Biochemical and Physiological Study of the Rest Period in the Tubers of Solanum tuberosum

Appleman, C. O.

doi:10.1086/331774

Cited by 20 publications

(9 citation statements)

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“…Time course experiments were run at 00, 100, 200, and 300 with buds excised from freshly harvested tubers that had been stored for 1 …”

Section: Changes In Sugarsmentioning

confidence: 99%

“…At 10-3 M, GA3 slightly inhibited production of reducing (1,3,9,13,29,35,38,40). Application of gibberellic acid promotes sprouting of intact tubers during tuber development or after they are harvested as marketable (23,36,38 the level of enzymes associated with carbohydrate metabolism (4,19,25,32).…”

Section: Introductionmentioning

confidence: 95%

“…Certain chemical treatments, as well as wounding, can shorten or prolong the rest period of potato tubers (1,3,9,13,29,35,38,40). Application of gibberellic acid promotes sprouting of intact tubers during tuber development or after they are harvested as marketable (23,36,38).…”

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confidence: 99%

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Regulation of Bud Rest in Tubers of Potato, Solanum tuberosum L

Clegg

Rappaport

1970

Plant Physiol.

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The rest period of the potato tuber was studied in relation to certain biochemical changes that are induced by gibberellic acid (GA3). The concentration of reducing sugars in excised plugs with buds treated with 10-M GA3 decreased in the first 4 hours after treatment and then rapidly increased up to 70 hours. The pattern in control buds was similar, but the changes occurred more slowly. The response to GAs is temperature-dependent and is not limited to any particular tissue of the tuber. The concentration of reducing sugars in excised buds increased proportionally to the log of the concentration of GA3 in a range from 10-8 to 10-4 M.At 10-3 M, GA3 slightly inhibited production of reducing (1,3,9,13,29,35,38,40). Application of gibberellic acid promotes sprouting of intact tubers during tuber development or after they are harvested as marketable (23,36,38 the level of enzymes associated with carbohydrate metabolism (4,19,25,32). There is mounting evidence that the hormone can induce synthesis of several hydrolytic enzymes (6,14,20,49,50). Working with Avena fatua, Simpson and Naylor (44) noted that the activity of maltase was higher in imbibed nondormant seeds than in imbibed dormant seeds. Gibberellin A3 caused an increase in maltase in dormant seeds, with a resultant increase in sugar production. Moreover, whereas excised nondormant embryos would grow on a sugar-amino acid medium, dormant embryos would grow on this medium only if GA3 was present (45). Thus in A. fatua, GA3 appears to have dual functions-to induce in the aleurone layers synthesis of enzymes that hydrolyze carbohydrate reserves and to induce in the embryo enzymes that utilize the resulting substrates. Development of cell wall invertase in tissue slices of Helianthus tuberosa in response to GA3 was observed by Edelman and Hall (10, 11) and Bacon, MacDonald, and Knight (2). Palmer (34) reported similar results with root slices of Beta vulgaris L.The expectation that GA3 would promote protein synthesis in potato buds was heightened by the observation that GA3 accelerates synthesis of RNA and DNA in excised buds from resting potatoes (39,41,49). We have shown (7) that reducing sugar content of potato buds increased shortly after excision, and the increase was accelerated by treatment with the hormone. It was therefore of interest to explore certain biochemical responses that occur in potato buds shortly after excision and treatment with GA3. MATERIALS AND METHODSPLANT MATERIALS White Rose potatoes were harvested 100 to 120 days after planting at which time they were in deep rest. The tubers were washed by hand and placed in the dark at 200. They were used within 3 days after harvest and were cooled to 00 hr before an experiment was begun; thereafter all procedures associated with preparation of plugs were at this temperature. In most experiments plugs with an apical bud (eye) were excised with a potato "deeyer" (internal diameter 0.8 cm) and cut to a length of 0.5 cm (35). In some experiments, 0.2 cm x 0.2 cm plugs with a bud were used. Such...

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“…Time course experiments were run at 00, 100, 200, and 300 with buds excised from freshly harvested tubers that had been stored for 1 …”

Section: Changes In Sugarsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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Regulation of Bud Rest in Tubers of Potato, Solanum tuberosum L

Clegg

Rappaport

1970

Plant Physiol.

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“…In accordance with the disinfection requirements which have been in effect in the United States since January 1,1926, all narcissus bulbs imported into this country and also those entering into interstate commerce are subjected to this treatment unless, after requisite inspection, they have been found free from bulb-fly larvae and eelworms.…”

Section: Introductionmentioning

confidence: 99%

Effect of Hot-Water Treatment on the Carbohydrate Changes in Narcissus Bulbs During Storage

Hasselbring¹

1932

Plant Physiol.

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“…This condition in the springs of 1904, 1924, and 1926, following unusually mild winters, prevailed to the extent that there were many acres of fruit trees completely dormant or growing indifferently increased sugar, but rather to some effect of high temperature on the protoplasm. APPLEMAN (2) reports an increase in the reducing sugar content of potato tubers under the influence of low temperature. He compared two sets of tubers, one held constantly at a growing temperature, and the other at a variable low temperature.…”

Section: Introductionmentioning

confidence: 99%

Composition and Growth Initiation of Dormant Bartlett Pear Shoots as Influenced by Temperature

Gardner

1929

Plant Physiol.

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Biochemical and Physiological Study of the Rest Period in the Tubers of Solanum tuberosum

Cited by 20 publications

References 0 publications

Regulation of Bud Rest in Tubers of Potato, Solanum tuberosum L

Regulation of Bud Rest in Tubers of Potato, Solanum tuberosum L

Effect of Hot-Water Treatment on the Carbohydrate Changes in Narcissus Bulbs During Storage

Composition and Growth Initiation of Dormant Bartlett Pear Shoots as Influenced by Temperature

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