Biochemical and molecular mechanisms of acaricide resistance in Dermanyssus gallinae populations from Turkey

Koç, Nafiye; İnak, Emre; Nalbantoğlu, Serpil; Alpkent, Yasin Nazım; Dermauw, Wannes; Leeuwen, Thomas Van

doi:10.1016/j.pestbp.2021.104985

Cited by 13 publications

(11 citation statements)

References 66 publications

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“…Although there are varying degrees of molecular data on COI, 16S, ITS, Tropomyosin, and EF-1α sequences of D. gallinae from different countries (Roy et al 2010;Çiloğlu et al 2020;Karp-Tatham et al 2020;Roy et al 2021;Koç et al 2022), no sequence data belonging to other mitochondrial genes have been available so far, excluding the full mitochondrial genome. Considering the significance of the identification of genetic diversity within and between species from various geographical locations, there was a gap in the literature for the other mtDNA sequences of PRM.…”

Section: Resultsmentioning

confidence: 99%

“…The variation was detected secondly high in atp6 followed by ND5 and ND4 (Table 1). Although this variation based on cytb sequences can still be considered low, the determination of less variation (0.4%) within COI sequences of Turkish PRM populations (Koç et al 2022), might be an indicator for its usefulness as a marker. Moreover, the genetic variation was higher (1.7%) when Turkish PRM sequences were compared to cytb sequence of a Chinese PRM population, suggesting the increasing variation by distance which is an important feature of a marker.…”

Section: Resultsmentioning

confidence: 99%

“…Several physical and biological control methods were employed for reducing infestation in layer farms (Mul et al 2009;Sparagano et al 2014), whereas, the main control of PRMs still heavily relies on chemical acaricides (Sparagano et al 2020). Unfortunately, the intensive and frequent use of acaricides has gradually led to the development of resistance, ultimately resulting in the failure of PRM control (Marangi et al 2008;Katsavou et al 2020;Koç et al 2022).…”

Section: Introductionmentioning

confidence: 99%

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Screening of avian pathogens and genetic analysis of several mitochondrial genes in Dermanyssus gallinae populations in Türkiye</p >

Koç-inak,

Demirci,

Özbakış-Beceriklisoy

et al. 2024

saa

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The poultry red mite, PRM, Dermanyssus gallinae (De Geer, 1778), is widely regarded as the primary ectoparasite affecting egg-laying hens around the world. Besides direct feeding damage, the transmission of avian pathogens by PRMs is also a significant concern. Therefore, we aimed to screen the presence of 14 common avian pathogens in 32 PRM populations from egg-laying hens in Türkiye. As a result, Salmonella enterica (n=3), Tsukamurella paurometabola (n=18), and Avian fowlpox virus (n=4) (3 of which were wild-type APV) were detected in tested PRM populations. The results showed that the prevalence of avian pathogens in Turkish PRM populations was low. Nevertheless, these findings suggested that PRM can harbor disease-causing agents and may act as a vector in the spread of these pathogens in farms. In addition, four mitochondrial genes (mtDNAs) of D. gallinae, namely ND4, ND5, cytb, and atp6, were amplified, and obtained sequences were submitted to the GenBank database for the first time, which will allow further analyses. The genetic variation within cytb sequences of Turkish PRMs was the highest (1.01%), whereas intra-specific genetic distance was 1.7% when compared to a Chinese population (only available cytb sequence in the GenBank), indicating increasing genetic differentiation by distance. Last, more sequences belonging to mitochondrial genes are required to reveal their potential as molecular markers.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Screening of avian pathogens and genetic analysis of several mitochondrial genes in Dermanyssus gallinae populations in Türkiye</p >

Koç-inak,

Demirci,

Özbakış-Beceriklisoy

et al. 2024

saa

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“…Acaricide resistance is not unique to T . urticae , and other mite species, including Dermanyssus gallinae , Panonychus citri , Panonychus ulmi , have demonstrated resistance to acaricides through biochemical and molecular mechanisms of acaricide detoxification [ 14 , 15 ]. One of the most important mechanisms for acaricide resistance development in T .…”

Section: Introductionmentioning

confidence: 99%

“…Acaricide resistance can develop through multiple mechanisms, including enhanced metabolic breakdown of acaricides, target site insensitivity, and behavioral resistance [12,13]. Acaricide resistance is not unique to T. urticae, and other mite species, including Dermanyssus gallinae, Panonychus citri, Panonychus ulmi, have demonstrated resistance to acaricides through biochemical and molecular mechanisms of acaricide detoxification [14,15]. One of the most important mechanisms for acaricide resistance development in T. urticae is target site insensitivity, where single nucleotide polymorphisms (SNPs) can result in an alteration of an amino acid sequence and the translated protein which, in-turn, binds the insecticide more weakly or not at all [13].…”

Section: Introductionmentioning

confidence: 99%

Using targeted sequencing and TaqMan approaches to detect acaricide (bifenthrin, bifenazate, and etoxazole) resistance associated SNPs in Tetranychus urticae collected from peppermint fields and hop yards

et al. 2023

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Tetranychus urticae (Koch) is an economically important pest of many agricultural commodities world-wide. Multiple acaricides, including bifenazate, bifenthrin, and extoxazole, are currently registered to control T. urticae. However, populations of T. urticae in many different growing regions have developed acaricide resistance through multiple mechanisms. Within T. urticae, single nucleotide polymorphisms (SNPs) have been documented in different genes which are associated with acaricide resistance phenotypes. The detection of these mutations through TaqMan qPCR has been suggested as a practical, quick, and reliable tool to inform agricultural producers of acaricide resistance phenotypes present within their fields and have potential utility for making appropriate acaricide application and integrated pest management decisions. Within this investigation we examined the use of a TaqMan qPCR-based approach to determine genotypes which have been previously associated with acaricide resistance in field-collected populations of T. urticae from peppermint fields and hop yards in the Pacific Northwest of the United States and confirmed the results with a multiplex targeted sequencing. The results suggest that a TaqMan qPCR approach accurately genotypes T. urticae populations for SNPs that have been linked to Bifenazate, Bifenthrin, and Etoxazole resistance. The results also demonstrated that different populations of mites in Washington and Idaho displayed varying frequencies of the examined SNPs. While we were able to detect the SNPs associated with the examined acaricides, the mutation G126S was not an appropriate or accurate indicator for bifenazate resistance.

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Point mutations in the voltage‐gated sodium channel gene conferring pyrethroid resistance in China populations of the Dermanyssus gallinae

Wang,

Liu,

Wang

et al. 2024

Pest Management Science

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BACKGROUNDDermanyssus gallinae, the poultry red mite (PRM), is a worldwide ectoparasite posing significant economic challenges in poultry farming. The extensive use of pyrethroids for PRM control has led to the emergence of pyrethroid resistance. The objective of this study is to detect the pyrethroid resistance and explore its associated point mutations in the voltage‐gated sodium channel (VGSC) gene among PRM populations in China.RESULTSSeveral populations of D. gallinae, namely CJF‐1, CJP‐2, CJP‐3, CSD‐4 and CLD‐5, displayed varying degrees of resistance to beta‐cypermethrin compared to a susceptible field population (CBP‐5). Mutations of VGSC gene in populations of PRMs associated with pyrethroid resistance were identified through sequencing its fragments IIS4–IIS5 and IIIS6. The mutations I917V, M918T/L, A924G and L925V were present in multiple populations, while no mutations were found at positions T929, I936, F1534 and F1538.CONCLUSIONThe present study confirmed the presence of extremely high levels of pyrethroid resistance in PRM populations in China, and for the first time detected four pyrethroid resistance mutations in the VGSC gene. Identifying pyrethroid resistance in the field population of PRM in China can be achieved through screening for VGSC gene mutations as an early detection method. Our findings underscore the importance of implementing chemical PRM control strategies based on resistance evidence, while also considering the management of acaricide resistance in the control of PRMs. © 2024 Society of Chemical Industry.

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Biochemical and molecular mechanisms of acaricide resistance in Dermanyssus gallinae populations from Turkey

Cited by 13 publications

References 66 publications

Screening of avian pathogens and genetic analysis of several mitochondrial genes in Dermanyssus gallinae populations in Türkiye</p >

Screening of avian pathogens and genetic analysis of several mitochondrial genes in Dermanyssus gallinae populations in Türkiye</p >

Using targeted sequencing and TaqMan approaches to detect acaricide (bifenthrin, bifenazate, and etoxazole) resistance associated SNPs in Tetranychus urticae collected from peppermint fields and hop yards

Point mutations in the voltage‐gated sodium channel gene conferring pyrethroid resistance in China populations of the Dermanyssus gallinae

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