Biochemical characteristics of fowl plague virus ts mutants

Ghendon, Y. Z.; Markushin, S. G.; Marchenko, A. T.; Sitnikov, B.S.; Ginzburg, V. P.

doi:10.1016/0042-6822(73)90170-0

Cited by 27 publications

(18 citation statements)

References 22 publications

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“…FPV Weybridge strain (H7N7) and ts mutants of this strain, ts 43, ts 166 and ts 29, were used. The origin and properties of these ts mutants have been described elsewhere Ghendon et al, 1973Ghendon et al, , 1975Ghendon et al, , 1982b. The ts 60 mutant was obtained by cultivation of FPV in chick embyro fibroblast (CEF) culture in the presence of N-methyl-N-nitro-N-nitrosoguanidine (5 ttg/ml) according to the method of Adachi et al (1980).…”

Section: Methodsmentioning

confidence: 99%

Studies of Fowl Plague Virus Temperature-sensitive Mutants with Defects in Synthesis of Virion RNA

Markushin¹,

Ghendon²

1984

Journal of General Virology

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Section: Methodsmentioning

confidence: 99%

Studies of Fowl Plague Virus Temperature-sensitive Mutants with Defects in Synthesis of Virion RNA

Markushin¹,

Ghendon²

1984

Journal of General Virology

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“…This cloning method pemnitted a massive and homogeneous production of single stranded DNA probes complementary to the minus and plus viral RNA. A large number of studies with temperaturesensitive mutants of Influenza virus (7,8,9,10,11) have been undertaken to elucidate which polypeptides are involved in RNA synthesis. With exception of the study of one FpV ts mutant (12), none of these experiments examined independently the synthesis of the three viral RNAs.…”

Section: Introductionmentioning

confidence: 99%

Use of specific single stranded DNA probes cloned in M13 to study the RNA synthesis of four temperature-sensitive mutants of HK/68 influenza virus

Thierry

Danos

1982

Nucl Acids Res

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Specific single stranded DNA probes have been obtained for both influenza virion RNA (vRNA) and complementary RNA (cRNA) by cloning a hemagglutinin gene fragment in the single stranded DNA phage M13. These probes were used for hybridization with the total labeled RNA from cytoplasmic extracts of infected cells. MDCK cells were infected with temperature-sensitive mutants of Influenza HK/68 and the production of the virus specific RNA species was analysed at both permissive and restrictive temperatures. Results show that two NP mutants which undergo intracistronic complementation exhibit two different phenotypes at the non permissive temperature: ts2C is poly A cRNA and vRNA negative whereas ts463 is RNA positive. Two mutants of P genes were also analysed and we discuss the relationship existing between the synthesis of the three RNA species especially between poly A and non poly A cRNA.

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“…Infected cells (Ioo EIDso/cell) were incubated in amino acid-free Eagle's medium at 36 °C for 60 min. Then hydrolysate of Chlorella 14C-proteins (2/zCi/ml) was added and cells were incubated at 36 °C for 5 h. The medium was then removed, warm (36 °C) Parker medium added and incubation continued at 36 °C for 17 h. Virus was sedimented from culture medium by centrifugation, purified (Klimov & Ghendon,I975) and studied electrophoretically in SDS-PAGE as described elsewhere (Ghendon et al 1973).…”

Section: Viruses Influenza Strains A/nws-d(hon0 A/ws-mk(honi)mentioning

confidence: 99%

“…This material was then treated with nuclease S-I (IOOO units/ml, 37 °C, 3 h), precipitated with ethanol and analysed by * Cells were infected with purified, aH-uridine-labelled virus and incubated at 36 °C for 5 h. RNAs were isolated, hybridized at 60 °C for 72 h and treated with RNase (a mixture of pancreatic and T-I RNases, final concentrations IO #g/ml and 2o units/ml, respectively; 37 °C, 30 min (for details see Ghendon Then a hydrolysate of *4C-proteins of Chlorella (I #Ci/ml) was added and incubation was continued for 60 min. Analysis of the virusspecific polypeptides synthesized was performed in cylindrical 7"5 ~ polyacrylamide gels containing SDS, as described previously (Ghendon et al 1973 …”

Section: Viruses Influenza Strains A/nws-d(hon0 A/ws-mk(honi)mentioning

confidence: 99%

Replication of Two Influenza Virus Strains and a Recombinant in HEF and LEP Cells

Ghendon

Tučková²,

Vonka³

et al. 1979

Journal of General Virology

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SUMMARYThe replication of influenza viruses A/NWS-D, A/WS-MK and their rl~ recombinant in human embryo fibroblast (HEF) and human diploid fibroblast (LEP) cell lines was studied. In HEF cells virus NWS-D and recombinant r12 induced synthesis of virus-specific macromolecules and produced infectious virions; virus WS-MK induced synthesis of virus complementary RNA (cRNA), virion RNA (vRNA), proteins, RNP and non-infectious virions, but haemagglutinin cleavage was impaired and the virions formed contained uncleaved haemagglutinin. In LEP cells, infectious virions were formed only by virus NWS-D; viruses WS-MK and r~2 induced synthesis of virus cRNA, vRNA, proteins and RNP; virus rlz had the haemagglutinin cleaved, whereas in virus WS-MK this process was impaired; neither virus WS-MK nor r~2 was capable of forming virions.

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Biochemical characteristics of fowl plague virus ts mutants

Cited by 27 publications

References 22 publications

Studies of Fowl Plague Virus Temperature-sensitive Mutants with Defects in Synthesis of Virion RNA

Studies of Fowl Plague Virus Temperature-sensitive Mutants with Defects in Synthesis of Virion RNA

Use of specific single stranded DNA probes cloned in M13 to study the RNA synthesis of four temperature-sensitive mutants of HK/68 influenza virus

Replication of Two Influenza Virus Strains and a Recombinant in HEF and LEP Cells

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