Biochemical and structural basis of sialic acid utilization by gut microbes

Bell, Andrew; Severi, Emmanuele; Owen, C.D.; Latousakis, Dimitrios; Juge, Nathalie

doi:10.1016/j.jbc.2023.102989

Cited by 28 publications

(36 citation statements)

References 140 publications

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“…However, our own research did not uncover any evidence suggesting that fructose alone had a similar effect [42,43]. Exploring the role of sialic acid in relation to the gut microbiome, studies by North et al [44] and Bell et al [45] have highlighted its significance in the transportation mechanism of phosphate among various bacteria [46]. Interestingly, our findings indicate that there is no direct correlation between growth promotion within a phylum and its phylogenetic connection.…”

Section: Discussioncontrasting

confidence: 57%

Changes of microbiome in response to supplements with silver nanoparticles in cotton rhizosphere

Joshi,

Koradiya

et al. 2023

J Basic Microbiol

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The current study focuses on analyzing the effects of supplements containing silver nanoparticles (AgNPs) on plant growth and rhizospheric bacterial communities. Specifically, the impact of AgNP supplements was assessed on both plant growth promoting traits and bacterial communities in the soil. To do this, a screening process was conducted to select bacteria capable of synthesizing AgNPs through extracellular biosynthesis. UV‐Visible spectrophotometer, Fourier transform infrared, X‐ray diffraction, scanning electron microscope, and field emission scanning electron microscopy all confirmed, produced AgNPs is in agglomerates form. The resulting AgNPs were introduced into soil along with various supplements and their effects were evaluated after 10 days using next generation sequencing (Illumina—16S rDNA V3–V4 region dependent) to analyze changes in bacterial communities. Seed germination, root‐shoot biomass and chlorophyll content were used to assess the growth of the cotton plant, whereas the bacterial ability to promote growth was evaluated by measuring its culturable diversity including traits like phosphate solubilization and indole acetic acid production. The variance in Bray–Curtis β diversity among six selected combinations including control depends largely on the type of added supplements contributing to 95%–97% of it. Moreover, seed germination improves greatly between 63% and 100% at a concentration range of 1.4 to 2.8 mg/L with different types of supplements. Based on the results obtained through this study, it is evident that using AgNPs along with fructose could be an effective tool for promoting Gossypium hirsutum growth and enhancing plant growth traits like profiling rhizospheric bacteria. The results that have been obtained endorse the idea of boosting the growth of rhizospheric bacteria in a natural way when AgNPs are present. Using these supplements in fields that have been contaminated will lead to a better understanding of how ecological succession occurs among rhizospheric bacteria, and what effect it has on the growth of plants.

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Section: Discussioncontrasting

confidence: 57%

Changes of microbiome in response to supplements with silver nanoparticles in cotton rhizosphere

Joshi,

Koradiya

et al. 2023

J Basic Microbiol

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“…While free sialic acids are generally utilized as a nutrient by bacteria, sialic acids prevalently located at the termination of diverse glycoconjugates are acknowledged as the ligands for bacterial recognition and adhesion. , Ovomucin contains 2.6–8% sialic acids with α2,3- and α2,6-linkages; , ovomucin and its hydrolysates have been reported to interact with different microorganisms, where terminal sialic acid residues on the glycan structures play an important role. , Removing sialic acids from ovomucin and OP decreased their interactions with ETEC K88 (Table ); similarly, removing sialic acids from cell surfaces by NANase reduced ETEC K88 adhesion to IPEC-J2 and Caco-2 cells ( P = 0.0474 and 0.0048 by t test, respectively, Tables and S1). These findings were consistent with previous studies that sialic acids mediated the binding of enteric bacteria. ,, Free sialic acids decreased ETEC adhesion to Caco-2 cells (Table S1), aligning with previous research that sialic acids interfered with ETEC adhesion to Caco-2 cells .…”

Section: Discussionmentioning

confidence: 99%

Ovomucin Hydrolysates Reduce Bacterial Adhesion and Inflammation in Enterotoxigenic Escherichia coli (ETEC) K88-Challenged Intestinal Epithelial Cells

Bao,

Gänzle,

2024

J. Agric. Food Chem.

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Enterotoxigenic Escherichia coli (ETEC) K88 is the most common cause of diarrhea in neonatal and postweaning pigs. After adhering to small intestinal epithelial cells via glycoprotein receptor recognition, the pathogen can produce enterotoxins, impair intestinal integrity, trigger watery diarrhea, and induce inflammation via nuclear factor κB (NF-κB) and mitogen-activated protein kinase phosphatase (MAPK) pathways. Inhibiting ETEC K88 adhesion to cell surfaces by interfering with the receptorfimbriae recognition provides a promising strategy to prevent the initiation and progression of infection. Ovomucin is a highly glycosylated protein in chicken egg white with diverse bioactivities. Ovomucin hydrolysates prepared by the enzymes Protex 26L (OP) and pepsin/pancreatin (OPP) were previously revealed to prevent adhesion of ETEC K88 to IPEC-J2 cells. Herein, we investigated the protective effects of ovomucin hydrolysates on ETEC K88-induced barrier integrity damage and inflammation in IPEC-J2 and Caco-2 cells. Both hydrolysates inhibited ETEC K88 adhesion to cells and protected epithelial cell integrity by restoring transepithelial electronic resistance (TEER) values. Removing sialic acids in the hydrolysates reduced their antiadhesive capacities. Ovomucin hydrolysates suppressed ETEC-induced activation of NF-κB and MAPK signaling pathways in both cell lines. The ability of ETEC K88 in activating calcium/calmodulin-dependent protein kinase 2 (CaMK II), elevating intracellular Ca 2+ concentration, and inducing oxidative stress was attenuated by both hydrolysates. In conclusion, this study demonstrated the potential of ovomucin hydrolysates to prevent ETEC K88 adhesion and alleviate inflammation and oxidative stress in intestinal epithelial cells.

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“…However, it is usually found in the form of binding stage and at the end of N‐linked glycans or O‐linked glycans (Schauer & Kamerling, 2018). The N‐sugar chain contains a few to dozens of monosaccharides, including Sia, galactose (Gal), mannose (Man), fucose (Fuc), N ‐acetylglucosamine (GlcNAc), and N ‐acetylgalactosamine (GalNAc) (Bell et al., 2023). Sia bonds with Gal residues or GalNAc residues at the secondary end of the glycans via α‐2,3‐ or α‐2,6‐ linkages (Breg et al., 1987; Pfeiffer et al., 1992; Spik et al., 1988; Vliegenthart et al., 2010), or bonds with GlcNAc at the secondary end of the glycans via an α‐2,6‐ linkage (Mizuochi et al., 1979), or connects to other Sia residues via α‐2,8‐ or α‐2,9‐ linkages (Finne, 1985; Iwasaki et al., 1984).…”

Section: Neu5gcmentioning

confidence: 99%

N‐glycolylneuraminic acid in red meat and processed meat is a health concern: A review on the formation, health risk, and reduction

Liang,

Wu,

et al. 2024

Comp Rev Food Sci Food Safe

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One of the most consistent epidemiological associations between diet and human disease risk is the impact of consuming red meat and processed meat products. In recent years, the health concerns surrounding red meat and processed meat have gained worldwide attention. The fact that humans have lost the ability to synthesize N‐glycolylneuraminic acid (Neu5Gc) makes red meat and processed meat products the most important source of exogenous Neu5Gc for humans. As our research of Neu5Gc has increased, it has been discovered that Neu5Gc in red meat and processed meat is a key factor in many major diseases. Given the objective evidence of the harmful risk caused by Neu5Gc in red meat and processed meat to human health, there is a need for heightened attention in the field of food. This updated review has several Neu5Gc aspects given including biosynthetic pathway of Neu5Gc and its accumulation in the human body, the distribution of Neu5Gc in food, the methods for detecting Neu5Gc, and most importantly, a systematic review of the existing methods for reducing the content of Neu5Gc in red meat and processed meat. It also provides some insights into the current status and future directions in this area.

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Biochemical and structural basis of sialic acid utilization by gut microbes

Cited by 28 publications

References 140 publications

Changes of microbiome in response to supplements with silver nanoparticles in cotton rhizosphere

Changes of microbiome in response to supplements with silver nanoparticles in cotton rhizosphere

Ovomucin Hydrolysates Reduce Bacterial Adhesion and Inflammation in Enterotoxigenic Escherichia coli (ETEC) K88-Challenged Intestinal Epithelial Cells

N‐glycolylneuraminic acid in red meat and processed meat is a health concern: A review on the formation, health risk, and reduction

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