Biochemical Assays in Spermatology

Srivastava, N. K.; Pande, Megha

doi:10.1007/978-981-10-5200-2_9

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Seminal initial fructose assessment was conducted immediately after collection (Srivastava and Pande, 2017b). An eosinaniline blue staining mixture was used to evaluate abnormal spermatozoa .…”

Section: Collection Of Datamentioning

confidence: 99%

Physiological Response, Semen Quality and Blood Biochemical Parameters of Rabbit Bucks Supplemented With Phytogenic Components During Summer Season of Egypt

Abdel-Azeem

Fayed

Azoz

2018

Egyptian Journal of Nutrition and Feeds

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eventy-two mature New Zealand White (NZW) rabbit's bucks were randomly and equally divided into eight groups each of nine bucks to evaluate the impact each of rocket seeds (RS), carrot seeds (CS), or bay laurel leaves (BLL) and their mixtures on semen quality, biochemical components and the physiological response of NZW rabbits bucks during hot summer conditions. The study started in June and lasted for 8 weeks. Eight experimental diets were formulated such that diet (D1) free additives as a control group. Diets 2, 3 and 4 contained 1.0% RS, 1.0% CS and 1.0% BLL, respectively. Diet 5, 6 and 7 contained a mix of 0.5% RS+0.5% CS, 0.5% CS+0.5% BLL and 0.5% RS+0.5% BLL, respectively, while diet 8 contained 0.33% RS+0.33% CS+0.33% BLL. Insignificant effect on buck's body weight, however, feed intake was significantly increased as compared to bucks group given the control diet. Supplementation with RS, CS, BLL, and their mixtures caused significant improvement in ejaculate volume, individual motility, total motile sperm, sperm concentration, live sperm%, total functional sperm fraction, total sperm output, and decreased abnormal sperm%. Seminal plasma initial fructose and globulin increased significantly with RS, CS, BLL, and their mixtures treatments. Seminal plasma alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) appeared reduction significantly with the RS, CS, BLL and their mixtures treatments. Seminal plasma total antioxidant capacity (TAC) increased, while, malondialdehyde (MDA) levels significantly decreased in all supplemented groups compared with the control bucks' value. Most of the supplemented groups showed a significant decrease in blood plasma glucose, cholesterol, triglycerides, and total lipids, low-density lipoproteins, AST and ALT as compared to the control group. However, blood plasma total protein and albumin were increased in comparison with the control group. Blood plasma TAC significantly increased due to the addition of RS, CS, BLL, and their mixtures as compared to the control group, but MDA levels decreased.

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Section: Collection Of Datamentioning

confidence: 99%

Physiological Response, Semen Quality and Blood Biochemical Parameters of Rabbit Bucks Supplemented With Phytogenic Components During Summer Season of Egypt

Abdel-Azeem

Fayed

Azoz

2018

Egyptian Journal of Nutrition and Feeds

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“…However, when the correlation analysis was applied in the samples with leukocytospermia ( n = 31), no significant correlation was noted between seminal ADA and SDFI, in spite of a significantly increased SDFI in this group. It may be assumed that in the presence of frank leukocytospermia, levels of various other chemical mediators are elevated and could have an additional role in causing damage to sperm DNA[2526] and thus leading to a lack of correlation between seminal ADA levels and SDFI.…”

Section: Discussionmentioning

confidence: 99%

Sperm DNA integrity in leukocytospermia and its association with seminal adenosine deaminase

et al. 2019

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Background:The study aimed to examine the effect of leukocytospermia on sperm quality and the levels of seminal adenosine deaminase (ADA) enzyme in males attending an infertility clinic in a tertiary hospital and to detect the association, if any, between seminal ADA and sperm quality.Methodology:Consenting male subjects, between 21 and 45 years, attending the infertility clinic and qualifying the eligibility criteria were recruited following informed consent. The collected semen samples were analyzed for the routine parameters based on the WHO protocols and for sperm DNA fragmentation. The seminal leukocyte count was detected using the peroxidase method, and the seminal ADA was assessed using spectrophotometry.Results:Samples from 110 participants were included in the study; leukocytospermia was detected in 33% of the samples. A significant reduction in the sperm quality with respect to conventional semen parameters (sperm motility and sperm vitality) and sperm DNA fragmentation index (SDFI) was noted in the presence of leukocytospermia. Furthermore, a significant positive correlation between the levels of seminal ADA and SDFI was noted (P = 0.000, r = 0.412).Conclusion:The sperm motility and DNA integrity are significantly compromised in the presence of leukocytospermia when the leukocyte count is > 1 million/mL of semen as well as 0.5–1 million/mL of semen. The positive correlation noted between seminal ADA levels and increased sperm DNA damage paves way for the possibility of seminal ADA to be an indicator of silent male genital tract inflammation as well as low-quality semen.

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Biochemical Assays in Spermatology

Cited by 2 publications

References 21 publications

Physiological Response, Semen Quality and Blood Biochemical Parameters of Rabbit Bucks Supplemented With Phytogenic Components During Summer Season of Egypt

Physiological Response, Semen Quality and Blood Biochemical Parameters of Rabbit Bucks Supplemented With Phytogenic Components During Summer Season of Egypt

Sperm DNA integrity in leukocytospermia and its association with seminal adenosine deaminase

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