BIOCHEMICAL ASPECTS OF THE CELL GROWTH OF
            <i>ESCHERICHIA COLI</i>
            AS STUDIED BY THE METHOD OF SYNCHRONOUS CULTURE

Maruyama, Y

doi:10.1128/jb.72.6.821-826.1956

Cited by 48 publications

(12 citation statements)

References 12 publications

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“…Using mycobacteria, the highest protein concentration was found during the 2week period. Since protein was found to increase during the logarithmic phase of many other species of microorganisms (11,16,20), this supports the inference made from the RNA results that the cells were in the logarithmic phase at 2 weeks. Protein then gradually decreased about 50% through the fourth week, and this decrease paralleled the decrease of RNA during the same time period.…”

Section: Discussionsupporting

confidence: 81%

“…When mycobacterial RNA was used as a standard, however, the RNA results obtained with the attenuated H37Ra strain of M. tuberculosis were similar to the results found with several other microorganisms (3,4,6,7,12,15,16,18,20,22,24,26,27). The RNA content was highest with cells from 2-week-old pellicle cultures and decreased about 50% through the next 4 weeks.…”

Section: Discussionsupporting

confidence: 72%

“…The amount of DNA appeared to be highest just before or during the logarithmic phase of growth, again at 1 and 2 weeks, and then decreased by about 50% through the following 4 weeks. This has been shown to occur in other bacteria (2,16,18,24,27). However, some investigators have found DNA to remain fairly constant throughout the growth cycle (6, 7, 9, 12, 20).…”

Section: Discussionmentioning

confidence: 93%

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Ribonucleic Acid, Deoxyribonucleic Acid, and Protein Content of Cells of Different Ages ofMycobacterium tuberculosisand the Relationship to Immunogenicity

Youmans

1968

J Bacteriol

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The amount of ribonucleic acid (RNA), protein, and deoxyribonucleic acid (DNA) was determined in pellicle cultures of different ages of the H37Ra strain of Mycobccterium tuberculosis, grown on a synthetic medium. We found that the highest content of RNA and protein was present in 2-week-old cultures, indicating that these cells were in the logarithmic phase of growth. DNA content was highest at 1 and 2 weeks. The amount of all three compounds then decreased about 50% during the following 6 weeks. Two-week-old cells should therefore be used for preparation of the immunogenic ribosomal fraction. The optimal concentration of zinc chloride increased RNA and protein synthesis, and also improved the appearance of the pellicle growth. Two-week-old cells, which contained the largest amount of RNA and protein, immunized mice significantly better than older cells. Since protein and DNA are not involved in the production of immunity, a correlation could be made between amount of RNA and the capacity of viable H37Ra cells to immunize mice. The immunizing capacity of these cells was not affected by ribonuclease, probably because the ribonuclease did not penetrate into the whole cells.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 93%

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Ribonucleic Acid, Deoxyribonucleic Acid, and Protein Content of Cells of Different Ages ofMycobacterium tuberculosisand the Relationship to Immunogenicity

Youmans

1968

J Bacteriol

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“…On this model offspring resulting from a cell shocked during the phase of 'reproductive metabolism' may not be adapted to the environment in which they are produced, hence the high nonviability in the 2nd generation. Evidence of different metabolic states of the type suggested here is provided by the work of Bruce, Lark & Maaloe (1955), Delamaher (l956), Barner & Cohen (1956), Maruyama (1956), Burns (1959) and Maruysma & Lark (1961) among others, who have reported stepwise synthesis of deoxyribonucleic acid (DNA), ribonucleic acid (RNA) or protein. Contradictory evidence has been published by Abbo C % Pardee (1960), whose results suggest the continuous exponential synthesis of DNA, RNA, protein and induced P-galactosidase, whereas Young & Fitz-James (1959) found that during the growth cycle ".…”

Section: Viabilitymentioning

confidence: 66%

A Genealogical Study of Clonal Development of Escherichia coli

Quesnel

1963

Journal of Applied Bacteriology

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The generation times of individual Escherichia coli cells inoculated on to a cellophane membrane surrounded by liquid synthetic medium in a continuous flow system were determined by direct observation for up to seven generations. The presence and production of nonviable cells was also recorded. A wide variety of developmental patterns was found among the developing clones; increased viability and a decrease in generation times with increasing age of the population were .observed. The phenomenon of alternation of generations is described and discussed. Five factors contributory to the lag phase were distinguished, and their relative effect may be deduced from the results. I 28

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“…Some investigators find no evidence of disruption in the general interphase growth pattern during the time of cell division (Adolph and Bayne-Jones, 1932;Knaysi, 1940;Mitchison, 1957Mitchison, , 1958Abbo and Pardee, 1960;Williamson and Scopes, 1961). Other data, however, show marked reduction in growth rate during division (Bayne-Jones and Adolph, 1932;Knaysi, 1940;Zeuthen, 1953;Lindegren and Haddad, 1954;Prescott, 1955;Maruyama, 1956;Christensson, 1959;Hamburger and Zeuthen, 1960;Satir and Zeuthen, 1961) and, in some cases, there is evidence for a loss of mass at the time of division (Kimball, Caspersson, Svensson, and Carlson, 1959;Sandritter, Schiemer, Kraus, and D6rrien, 1960;Woodard, Gelber, and Swift, 1961).…”

Section: Introductionmentioning

confidence: 99%

Protein Synthesis and Rna Synthesis During Mitosis in Animal Cells

Konrad

1963

The Journal of Cell Biology

100

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Protcin synthesis and RNA synthcsis during mitosis wcrc studicd by autoradiography on mammalian tissue culture cells. Protein synthcsis was followed by incubating hamstcr epithelial and human amnion cclls for 10 or 15 minutes with phcnylalaninc-C TM. To study RNA synthcsis the hamster cells wcrc incubated for 10 minutes with uridinc-C 14. Comparisons of thc synthetic capacity of the intcrphasc and mitotic cells wcrc then madc using wholc ccll grain counts. The ratc of RNA synthcsis dccrcascd during prophasc and rcachcd a low of 13 to 16 pcr cent of the avcragc intcrphasc ratc during mctaphasc-anaphasc. Prorein synthcsis in thc hamstcr cells showed a 42 pcr cent incrcasc during prophasc with a subsequent rcturn to the avcragc interphasc valuc during mctaphasc-anaphasc. Thc human amnion cells showed no significant change at prophasc but thcrc was a 52 to 56 pcr ccnt drop in phcnylalanine incorporation at mctaphasc-anaphase as compared to the avcragc intcrphase rate. Colccmidc was used on thc hamster cells to study the cffcct of a prolongcd mitotic condition on protcin and RNA synthcsis. Undcr this condition, uridinc incorporation was extremely low whcrcas phenylalaninc incorporation was still rclativcly high. The drastic reduction of RNA synthesis observed under mitotic conditions is belicvcd to bc duc to the coiled condition of the chromosomes. The lack of a comparable reduction in protein synthcsis during mitosis is interprcted as evidence for the prcscnce in thcsc cclls of a rclatively stable mcsscngcr RNA.

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BIOCHEMICAL ASPECTS OF THE CELL GROWTH OF ESCHERICHIA COLI AS STUDIED BY THE METHOD OF SYNCHRONOUS CULTURE

Cited by 48 publications

References 12 publications

Ribonucleic Acid, Deoxyribonucleic Acid, and Protein Content of Cells of Different Ages ofMycobacterium tuberculosisand the Relationship to Immunogenicity

Ribonucleic Acid, Deoxyribonucleic Acid, and Protein Content of Cells of Different Ages ofMycobacterium tuberculosisand the Relationship to Immunogenicity

A Genealogical Study of Clonal Development of Escherichia coli

Protein Synthesis and Rna Synthesis During Mitosis in Animal Cells

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