Biochemical and Molecular Aspects Involved in Waterlogging Tolerance in Prunus Rootstocks

Amador, María L.; Sancho, S.; Rubio-Cabetas, M. J.

doi:10.17660/actahortic.2009.814.121

Cited by 10 publications

(3 citation statements)

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“…We cannot conclude that antioxidant enzymes are involved directly in the higher tolerance of ‘P.2175’ or in the scavenging of ROS compound. On the contrary, tolerance could be more related to fermentative pathways that better modulate the toxic accumulation of acetaldehyde and ethanol (Amador et al 2009). Ultimately, the whole photosynthetic machinery seems to play a more important role than the antioxidants system in the tolerance response (Vu and Yelenosky 1991, Arbona et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Physiological and biochemical parameters controlling waterlogging stress tolerance in Prunus before and after drainage

Amador

Sancho

Bielsa

et al. 2012

Physiologia Plantarum

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Waterlogging is associated with poor soil drainage. As a consequence oxygen levels decrease in the root environment inducing root asphyxia and affecting plant growth. Some plants can survive under these conditions triggering complex anatomical and biochemical adaptations, mostly in the roots. Long- and short-term responses to waterlogging stress were compared in two trials using a set of two myrobalans (Prunus cerasifera Erhr), 'P.2175' and 'P.2980', as tolerant rootstocks and two almond × peach [Prunus amygdalus Batsch ×Prunus persica (L.) Batsch] interspecific hybrids, 'Garnem' and 'Felinem', as sensitive ones in two consecutive years. Stomatal conductance and chlorophyll content were measured in the long-term trials to assess survival performance, while the enzyme activities of superoxide dismutase (SOD, EC 1.15.1.1), guaiacol peroxidase (POD, EC 1.11.1.7), and catalase (CAT, EC 1.11.1.6) were measured in the short-term trials to study early antioxidant response. The incidence of the stress in the root environment was different as a result of the different plant development at the moment of the treatment, as a consequence of different environmental conditions both before and during the treatment between the 2 years. The activity of the different enzymes was higher in the sensitive genotype 'Felinem' than in the tolerant 'P.2175'. This result shows an activation of the antioxidant system and has been observed to depend of the different nature of the roots between the 2 years. As the antioxidant enzymes seem to work more efficiently when roots are more aerated, we cannot conclude that they are responsible for the higher tolerance observed in the myrobalan plums.

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Section: Discussionmentioning

confidence: 99%

Physiological and biochemical parameters controlling waterlogging stress tolerance in Prunus before and after drainage

Amador

Sancho

Bielsa

et al. 2012

Physiologia Plantarum

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“…Clonally propagated and virus-free rootstocks, Mariana 2624 ( P. cerasifera × P. munsoniana) and Mazzard F12/1 ( P. avium ), which are respectively tolerant and sensitive to hypoxia conditions 23 , 47 – 49 , were acquired from a commercial nursery (Agromillora Sur, S.A., Curicó, Chile). The plants were transplanted to 2-L plastic pots with a mixture of vermiculite, perlite and sand (1:1:1v/v) as a substrate.…”

Section: Methodsmentioning

confidence: 99%

Prunus Hexokinase 3 genes alter primary C-metabolism and promote drought and salt stress tolerance in Arabidopsis transgenic plants

Pérez-Díaz

Batista‐Silva

Almada

et al. 2021

Sci Rep

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Hexokinases (HXKs) and fructokinases (FRKs) are the only two families of enzymes in plants that have been identified as able to phosphorylate Glucose (Glc) and Fructose (Fru). Glc can only be phosphorylated in plants by HXKs, while Fru can be phosphorylated by either HXKs or FRKs. The various subcellular localizations of HXKs in plants indicate that they are involved in diverse functions, including anther dehiscence and pollen germination, stomatal closure in response to sugar levels, stomatal aperture and reducing transpiration. Its association with modulating programmed cell death, and responses to oxidative stress and pathogen infection (abiotic and biotic stresses) also have been reported. To extend our understanding about the function of HXK-like genes in the response of Prunus rootstocks to abiotic stress, we performed a detailed bioinformatic and functional analysis of hexokinase 3-like genes (HXK3s) from two Prunus rootstock genotypes, ‘M.2624’ (Prunus cerasifera Ehrh × P. munsoniana W.Wight & Hedrick) and ‘M.F12/1’ (P. avium L.), which are tolerant and sensitive to hypoxia stress, respectively. A previous large-scale transcriptome sequencing of roots of these rootstocks, showed that this HXK3-like gene that was highly induced in the tolerant genotype under hypoxia conditions. In silico analysis of gene promoters from M.2624 and M.F12/1 genotypes revealed regulatory elements that could explain differential transcriptional profiles of HXK3 genes. Subcellular localization was determinates by both bioinformatic prediction and expression of their protein fused to the green fluorescent protein (GFP) in protoplasts and transgenic plants of Arabidopsis. Both approaches showed that they are expressed in plastids. Metabolomics analysis of Arabidopsis plants ectopically expressing Prunus HXK3 genes revealed that content of several metabolites including phosphorylated sugars (G6P), starch and some metabolites associated with the TCA cycle were affected. These transgenic Arabidopsis plants showed improved tolerance to salt and drought stress under growth chamber conditions. Our results suggest that Prunus HXK3 is a potential candidate for enhancing tolerance to salt and drought stresses in stone fruit trees and other plants.

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“…The difference in fl ooding tolerance found among Prunus species other than peach is based on complex anatomical processes such as aerenchyma formation and biochemical adaptation involving the fermentative pathways to obtain energy. Several candidate genes have been identifi ed to be involved in the tolerance in two Prunus genotypes (Amador et al 2009 ;Amador 2010 ) . Various plum and interspecifi c hybrids have been reported to be tolerant of waterlogged soils (Table 14. Tree architecture .…”

Section: Major Traits In Peach Scion Breedingmentioning

confidence: 99%

Peach

et al. 2011

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The peach is the third most produced temperate tree fruit species behind apple and pear. This diploid species, Prunus persica , is naturally self-pollinating unlike most of the other cultivated Prunus species. Its center of diversity is in China, where it was domesticated. Starting about 3,000 years ago, the peach was moved from China to all temperate and subtropical climates within the Asian continent and then, more than 2,000 years ago, spread to Persia (present day Iran) via the Silk Road and from there throughout Europe. From Europe it was taken by the Spanish and Portuguese explorers to the Americas. It has an extensive history of breeding

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Biochemical and Molecular Aspects Involved in Waterlogging Tolerance in Prunus Rootstocks

Cited by 10 publications

References 0 publications

Physiological and biochemical parameters controlling waterlogging stress tolerance in Prunus before and after drainage

Physiological and biochemical parameters controlling waterlogging stress tolerance in Prunus before and after drainage

Prunus Hexokinase 3 genes alter primary C-metabolism and promote drought and salt stress tolerance in Arabidopsis transgenic plants

Peach

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