Biochemical and morphological correlations in human gallbladder with reference to membrane permeability

Hopwood, David A.; Ross, P. E.

doi:10.1002/(sici)1097-0029(19970915)38:6<631::aid-jemt7>3.0.co;2-h

Cited by 13 publications

(6 citation statements)

References 111 publications

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“…However, despite the fact that (in two case reports) it was speculated that the origin of such tumors might be Cajallike cells (Ortiz-Hidalgo et al 2000;Furihata et al 2005), the presence of interstitial Cajal-like cell type (ICLC) in a human non-tumoral gallbladder has not yet been thoroughly documented. It appears obvious why for ethical reasons, literary accounts of human material have only been from patients with chronic cholecystitis (Hopwood and Ross 1997). Another line of evidence has been provided by a recent report about the presence of cells similar to interstitial cells of Cajal in CD1 mice (Sun et al 2006) or guinea pig (Lavoie et al 2007) gallbladders, respectively.…”

Section: Introductionmentioning

confidence: 99%

Interstitial Cajal-like cells in human gallbladder

et al. 2007

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We describe here an interstitial Cajal-like cell type (ICLC) in human gallbladder, resembling the archetypal enteric interstitial cells of Cajal. Gallbladder ICLC were demonstrated in fresh preparations (tissue cryosections) using methylene-blue, and fixed specimens in Epon semi-thin sections stained with toluidine blue or transmission electron microscopy (TEM). The positive diagnosis of gallbladder ICLC was further verified by immunohistochemistry: CD117/c-kit, CD34, and another 16 antigens: vimentin, desmin, nestin, alpha-smooth muscle actin, NK-1, S-100, PGP-9.5, tau protein, chromogranin A, NSE, GFAP, CD1a, CD62-P, CD68, estrogen and progesterone receptors. Double immunostaining was performed for CD117, CD34 and CD117 and nestin, respectively. In fresh specimens, the spatial density of gallbladder ICLC was 100-110 cells/mm(2). ICLC mainly appeared beneath the epithelium and in muscularis (about 7%, and approximately 5%, respectively). In toto, ICLC represent in gallbladder approximately 5.5% of subepithelial cells. TEM showed that diagnostic criteria were fulfilled by ICLC. Moreover, TEM indicated that the main ultrastructural distinctive feature for ICLC, the cell processes, develop into the characteristic shape at a relatively early stage of development. It remains to be established if, in humans, ICLC are involved in gallbladder (dis)functions (e.g. pace-making, secretion (auto-, juxta- and/or paracrine), intercellular signaling, or stone formation).

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Section: Introductionmentioning

confidence: 99%

Interstitial Cajal-like cells in human gallbladder

et al. 2007

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“…11 In addition, more concentrated bile may affect gallbladder motility by increasing the movement of biliary compounds into gallbladder epithelial cells, by increasing the permeability of the gallbladder epithelium to cholesterol, and by favoring its accumulation in muscle membranes, thus reducing contraction. 13 Increased concentration of secondary BS may alter phospholipid acyl groups and, therefore, cholesterol solubility within the micelle/vesicle. 13 Finally, higher BS concentrations reaching the gut lumen may perturb the microbiota and, as a consequence, the enterohepatic BS circulation.…”

Section: Gallstones: Bad Company For the Steatotic Livermentioning

confidence: 99%

Gallstones: Bad Company for the Steatotic Liver

Alvaro¹

2017

Gastroenterology

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“…7---9 Taking into account the fact that the mixed (bile acids-phospholipidcholesterol) micelles are not absorbed by the gallbladder mucosa, cholesterol can be absorbed as monomers or with phospholipid vesicles. 7---12,24,27--- 29 The solubility of anhydrous cholesterol monomers in water is 0.013 nmol/ml; in the intermicellar phase it is 0.260 nmol/ml, while in the phospholipid vesicles it is 5.5 mol/ml. 30---37 Therefore, it will be absorbed with the phospholipid vesicles to a greater degree (99.9%), in accordance with the solubility of the anhydrous cholesterol.…”

Section: Mechanism Of Gallbladder Bile Formationmentioning

confidence: 99%

The role of the gallbladder in humans

Turumin¹,

Shanturov

Turumina³

2013

Revista de Gastroenterología de México

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The basic function of the gallbladder in humans is one of protection. The accumulation of the primary bile acids (cholic acid and chenodeoxycholic acid) in the gallbladder reduces the formation of the secondary bile acids (deoxycholic acid and lithocholic acid), thus diminishing their concentration in the so-called gallbladder-independent enterohepatic circulation and protecting the liver, the stomach mucosa, the gallbladder, and the colon from their toxic hydrophobic effects. The presence or absence of the gallbladder in mammals is a determining factor in the synthesis of hydrophobic or hydrophilic bile acids. Because the gallbladder contracts 5-20 min after food is in the stomach and the "gastric chyme" moves from the stomach to the duodenum 1-3 h later, the function of the gallbladder bile in digestion may be insignificant. The aim of this article was to provide a detailed review of the role of the gallbladder and the mechanisms related to bile formation in humans.

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Biochemical and morphological correlations in human gallbladder with reference to membrane permeability

Cited by 13 publications

References 111 publications

Interstitial Cajal-like cells in human gallbladder

Interstitial Cajal-like cells in human gallbladder

Gallstones: Bad Company for the Steatotic Liver

The role of the gallbladder in humans

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