensis was shown to be involved in host-parasite interaction and affected the immune response of the host during C. sinensis infection. 17 Interestingly, some annexins without any secretory signals have been found in the extracellular space (e.g., annexin A1, A2, and A5 of human and annexin B1 of T. solium). 15,[18][19][20] The mechanism by which this occurs remains unclear and is worthy of investigation.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Secretory Annexin in Echinococcus granulosus

Song¹,

Hu²,

Zhong³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Cystic echinococcosis, caused by Echinococcus granulosus, is a widespread parasitic zoonosis causing economic loss and public health problems. Annexins are important proteins usually present in the plasma membrane, but previous studies have shown that an annexin B33 protein of E. granulosus (Eg-ANX) could be detected in the excretory/ secretory products and cyst fluid. In this study, we cloned and characterized Eg-ANX. In silico analysis showed that the amino acid sequence of Eg-ANX was conserved and lacked any signal peptides. The phospholipid-binding activity of recombinant Eg-ANX (rEg-ANX) was tested; liposomes could bind to rEg-ANX only in the presence of Ca 2+

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“…Other examples of helminth infections where parasite proteins have been involved in Plg/Plm binding as an evasion mechanism of the host’s innate defensive response are Clonorchis sinensis , in which GAPDH [ 136 ] and annexin B30 have been reported as Plg-binding proteins [ 137 ]. Enolases have also been reported as Plg-binding proteins in Onchocerca volvulus [ 134 ], Fasciola hepatica [ 138 ], Taenia multiceps [ 140 ], and T. pisiformis [ 22 ].…”

Section: Role Of Plg Receptors In Sterile and Non-sterile Conditionsmentioning

confidence: 99%

Plasminogen-binding proteins as an evasion mechanism of the host’s innate immunity in infectious diseases

et al. 2018

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Pathogens have developed particular strategies to infect and invade their hosts. Amongst these strategies’ figures the modulation of several components of the innate immune system participating in early host defenses, such as the coagulation and complement cascades, as well as the fibrinolytic system. The components of the coagulation cascade and the fibrinolytic system have been proposed to be interfered during host invasion and tissue migration of bacteria, fungi, protozoa, and more recently, helminths. One of the components that has been proposed to facilitate pathogen migration is plasminogen (Plg), a protein found in the host’s plasma, which is activated into plasmin (Plm), a serine protease that degrades fibrin networks and promotes degradation of extracellular matrix (ECM), aiding maintenance of homeostasis. However, pathogens possess Plg-binding proteins that can activate it, therefore taking advantage of the fibrin degradation to facilitate establishment in their hosts. Emergence of Plg-binding proteins appears to have occurred in diverse infectious agents along evolutionary history of host–pathogen relationships. The goal of the present review is to list, summarize, and analyze different examples of Plg-binding proteins used by infectious agents to invade and establish in their hosts. Emphasis was placed on mechanisms used by helminth parasites, particularly taeniid cestodes, where enolase has been identified as a major Plg-binding and activating protein. A new picture is starting to arise about how this glycolytic enzyme could acquire an entirely new role as modulator of the innate immune system in the context of the host–parasite relationship.

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Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products

Cited by 23 publications

References 66 publications

Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

Characterization of a Secretory Annexin in Echinococcus granulosus

Plasminogen-binding proteins as an evasion mechanism of the host’s innate immunity in infectious diseases

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