Biochemical basis of the pharmacologic action of chondroitin sulfates on the osteoarticular system

Balì, Jean-Pierre; Cousse, Henri; Neuzil, E

doi:10.1053/sarh.2000.24874

Cited by 109 publications

(71 citation statements)

References 45 publications

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“…[4][5][6][7] This polysaccharide has recognized anti-inflammatory and antitumor activities and can be used in combination with other polysaccharides in different hydrogel drug delivery systems. 8,9 Another interesting sulfated polysaccharide is the sulfated fucan, which displays anticoagulant and antithrombotic activities. 1,10,11 Also known as fucoidan (FC), 12 this substance is found in brown algae (Fucus vesiculosus) and displays an ability to increase the inhibition of thrombin by antithrombin or heparin cofactor II.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Silva¹,

Garcia²,

Mori³

et al. 2012

IJN

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Abstract:The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Silva¹,

Garcia²,

Mori³

et al. 2012

IJN

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“…Chondroitin sulfates are sulfated linear polysaccharides comprising alternate uronic and N-acetyl-D-galactosamine residues. They are the predominant carbohydrate in proteoglycans produced by chondrocytes and monocytes/macrophages and are abundant in the extracellular matrix, accelerating the mineralization process and bone repair (23). Calcification and bone maturation are associated with a decrease in proteoglycan components of the matrix (24).…”

Section: Discussionmentioning

confidence: 99%

“…Calcification and bone maturation are associated with a decrease in proteoglycan components of the matrix (24). Physiologically, the chondroitin sulfates are bound to a core protein, which leads to aggregates of monomers with high molecular weights (23). This, along with the observations that OCIL has a higher binding capacity for the HMW sulfated GAGs than for the LMW sulfated GAGs and that the mOCIL lectin domain has a common binding site for recognition of both the LMW and HMW sugars, suggests that binding of the chondroitin sulfates by mOCIL may play an important physiological role in bone metabolism by possible interaction with these HMW aggregate sugars.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Sugar Binding by Osteoclast Inhibitory Lectin

Gange

Quinn

Zhou

et al. 2004

Journal of Biological Chemistry

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Osteoclast inhibitory lectin (OCIL) is a membranebound C-type lectin that blocks osteoclast differentiation and, via binding to its cognate receptor NKRP1D, inhibits natural killer cell-mediated cytotoxicity. OCIL is a member of the natural killer cell receptor C-type lectin group that includes CD69 and NKRP1D. We investigated carbohydrate binding of soluble recombinant human and mouse OCIL in enzyme-linked immunosorbent assay-based assays. OCIL bound immobilized high molecular weight sulfated glycosaminoglycans, including fucoidan, -carrageenan, and dextran sulfate, but not unsulfated dextran or sialated hyaluronic acid. Carbohydrate binding was Ca 2؉ -independent. Binding of immobilized low molecular weight glycosaminoglycans, including chondroitin sulfate (A, B, and C forms) and heparin, was not observed. However, the soluble forms of these low molecular weight glycosaminoglycans competed for OCIL binding of immobilized fucoidan (as did soluble fucoidan, dextran sulfate, and -carrageenan), indicating that OCIL does recognize these carbohydrates. Inhibition constants for chondroitin sulfate A and heparin binding were 380 and 5 nM, respectively. Immobilized and soluble monosaccharides did not bind OCIL. The presence of saturating levels of fucoidan, dextran sulfate, and -carrageenan did not affect OCIL inhibition of osteoclast formation. The fucoidan-binding lectins Ulex europaeus agglutinin I and Anguilla anguilla agglutinin did not block osteoclast formation or affect the inhibitory action of OCIL. Although the osteoclast inhibitory action of OCIL is independent of sugar recognition, we have found that OCIL, a lectin widely distributed, but notably localized in bone, skin, and other connective tissues, binds a range of physiologically important glycosaminoglycans, and this property may modulate OCIL actions upon other cells.

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“…Proteoglycans play a crucial role in intercellular communications that regulate important physiological processes, including growth factor retention, cellular adhesion/ proliferation support, differentiation induction, and provision of mechanical properties [27,28]. Because of its intrinsic stability and low immunogenicity in comparison with most ECM proteins, aggrecan (specifically CS) plays an important role in inflammation and immunity [29][30][31][32][33][34][35][36]. Both in vitro and in vivo studies have shown that CS itself regulates the formation of new cartilage by stimulating the chondrocyte synthesis of collagen, proteoglycans, and hyaluronan [37,38].…”

Section: Introductionmentioning

confidence: 99%

Chondroitin Sulfate Immobilized on a Biomimetic Scaffold Modulates Inflammation While Driving Chondrogenesis

Corradetti

Taraballi

Minardi

et al. 2016

Stem Cells Translational Medicine

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Biochemical basis of the pharmacologic action of chondroitin sulfates on the osteoarticular system

Cited by 109 publications

References 45 publications

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Characterization of Sugar Binding by Osteoclast Inhibitory Lectin

Chondroitin Sulfate Immobilized on a Biomimetic Scaffold Modulates Inflammation While Driving Chondrogenesis

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