Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds

Partington, L.; Mordan, NJ; Mason, Chris; Knowles, Jonathan C.; Hw, Kim; Lowdell, MW; Ma, Baohua; Wall, IB

doi:10.1016/j.actbio.2012.10.004

Cited by 104 publications

(94 citation statements)

References 39 publications

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“…The absence of GAGs reduces the restriction on water flow and causes a reduction in the water retention of the tissue, which also facilitates the diffusion of decellularisation solutions to remove soluble cellular components (Gilbert et al, 2006). The loss of GAGs has been associated with a reduction in the ultimate tensile strength of the tissue (Partington et al, 2013). However the biomechanical testing suggested this to have a minimal effect on the tissue (Table II).…”

Section: Resultsmentioning

confidence: 99%

Decellularisation and histological characterisation of porcine peripheral nerves

Žilić

Wilshaw

Haycock

2016

Biotech & Bioengineering

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Peripheral nerve injuries affect a large proportion of the global population, often causing significant morbidity and loss of function. Current treatment strategies include the use of implantable nerve guide conduits (NGC's) to direct regenerating axons between the proximal and distal ends of the nerve gap. However, NGC's are limited in their effectiveness at promoting regeneration Current NGCs are not suitable as substrates for supporting either neuronal or Schwann cell growth, as they lack an architecture similar to that of the native extracellular matrix (ECM) of the nerve. The aim of this study was to create an acellular porcine peripheral nerve using a novel decellularisation protocol, in order to eliminate the immunogenic cellular components of the tissue, while preserving the three‐dimensional histoarchitecture and ECM components. Porcine peripheral nerve (sciatic branches were decellularised using a low concentration (0.1%; w/v) sodium dodecyl sulphate in conjunction with hypotonic buffers and protease inhibitors, and then sterilised using 0.1% (v/v) peracetic acid. Quantitative and qualitative analysis revealed a ≥95% (w/w) reduction in DNA content as well as preservation of the nerve fascicles and connective tissue. Acellular nerves were shown to have retained key ECM components such as collagen, laminin and fibronectin. Slow strain rate to failure testing demonstrated the biomechanical properties of acellular nerves to be comparable to fresh controls. In conclusion, we report the production of a biocompatible, biomechanically functional acellular scaffold, which may have use in peripheral nerve repair. Biotechnol. Bioeng. 2016;113: 2041–2053. © 2016 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 99%

Decellularisation and histological characterisation of porcine peripheral nerves

Žilić

Wilshaw

Haycock

2016

Biotech & Bioengineering

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“…The presence of chondrocytes could be tolerated during the matrix remodelling and scaffold recellularization with the cells from the receptor animals 35 .…”

Section: Discussionmentioning

confidence: 99%

Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

et al. 2014

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PURPOSE:To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal. METHODS:This study was performed with New Zealand adult rabbits weighing 3.0 -4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase). RESULTS:Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present. CONCLUSION:The light-emitting diode is a promising tool for decellularization of soft tissues.

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“…26 On the other hand, processing of rat tracheal scaffolds using detergent-enzymatic treatments appeared to not influence the mechanical strength of the scaffold, despite alteration of extracellular matrix composition in vitro. 27 However, subsequent in vivo application of the decellularized rat trachea supported epithelilatizatin but failed to host engraftment of stem cell-derived chondrocytes.…”

Section: Matricesmentioning

confidence: 96%

Tissue Engineering of the Trachea: What is the Hold-up?

Siddiqi¹,

Wit²,

Timman³

et al. 2017

MOJCSR

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Tissue-engineering of the trachea is called for based on the clinical consequences of airway pathology. Recent years have witnessed various reports from the tissueengineering field including a few cases of clinical patients receiving an engineered airway. Multiple fields have accelerated in parallel with the concept of tissue decellularization, isolation of stem cells and possible insights in the biology of different stem cells. Yet, a clinical trial using a tissue-engineered trachea is yet to be initiated. What is the hold-up? Are the hurdles predominantly in the matrix field or cellular regeneration field or both? This systematic review is an attempt to evoke question on what the hold-up might be in the journey of successful regeneration of a functional airway.

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Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds

Cited by 104 publications

References 39 publications

Decellularisation and histological characterisation of porcine peripheral nerves

Decellularisation and histological characterisation of porcine peripheral nerves

Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

Tissue Engineering of the Trachea: What is the Hold-up?

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