Biochemical characterization and application of a new lipase and its cognate foldase obtained from a metagenomic library derived from fat-contaminated soil
Lipases are very versatile enzymes, and produced the attention of the several industrial processes. Lipase can be achieved from several sources, animal, vegetable, and microbiological. The uses of microbial lipase market is estimated to be USD 425.0 Million in 2018 and it is projected to reach USD 590.2 Million by 2023, growing at a CAGR of 6.8% from 2018. Microbial lipases (EC 3.1.1.3) catalyze the hydrolysis of long chain triglycerides. The microbial origins of lipase enzymes are logically dynamic and proficient also have an extensive range of industrial uses with the manufacturing of altered molecules. The unique lipase (triacylglycerol acyl hydrolase) enzymes catalyzed the hydrolysis, esterification and alcoholysis reactions. Immobilization has made the use of microbial lipases accomplish its best performance and hence suitable for several reactions and need to enhance aroma to the immobilization processes. Immobilized enzymes depend on the immobilization technique and the carrier type. The choice of the carrier concerns usually the biocompatibility, chemical and thermal stability, and insolubility under reaction conditions, capability of easy rejuvenation and reusability, as well as cost proficiency.
Bacillus
spp.,
Achromobacter
spp.,
Alcaligenes
spp.,
Arthrobacter
spp.,
Pseudomonos
spp., of bacteria and
Penicillium
spp.,
Fusarium
spp.,
Aspergillus
spp., of fungi are screened large scale for lipase production. Lipases as multipurpose biological catalyst has given a favorable vision in meeting the needs for several industries such as biodiesel, foods and drinks, leather, textile, detergents, pharmaceuticals and medicals. This review represents a discussion on microbial sources of lipases, immobilization methods increased productivity at market profitability and reduce logistical liability on the environment and user.
Lipases are very versatile enzymes, and produced the attention of the several industrial processes. Lipase can be achieved from several sources, animal, vegetable, and microbiological. The uses of microbial lipase market is estimated to be USD 425.0 Million in 2018 and it is projected to reach USD 590.2 Million by 2023, growing at a CAGR of 6.8% from 2018. Microbial lipases (EC 3.1.1.3) catalyze the hydrolysis of long chain triglycerides. The microbial origins of lipase enzymes are logically dynamic and proficient also have an extensive range of industrial uses with the manufacturing of altered molecules. The unique lipase (triacylglycerol acyl hydrolase) enzymes catalyzed the hydrolysis, esterification and alcoholysis reactions. Immobilization has made the use of microbial lipases accomplish its best performance and hence suitable for several reactions and need to enhance aroma to the immobilization processes. Immobilized enzymes depend on the immobilization technique and the carrier type. The choice of the carrier concerns usually the biocompatibility, chemical and thermal stability, and insolubility under reaction conditions, capability of easy rejuvenation and reusability, as well as cost proficiency.
Bacillus
spp.,
Achromobacter
spp.,
Alcaligenes
spp.,
Arthrobacter
spp.,
Pseudomonos
spp., of bacteria and
Penicillium
spp.,
Fusarium
spp.,
Aspergillus
spp., of fungi are screened large scale for lipase production. Lipases as multipurpose biological catalyst has given a favorable vision in meeting the needs for several industries such as biodiesel, foods and drinks, leather, textile, detergents, pharmaceuticals and medicals. This review represents a discussion on microbial sources of lipases, immobilization methods increased productivity at market profitability and reduce logistical liability on the environment and user.
“…Lipases have a great affinity with a wide range of substrates, high stability to temperature, pH, and organic solvents, and can be chemoselective, regioselective and enantioselective (BARROS; FLEURI; MACEDO, 2010; GOLUNSKI et al, 2016). Due to these features and the ability to catalyze hydrolysis and synthesis reactions, lipases have been used in several applications in the pharmaceutical, food, chemical and cosmetic industries, in the formulation of detergents and the production of biodiesel (ALMEIDA et al, 2019;BOROWIECKI;DRANKA, 2019;ĆOROVIĆ et al, 2020;JAHANGIRI et al, 2018;MURILLO et al, 2019;PHUKON et al, 2020). Lipases can be produced from microorganisms (bacteria or fungi), plants (mainly from seeds and latex), and animals (pancreatic, hepatic or gastric lipases).…”
Oily wastewater from agro-industries can cause severe environmental and public health damages if unproperly treated. The conventional methods have several limitations for the treatment of oily wastewaters since the oil fraction can cause pipe clogging and biomass washout during the biological step. Based on this, the use of lipases can increase the oil removal efficiency of the treatments, minimizing operational problems, and improving the performance of the biological process. Lipases can also be used for the valorization of the oily wastewaters, transforming these waste streams into valuable compounds, such as biodiesel, lubricants, antioxidant agents, etc. The use of wastes for the production of value-added compounds follows the circular economy approach, minimizing the generation of residues and potential environmental liabilities. Thus, this study aims to give a general overview of the main topics related to the treatment and valorization of oily wastewaters using lipases. The main characteristics of oily wastewaters from agro-industries are addressed, as well as the most used conventional techniques for their treatment. Some relevant information about the enzyme lipase is also discussed to give the reader a background about how these enzymes can be applied for the treatment and transformation of oily wastes.
“…Lipases have a great affinity with a wide range of substrates, high stability to temperature, pH, and organic solvents, and can be chemoselective, regioselective and enantioselective (BARROS; FLEURI; MACEDO, 2010; GOLUNSKI et al, 2016). Due to these features and the ability to catalyze hydrolysis and synthesis reactions, lipases have been used in several applications in the pharmaceutical, food, chemical and cosmetic industries, in the formulation of detergents and the production of biodiesel (ALMEIDA et al, 2019;BOROWIECKI;DRANKA, 2019;ĆOROVIĆ et al, 2020;JAHANGIRI et al, 2018;MURILLO et al, 2019;PHUKON et al, 2020).…”
Oily wastewater from agro-industries can cause severe environmental and public health damages if unproperly treated. The conventional methods have several limitations for the treatment of oily wastewaters since the oil fraction can cause pipe clogging and biomass washout during the biological step. Based on this, the use of lipases can increase the oil removal efficiency of the treatments, minimizing operational problems,and improving the performance of the biological process. Lipases can also be used for the valorization of the oily wastewaters, transforming these waste streams intovaluable compounds, such as biodiesel, lubricants, antioxidant agents, etc. The use of wastes for the production of value-added compounds followsthe circular economy approach, minimizing the generation of residues and potential environmental liabilities. Thus, this study aims to give a general overview of themain topics related to thetreatment and valorization of oily wastewaters using lipases. The main characteristics of oily wastewaters from agro-industries areaddressed, as well as the most used conventional techniques for their treatment. Some relevant information about the enzyme lipaseisalso discussed to give the reader a background about how these enzymes can be applied for the treatment and transformation of oily wastes.
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