Biochemical changes in skin and muscle after thermal injury

Lewis, G P; Löwe, Thomas; White, Alan M.; Worthington, Judy

doi:10.1042/bj1130014pa

Cited by 5 publications

(9 citation statements)

References 2 publications

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“…More importantly, in comparison with sCK activity, it has a clear advantage of being there as a stable form that can be monitored for 10 days. Since the increase in sLDH-5 isoenzyme persists longer than sCK, it may also have a role in the post-burn healing mechanism as a pyruvate oxidant (27). Similarly, the repair process of damaged tissue might have involvement by sLDH-3 which also shows an increase in its activity on the first day post--burn (Table I).…”

Section: Discussionmentioning

confidence: 99%

Observation on the Changes in Lactate Dehydrogenase Isoenzymes in Post-Burn Patients: Significance in Relation to Creatine Kinase

Ahmad¹,

Qayyum²,

Hasnain³

et al. 2009

Journal of Medical Biochemistry

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Observation on the Changes in Lactate Dehydrogenase Isoenzymes in Post-Burn Patients: Significance in Relation to Creatine Kinase The present study deals with the quantitative assessment of lactate dehydrogenase isoenzymes in the sera of burn subjects. Efforts are also made here to show better predictive marker value of sera LDH, as a few other known protein markers like creatine kinase and myoglobin have limited analytical value in the management of thermal burns. Blood was initially collected at day-1 of admission from 29 burn and 10 healthy subjects. Further, the sampling was carried out at 2, 5, 10, 20 and 30 days of wound healing (recovery). Plasma and sera LDH isoenzymes were monitored on 7.5% polyacrylamide gel electrophoresis. Quantitative assessment of LDH isoenzymes was done from gelscans using GelPro and Scion Imaging softwares. Sera CK levels were estimated colorimetrically using reagent kits. Our results show that quantitative changes in LDH isoenzyme were more convincing and interpretable in the sera than plasma. Sera LDH-5 isoenzyme was detected as the major contributor of total sera LDH activity, which follows a change parallel to sera CK in burn subjects. Sera LDH-5 activity also remains significantly high for up to 10 days while sera CK levels were detected elevated up to 5 days (P<0.05) during the recovery of patients. Therefore, the present findings strongly recommended the use of sera to assess the LDH activity and indicate better stability of sera LDH-5 than sera CK during post burn wound healing.

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Section: Discussionmentioning

confidence: 99%

Observation on the Changes in Lactate Dehydrogenase Isoenzymes in Post-Burn Patients: Significance in Relation to Creatine Kinase

Ahmad¹,

Qayyum²,

Hasnain³

et al. 2009

Journal of Medical Biochemistry

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“…In earlier investigations in cats (Lewis et al, 1970), it was concluded that the increase in the activity of lymph enzymes during the first 4 h after injury was not the result of leakage since tissues taken at 4 h already showed increased enzyme activities. It is possible therefore that this interpretation was incorrect since our experiments in rabbits are consistent with the view that enzyme protein leaves the tissue and passes to the lymph during the first few hours after injury.…”

Section: Glycogenmentioning

confidence: 99%

“…In cats Lewis et al (1970) showed that increases in the activity of several intracellular enzymes in the skin of the hind limb and the lymph draining the limb 4 h after a thermal injury were not influenced by prior administration of an inhibitor of protein synthesis, cycloheximide.…”

Section: Effect Of Cycloheximidementioning

confidence: 99%

“…Descriptions of the analysis and calculation of units of the following enzymes were given previously by Lewis et al (1970): lactate dehydrogenase (LDH) (L-lactate: NAD oxidoreductase, E.C.1.1.1.27); acid cathepsin (cathepsin D. E.C. …”

Section: Enzyme Analysismentioning

confidence: 99%

“…Lewis, Lowe, White & Worthington (1970) found that thermal injury to the hind limb of cats resulted in changes in the activity of several enzymes in the skin of the injured limb. When the injury was by heating at 800 C total acid phosphatase and g-glucuronidase activities fell significantly 4 h after injury whereas creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) activities were slightly, but not significantly, increased.…”

Section: Introductionmentioning

confidence: 99%

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Intracellular enzymes and protein synthesis in rabbit skin after thermal injury

Lewis¹,

Peters²,

White³

1971

British J Pharmacology

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Summary The concentrations of several intracellular enzymes in rabbit skin have been measured 5 min, 2, 6 and 24 h after thermal injury. At 5 min and 2 h after a burn (60° C for 1 min) there was a significant fall in the enzyme activities whereas at 6 h their activities were higher than control. It appears that the increase in enzyme concentrations in the lymph during the first few hours after thermal injury is associated with a fall in the enzyme concentrations in the tissues and therefore might be the result of leakage of enzyme from storage sites in the injured cells. The second increase in enzyme concentrations in the lymph which has been observed 6–18 h after thermal injury occurs at a time when there is also an increase in the enzyme concentrations in the tissue. It seems unlikely that these increased activities are due to new synthesis since there was no apparent correlation between tissue enzyme concentrations and protein synthetic activity, and the changes still occurred after administration of cycloheximide. There was a change in the LDH isoenzyme pattern after injury towards a predominance of LDH‐1. This change did not occur immediately after the burn, but was present at 2 and 6 h, and returned to normal 24 h later.

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Lymph flow and changes in intracellular enzymes during healing and rejection of rabbit skin grafts

Jasani¹,

Lewis²

1971

The Journal of Physiology

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SUMMARY1. Autografts and homografts of full thickness skin were made on a hind limb of rabbits. During the following days the appearance and histological changes of the grafts were studied; the lymph flow from the limb, and the enzyme activities in the supernatant and cell pellet of the lymph after centrifugation were determined, as well as the enzyme activities in the graft roof and the underlying host tissue. It was further examined whether a lymphatic and vascular connexion occurred between graft and host tissue.2. During the first 5 days the grafts changed from pale blue to bright pink, became swollen, soft and had a mild cellular inflammatory exudate. Autografts then became pale, took on the appearance of normal skin with the inflammatory changes subsiding, whereas homografts became firm, showed heavy mononuclear cell infiltration, had a blotchy purple appearance due to thrombosis and haemorrhage, developed widespread necrosis and changed into a black hard scab which was eventually shed. With high dose homografts (6-8 grafts) these changes occurred 1-2 days earlier than with low dose (2-4) grafts.3. The flow of lymph increased during the first 5 days after grafting, then returned to normal with autografts but remained increased with homografts.4. In the supernatant of the lymph the activities of LDH and figlucuronidase did not change during the first 5 days but activities of cathepsin, acid phosphatase, GOT and GPT increased. With the autografts the increase in the activities of these four enzymes then subsided, but with the homografts they increased further and there was an increase in the activities of LDH and /J-glucuronidase, even greater than in those of the other four enzymes.5. In the cell pellets of the lymph the activities of the six enzymes did not increase during the first 5 days; with homografts, but not with auto-M. K. JASANI AND G. P. LEWIS grafts, they then increased. These increases occurred even though the cell count in the pellet remained unchanged. Thus some of the lymphocytes must have become 'activated' to contain higher enzyme activities.6. The enzyme activities in the roof tissue did not parallel those in lymph. They did not change during the first three days. During the following three days the activities of acid phosphatase, LDH, /)-glucuronidase and cathepsin increased, but not those of GOT and GPT which remained low. From then onwards the behaviour was different with auto-and homografts. With autografts only the activity of acid phosphatase continued to increase, those of LDH, fl-glucuronidase and cathepsin decreased and those of GOT and GPT remained low. With homografts the activities of LDH, 8-glucuronidase and cathepsin continued to increase and became even greater than in the supernatant of lymph, whereas the activities of acid phosphatase, GOT and GPT, remained low.7. In the bed tissue the activities of all six enzymes increased during the first 3 days after grafting, then the activities of GOT and GPT returned towards normal but those of the other four increased further. The...

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Biochemical changes in skin and muscle after thermal injury

Cited by 5 publications

References 2 publications

Observation on the Changes in Lactate Dehydrogenase Isoenzymes in Post-Burn Patients: Significance in Relation to Creatine Kinase

Observation on the Changes in Lactate Dehydrogenase Isoenzymes in Post-Burn Patients: Significance in Relation to Creatine Kinase

Intracellular enzymes and protein synthesis in rabbit skin after thermal injury

Lymph flow and changes in intracellular enzymes during healing and rejection of rabbit skin grafts

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