Biochemical and Molecular Markers: Unraveling Their Potential Role in Screening Germplasm for Thermotolerance

Ismail, Ahmed; Mosa, Kareem A.; Ali, Muna A.; Helmy, Mohamed

doi:10.1002/9781119432401.ch4

Cited by 3 publications

(2 citation statements)

References 57 publications

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“…Germplasm resistant to pathogenic race(s) is an economic and sustainable way to maintain yield plateau (Mundt 2014, Bailey-Serres et al 2019. DNA sequence based technology is being widely followed to avoid hindrance while selecting genotypes based on phenotypic characters (Nadeem et al 2018) and protecting important germplasm (Ismail et al 2020).…”

Section: Resultsmentioning

confidence: 99%

A molecular clue towards fusarium wilt resistant gene in melon (Cucumis melo L.) germplasm

Patel,

Dhatt,

Sharma

et al. 2021

VegSci

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Fusarium wilt [Fusarium oxysporum f. sp. melonis (Fom)], is one of the most serious diseases of muskmelon in India. Fifty accessions of muskmelon and its relatives collected from diverse sources were subjected to molecular screening with Fom1 and Fom2 gene specific markers. Presence of Fom1 (R) gene was confirmed using RG-9-2 marker where as susceptible allele was confirmed with another set of primer S-TAG/GCC-470. Out of 50 germplasm lines, 16 lines were found to be positive. Among which, eight were homozygous and eight were heterozygous in nature. The S-TAG/GCC- 470 could only able to confirm susceptible allele in exotic lines but was failed to confirm the same in Indian accessions. While for Fom2 gene, a combination of R-408 (R allele) and S-342 (S allele) were used. Six genotypes contained homozygous resistant gene while only three lines showed heterozygous nature for Fom2 gene. Only two genotypes viz., SM-2012-12 and IC-267359 contained both the resistant genes, whereas Kajri Sel.1, IC-267375, MM-314 and MM- 4305 contained Fom1 gene. These results conclude the presence of novel resistant gene in Indian melon gene pool and there is an opportunity for developing new gene based markers for these novel genes which will be useful to strengthen the resistance against newly evolving pathogenic races.

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Section: Resultsmentioning

confidence: 99%

A molecular clue towards fusarium wilt resistant gene in melon (Cucumis melo L.) germplasm

Patel,

Dhatt,

Sharma

et al. 2021

VegSci

View full text Add to dashboard Cite

show abstract

“…The application and protection of biodiversity have become easier and more proficient with the utilization of biochemical (protein) and molecular (DNA) markers. Biochemical markers can reveal the polymorphism of sequences of specific proteins as well as indirectly identify polymorphism of the DNA sequences from which they are translated (Ismail et al, 2020). These markers are based on the change in the sequence of amino acids in a protein molecule (White et al, 2007;Yuasa and Umetsu, 2005).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of the Beet Distributed in Armenia Using Protein Markers

Aloyan,

Badalyan,

Melikyan

2023

OnLine Journal of Biological Sciences

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The genetic resources of plants and animals are the wealth of every country. They are a valuable starting material in the breeding processes of cultivated plants and farm animals. In order to improve the existing varieties of crops and obtain new, valuable varieties, it is very important to document, preserve and enrich their gene pool, while preserving local varieties and forms of crops, it is possible to carry out breeding works. From this point of view, the genetic characterization of beet population varieties cultivated in Armenia and their wild relatives using protein markers is up-to-date and has important scientific and practical significance. It is known that the degree of genetic diversity of a population can be estimated if the protein formulas and their frequency of occurrence in the population are known. The purpose of this research was to evaluate the genetic diversity of beet spread in Armenia, formed during the evolutionary development processes, to passport the wild species and population varieties of beet by using protein markers and offering them as starting breeding material. As a result, the preferred raw material forms were proposed in the breeding works in the direction of increasing yield, sugar level, and resistance to powdery mildew and Cercospora leaf spot diseases. Further cluster analysis will make it possible to select parental pairs. Future beet breeding efforts in the target direction must make use of the genetic and genomic resources available for efficient improvement.

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Response of the antioxidant system of wheat seedlings with different genotypes to exogenous prooxidants: the relationship with resistance to abiotic stressors

Yastreb,

Kokorev,

Makaova

et al. 2023

Ukr.Biochem.J.

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Oxidative stress is an important component of heat- and drought-induced damage in plants. However, information on the relationship between the resistance of cultivated plants with different genotypes to environmental factors and their ability to maintain a pro-/antioxidant balance remains contradictory. This study aimed to compare the growth responses and adaptation ability of the antioxidant system in different wheat cultivars to oxidative stress agents hydrogen peroxide and iron(II) sulfate. Etiolated seedlings of common winter wheat (Triticum aestivum L.) Antonivka and Tobak (heat- and drought-tolerant), and Avgustina and Doskonala (not resistant to heat and drought) were used for the study. Three-day-old etiolated seedlings were exposed to 50 mM H2O2 or 5 mM FeSO4 for one day. It was found that seedlings of Antonivka and Tobak cultivars treated with H2O2 or FeSO4 were able to maintain relatively intensive growth, accumulated significantly lower amounts of endogenous hydrogen peroxide and lipid peroxidation products, significantly increased anthocyanin content and had a higher activity of SOD and catalase as compared with non-resistant cultivars. The non-tolerant cultivars’ response to stress agents was only to increase proline content with a simultaneous decrease in SOD activity and anthocyanins content. The identified varietal markers of the antioxidant system adaptive strategy can be used to develop new approaches for screening wheat cultivars with cross-resistance to major abiotic stressors. Keywords: antioxidant system, drought resistance, ferrous sulphate, H2O2, heat tolerance, oxidative stress, Triticum aestivum seedlings

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Biochemical and Molecular Markers: Unraveling Their Potential Role in Screening Germplasm for Thermotolerance

Cited by 3 publications

References 57 publications

A molecular clue towards fusarium wilt resistant gene in melon (Cucumis melo L.) germplasm

A molecular clue towards fusarium wilt resistant gene in melon (Cucumis melo L.) germplasm

Genetic Diversity of the Beet Distributed in Armenia Using Protein Markers

Response of the antioxidant system of wheat seedlings with different genotypes to exogenous prooxidants: the relationship with resistance to abiotic stressors

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