Biochemical and molecular effects of naringenin on the cardiovascular oxidative and pro-inflammatory effects of oral exposure to diesel exhaust particles in rats

Oluyede, Dare M.; Lawal, Akeem O.; Adebimpe, Monsurat O.; Olumegbon, Lateefat T.; Elekofehinti, Olusola Olalekan

doi:10.1007/s11869-021-00991-2

Cited by 8 publications

(8 citation statements)

References 84 publications

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“…We observed in this study that LPS up-regulates the expression of IκKβ and NF-κβ genes, but down-regulates Iκβ gene expression, thereby triggering the activation and translocation of NF-κβ to the nucleus. This suggests that the pro-inflammatory effect of toxicants generally, and LPS in particular, is likely mediated via the canonical NF-κβ-signaling cascade, in accordance with reports from several earlier studies [ 18 , 73 ]. It is noteworthy in this study that RPO pre-feeding for four weeks inhibited the activation of NF-κβ signaling as indicated by a decrease in the gene expression of IκKβ and NF-κβ, parallel with an increase in Iκβ expression.…”

Section: Discussionsupporting

confidence: 91%

“…The activation of NF-κβ, a master regulator of inflammatory response in the cell is triggered by increased oxidative stress. Evidence has shown that NF-κβ can be activated via two separate pathways: the canonical pathway with IL-1, TNFα and Toll-like receptor (TLR) cytokines acting as triggers, and the non-canonical pathway where other TNF-family cytokines, β-cell activating factor, and receptor activator of NF-κβ ligand served as triggers [ 18 , 70 , 71 ]. In the canonical pathway, oxidative stress induces the activation of IκKβ which causes phosphorylation of Iκβ and subsequent release and nuclear translocation of NF-κβ, leading to the transcription of pro-inflammatory mediators [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

“…Evidence has shown that whole plant extracts and isolated natural compounds possess potent biological effects, including cytoprotective, antioxidant, anti-inflammatory, anti-thrombotic, anti-mutagenic and tumor inhibitory effects. There are many reports highlighting the ability of several whole plant extracts, vegetables, teas and fruits, as well as isolated phytochemicals to activate Nrf2, modulates antioxidant response and attenuate the NF-κβ pathway [ 13 , 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Red Palm Oil Ameliorates Oxidative Challenge and Inflammatory Responses Associated with Lipopolysaccharide-Induced Hepatic Injury by Modulating NF-κβ and Nrf2/GCL/HO-1 Signaling Pathways in Rats

et al. 2022

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Lipopolysaccharide (LPS), a well-conserved cell wall component of Gram positive bacteria, exerts its toxic effects via inducing oxidative and pro-inflammatory responses. Red palm oil (RPO) is a unique natural product with a balanced ratio of saturated and unsaturated fatty acids, with reported antioxidant and anti-inflammatory effects. In this study, we assess the protective effect and mechanistic action of RPO using a lipopolysaccharide (LPS)-induced hepatic injury model. Male Wistar rats were assigned into four groups (10 animals/group): normal control (NC), RPO, LPS and RPO + LPS. Animals in the RPO and RPO + LPS groups were administered RPO (200 μL/day) for 28 days. On the 27th day of experiment, animals in LPS and RPO + LPS groups were injected with LPS (0.5 mg/kg body weight). Animals were sacrificed 24 h later, and blood and liver tissues harvested for biochemical and molecular analysis. RPO resolved hepatic histological dysfunction induced by LPS, and lowered alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ-glutamyl transferase activities in the serum. Hepatic malondialdehyde and conjugated dienes, as well as pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6 and TNFα were significantly diminished (p < 0.05) by RPO pre-treatment. Activity of hepatic antioxidant enzymes including superoxide dismutase, glutathione reductase, glutathione peroxidase, as well as glutathione redox status (GSH:GSSG), and markers of antioxidant capacity that decreased as a result of LPS injection were improved by RPO pre-treatment. Mechanistically, RPO up-regulated mRNA expression of redox sensitive transcription factor Nrf2 and its downstream targets GCL and HO-1, while also suppressing the expression of NFκβ and associated inflammatory protein, Iκβ kinase (IκKβ). In conclusion, this study highlights the ameliorating effects of RPO against LPS-induced hepatic injury and revealed the Nrf2/GCL/HO-1 and NFκβ signaling axis as potential contributing mechanisms.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Red Palm Oil Ameliorates Oxidative Challenge and Inflammatory Responses Associated with Lipopolysaccharide-Induced Hepatic Injury by Modulating NF-κβ and Nrf2/GCL/HO-1 Signaling Pathways in Rats

et al. 2022

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“…Our data indicate that DEP SRM 2975 from an industrial forklift engine induce significant cytotoxic, prooxidative and pro-inflammatory effects in both human skin fibroblasts and keratinocytes. The data also show that aqueous extracts of two Rooibos preparations; fermented and green rooibos, and Honeybush teas protect these cells from the pro-oxidant and pro-inflammatory effects of DEP (SRM 2975).The cytotoxicity, pro-oxidative and pro-inflammatory effects of DEP have been reported in several studies (Oluyede et al, 2021;Lawal et al, 2015;Frikke-Schmidt et al, 2011;and Tseng et al, 2015). Different studies have shown DEP to induce ROS production, leading to oxidative stress with resultant damage to macromolecules and decrease GSH/GSSG ratio (Forchhammer et al, 2012;and Lawal et al, 2019a).…”

Section: Discussionmentioning

confidence: 84%

The effects of Rooibos (Aspalathus linearis) and honeybush (Cyclopia species) on air pollutant chemicals-induced cytoxicity in human skin fibroblasts and keratinocytes

Lawal¹,

Lester²,

Marnewick³

2022

African Journal of Biological Sciences

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Diesel Exhaust Particles (DEP) have been reported to be mainly responsible for most of the toxic effects of air pollution probably via a mechanism that involves induction of oxidative stress and proinflammatory response. Rooibos (Aspalathus linearis) and Honeybush (Cyclopia species) are widely used due to their health promoting effects. This study examined the effect of Rooibos tea and Honeybush on the prooxidative and proinflammatory effects of standard reference material (SRM) 2975 DEP in human skin fibroblasts and immortalized keratinocyte cell lines. The cells were either exposed to different concentrations (5-50 g/ml) of DEP for 4 h or pre-treated with 40 and 60 g/ml of fermented (FR) or green (GR) Rooibos or fermented Honeybush (FH) for 6 h prior to 4 h exposure to 10 g/ml DEP. Exposure to DEP caused a significant decrease in cell viability, and increase in LDH leakage, ROS production, oxidative stress biomarkers and proinflammatory cytokines in both cell lines compared to control. However, the presence of the extracts significantly (p < 0.001) attenuate these effects. This study highlights the potential of FR, GR and FH in protecting against DEP-induced oxidative stress and proinflammatory effect in fibroblasts and keratinocytes probably.

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“…Naringenin (4′,5,7-trihydroxyflavanone, with the molecular formula C 15 H 12 O 5 and a molecular weight of 272.25 g/mol) is a plant bioflavonoid that is naturally found in citrus fruits (up to 10% of its dry weight) [ 4 ], it contains two benzene rings linked together with a heterocyclic pyrone ring [ 5 , 6 ]. Hesperidin (hesperetin 7-rutinoside), a flavanone glycoside, is isolated from citrus fruit with a content of about 3%, and has the molecular formula C 28 H 34 O 15 and a molecular weight of 610.56 g/mol [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Citrus Naringenin Increases Neuron Survival in Optic Nerve Crush Injury Model by Inhibiting JNK-JUN Pathway

Chen

Yang

et al. 2021

IJMS

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Traumatic nerve injury activates cell stress pathways, resulting in neuronal death and loss of vital neural functions. To date, there are no available neuroprotectants for the treatment of traumatic neural injuries. Here, we studied three important flavanones of citrus components, in vitro and in vivo, to reveal their roles in inhibiting the JNK (c-Jun N-terminal kinase)-JUN pathway and their neuroprotective effects in the optic nerve crush injury model, a kind of traumatic nerve injury in the central nervous system. Results showed that both neural injury in vivo and cell stress in vitro activated the JNK-JUN pathway and increased JUN phosphorylation. We also demonstrated that naringenin treatment completely inhibited stress-induced JUN phosphorylation in cultured cells, whereas nobiletin and hesperidin only partially inhibited JUN phosphorylation. Neuroprotection studies in optic nerve crush injury mouse models revealed that naringenin treatment increased the survival of retinal ganglion cells after traumatic optic nerve injury, while the other two components had no neuroprotective effect. The neuroprotection effect of naringenin was due to the inhibition of JUN phosphorylation in crush-injured retinal ganglion cells. Therefore, the citrus component naringenin provides neuroprotection through the inhibition of the JNK-JUN pathway by inhibiting JUN phosphorylation, indicating the potential application of citrus chemical components in the clinical therapy of traumatic optic nerve injuries.

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Biochemical and molecular effects of naringenin on the cardiovascular oxidative and pro-inflammatory effects of oral exposure to diesel exhaust particles in rats

Cited by 8 publications

References 84 publications

Red Palm Oil Ameliorates Oxidative Challenge and Inflammatory Responses Associated with Lipopolysaccharide-Induced Hepatic Injury by Modulating NF-κβ and Nrf2/GCL/HO-1 Signaling Pathways in Rats

Red Palm Oil Ameliorates Oxidative Challenge and Inflammatory Responses Associated with Lipopolysaccharide-Induced Hepatic Injury by Modulating NF-κβ and Nrf2/GCL/HO-1 Signaling Pathways in Rats

The effects of Rooibos (Aspalathus linearis) and honeybush (Cyclopia species) on air pollutant chemicals-induced cytoxicity in human skin fibroblasts and keratinocytes

Citrus Naringenin Increases Neuron Survival in Optic Nerve Crush Injury Model by Inhibiting JNK-JUN Pathway

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