Biochemical and metabolic profiles in in vivo- and in vitro-derived concepti in cattle

Ticiani, Elvis; Rodrigues, Victor Hugo Vieira; Willhelm, Bruna Rodrigues; Ribeiro, E.S.; Gerger, Renato Pereira da Costa; Miglino, María Angélica; Ambrósio, Carlos Eduardo; Ferrell, C. L.; Sainz, Roberto Daniel; Rodrigues, José Luiz; Bertolini, Marcelo

doi:10.1016/j.livsci.2020.103947

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…Perhaps, as Flemish calves tend to be heavier than other breeds at birth, fructose levels become similar between calves from both groups, as all calves, irrespective of the group, had rather large birth weights (>40 kg). Also, fructose concentrations in the plasma of IVP and IVD neonates (6 to 10 mM) in this study seemed to be somewhat higher than those reported for IVP‐derived calves (4 to 6 mM) from our previous studies, but similar to values observed for cloned calves (5 to 10 mM), which usually demonstrate a wide range of abnormalities, including unusually large birth weights (Batchelder et al., 2007; Bertolini et al., 2004; Gerger et al., 2017; Ticiani et al., 2020). Interestingly, extreme clinical consequences of high fructose levels could not be detected in this study due to rather mild transient respiratory distress after birth, subtle changes in acid–base balance, and perhaps due to faster fructose clearance, as components of the fructolytic pathway, along with LDH‐B, were upregulated, as mentioned above.…”

Section: Discussionsupporting

confidence: 86%

“…In this study, IVP calves had altered lipid metabolism, presenting higher plasma HDL, triglycerides, total cholesterol, and HDL levels at several time points during the first 24 h of life than control animals. An increase in components of the lipid metabolism has been previously reported in the plasma of bovine cloned foetuses (Ticiani et al, 2020), indicating that IVP of embryos may alter energy metabolism of the conceptus and the newborn calves. Moreover, lipid mobilization may be an alternative mechanism that gradually compensate for negative energy balance early after birth (Schäff et al, 2014), which is exacerbated in large calves.…”

Section: Discussionmentioning

confidence: 76%

“…Although blood energy substrate levels did not differ between experimental groups in this study, the patterns of gene expression for triokinase, the transcript for an enzyme of the fructolytic pathway, and for lactate dehydrogenase‐B (LDH‐B), which encodes for an enzyme that catalyses the reversible conversion of pyruvate into lactate, were increased in the liver of IVP calves, suggesting changes in energy metabolism at the molecular level. We have already demonstrated that larger IVP concepti and calves usually also have increased plasma fructose levels, which may be relevant to survival (Batchelder et al., 2007; Bertolini et al., 2004; Gerger et al., 2017; Ticiani et al., 2020). Perhaps, as Flemish calves tend to be heavier than other breeds at birth, fructose levels become similar between calves from both groups, as all calves, irrespective of the group, had rather large birth weights (>40 kg).…”

Section: Discussionmentioning

confidence: 96%

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Analysis of physical traits, clinical parameters, and energy metabolism of in vivo‐ and in vitro‐derived Flemish newborn calves during the first day of life

Schütz,

Zago,

de Aguiar

et al. 2024

Reprod Domestic Animals

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Studies investigating physiological deviations from normality in newborn calves derived from in vitro fertilization procedures remain important for the understanding of factors that reduce calf survival after birth. The aim of this study was to investigate parameters affecting health and welfare of newborn Flemish calves derived from in vitro embryo production (IVP) in the first hours of life in comparison to in vivo‐derived calves. Physical traits of newborn calves and fetal membranes (FM) were recorded soon after birth. Newborn venous blood samples were collected at several time points within the first 24 h of life for analyses of energy substrates, electrolytes, blood gases, acid–base balance, blood chemistry, and haematology. A liver biopsy was taken within the first hour after birth for analysis of gene expression of key enzymes of the fructolytic and glycolytic pathways. Newborn IVP calves were heavier and larger at birth, which was associated with heavier FM. At several time points during the first 24 h of life, IVP‐derived calves had altered rectal temperature, blood gases, electrolyte concentrations, blood parameters for liver, kidney and muscle function, and acid–base balance, plasma lipid metabolism, and hemogram parameters. The relative mRNA abundances for triokinase and lactate dehydrogenase‐B were greater in IVP calves. In summary, IVP‐derived newborn calves were at higher risk of clinical problems after birth, which was markedly greater in heavier and larger calves. Such animals take longer to adapt to extrauterine life and should receive a special attention during the immediate neonatal period.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 96%

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Analysis of physical traits, clinical parameters, and energy metabolism of in vivo‐ and in vitro‐derived Flemish newborn calves during the first day of life

Schütz,

Zago,

de Aguiar

et al. 2024

Reprod Domestic Animals

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“…The levels of the purine metabolism-related molecules uric acid, xanthine and hypoxanthine in the SCNT-DW4 group were dramatically higher than those in the AI-SO4 and SCNT-SO4 groups; this result was consistent with our previous result showing that SCNT-DW4 neonates are plagued by abnormal purine metabolism mainly in the form of elevated uric acid levels (Ao et al, 2020). Research in cloned cattle also found foetal plasma uric acid concentrations were higher in clones when compared with the in vivo group (Ticiani et al, 2020).…”

Section: Ta B L Esupporting

confidence: 90%

Associations of cord metabolome and biochemical parameters with the neonatal deaths of cloned pigs

Zhao

et al. 2021

Reprod Domestic Animals

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Genetically engineered pigs generated through somatic cell nuclear transfer (SCNT) offer valuable applications in agriculture and biomedicine (Lee, Farrell, & Uh, 2020; Yang & Wu, 2018). However, the high stillbirth and malformation incidences and neonatal mortality rates of cloned pigs are the well-recognized problems (Schmidt, Winther, Secher, & Callesen, 2015). A growing body of evidence suggests that cloned pigs undergo abnormal development during their intrauterine life that contributes to the high loss of neonates and

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“…Glucose is the primary energy substrate for the placenta and fetus and is essential for normal fetal metabolism and growth [ 16 ]. Fructose is the main carbohydrate in plasma and fetal fluids of mammals and may induce cell proliferation via activation of the mammalian target of rapamycin pathway [ 3 , 17 ]. Thus, one of the goals of this study is to investigate how glucose and fructose concentrations in fetal fluids are affected by nutritional management strategies.…”

Section: Introductionmentioning

confidence: 99%

Vitamin and Mineral Supplementation and Rate of Gain in Beef Heifers I: Effects on Dam Hormonal and Metabolic Status, Fetal Tissue and Organ Mass, and Concentration of Glucose and Fructose in Fetal Fluids at d 83 of Gestation

Menezes

McCarthy

Kassetas

et al. 2022

Animals

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Thirty-five crossbred Angus heifers (initial BW = 359.5 ± 7.1 kg) were randomly assigned to a 2 × 2 factorial design to evaluate effects of vitamin and mineral supplementation [VMSUP; supplemented (VTM) vs. unsupplemented (NoVTM)] and different rates of gain [GAIN; low gain (LG), 0.28 kg/d, vs. moderate gain (MG), 0.79 kg/d] during the first 83 d of gestation on dam hormone and metabolic status, fetal tissue and organ mass, and concentration of glucose and fructose in fetal fluids. The VMSUP was initiated 71 to 148 d before artificial insemination (AI), allowing time for mineral status of heifers to be altered in advance of breeding. At AI heifers were assigned their GAIN treatment. Heifers received treatments until the time of ovariohysterectomy (d 83 ± 0.27 after AI). Throughout the experiment, serum samples were collected and analyzed for non-esterified fatty acids (NEFA), progesterone (P4), insulin, and insulin-like growth factor 1 (IGF-1). At ovariohysterectomy, gravid reproductive tracts were collected, measurements were taken, samples of allantoic (ALF) and amniotic (AMF) fluids were collected, and fetuses were dissected. By design, MG had greater ADG compared to LG (0.85 vs. 0.34 ± 0.04 kg/d, respectively; p < 0.01). Concentrations of NEFA were greater for LG than MG (p = 0.04) and were affected by a VMSUP × day interaction (p < 0.01), with greater concentrations for NoVTM on d 83. Insulin was greater for NoVTM than VTM (p = 0.01). A GAIN × day interaction (p < 0.01) was observed for IGF-1, with greater concentrations for MG on d 83. At d 83, P4 concentrations were greater for MG than LG (GAIN × day, p < 0.01), and MG had greater (p < 0.01) corpus luteum weights versus LG. Even though fetal BW was not affected (p ≥ 0.27), MG fetuses had heavier (p = 0.01) femurs than LG, and VTM fetuses had heavier (p = 0.05) livers than those from NoVTM. Additionally, fetal liver as a percentage of BW was greater in fetuses from VTM (P = 0.05; 3.96 ± 0.06% BW) than NoVTM (3.79 ± 0.06% BW), and from LG (p = 0.04; 3.96 ± 0.06% BW) than MG (3.78 ± 0.06% BW). A VMSUP × GAIN interaction was observed for fetal small intestinal weight (p = 0.03), with VTM-MG being heavier than VTM-LG. Therefore, replacement heifer nutrition during early gestation can alter the development of organs that are relevant for future offspring performance. These data imply that compensatory mechanisms are in place in the developing conceptus that can alter the growth rate of key metabolic organs possibly in an attempt to increase or decrease energy utilization.

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Biochemical and metabolic profiles in in vivo- and in vitro-derived concepti in cattle

Cited by 6 publications

References 28 publications

Analysis of physical traits, clinical parameters, and energy metabolism of in vivo‐ and in vitro‐derived Flemish newborn calves during the first day of life

Analysis of physical traits, clinical parameters, and energy metabolism of in vivo‐ and in vitro‐derived Flemish newborn calves during the first day of life

Associations of cord metabolome and biochemical parameters with the neonatal deaths of cloned pigs

Vitamin and Mineral Supplementation and Rate of Gain in Beef Heifers I: Effects on Dam Hormonal and Metabolic Status, Fetal Tissue and Organ Mass, and Concentration of Glucose and Fructose in Fetal Fluids at d 83 of Gestation

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