Biochemical Changes in Blood of African Catfish (Clarias Gariepinus Burchell, 1822) during Artificial Spawning Using Different Doses of Human Chorionic Gonadotropin (HCG) Hormone

Abstract

This study was conducted to investigate the effect of different doses injection of human chorionic gonadotropin (HCG) hormone on blood biochemical parameters during artificial spawning of African catfish (Clarias gariepinus). African catfish spawners were intermuscularly injected with different doses of HCG (500, 1500, 3000, 6000 IU/kg female), and group is not injected as a control; males were injected at half the female dose. In the present study, who observed that hormonal injection by HCG hormone in male and female African catfish led to disturbance in all biochemical parameters such as serum total protein, glucose level, liver enzymes (AST and ALT activates), cholesterol, and (creatinine and urea) and cortisol concentrations. Serum total protein that recorded in female decreased with increasing HCG dose than that in the control. The lower glucose levels in female were observed with fish injected by 3000 and 6000 IU/kg female. African catfish male that injected with HCG were recorded higher levels of glucose than those obtained in the control group. The levels of serum glucose in male groups were higher than those recorded in female groups. Cholesterol levels were showed relatively decreased in male groups treated with HCG than that in the control group. The levels of ALT in fish that treat with HCG significantly increased than that the control group. Serum cortisol levels were increased in females and males after hormonal injection with HCG as compared to the control group. Under this conditions (the disturbance in biochemical parameters) tested in this study, the highest numbers of fertilized eggs/ female and fertilization rate, number of larvae/female and hatching rate were observed with 6000 IU/ kg female. While the incubation egg with 500 IU/kg female don't showed any hatching larvae. It was observed, increased in reproductive performance with the increase in HCG dosage.