Biochemical characterization and biological properties of mycelium extracts from Lepista sordida GMA-05 and Trametes hirsuta GMA-01: new mushroom strains isolated in Brazil

Aranha, Guilherme Mauro; Contato, Alex Graça; Salgado, José Carlos dos Santos; Oliveira, Tássio Brito de; Retamiro, Karina Miyuki; Ortolan, Guilherme Guimarães; Crevelin, Eduardo José; Nakamura, Celso Vataru; Moraes, Luiz Alberto Beraldo de; Peralta, Rosane Marina; Polizeli, Maria de Lourdes Teixeira de Moraes

doi:10.1007/s42770-021-00670-5

Cited by 6 publications

(2 citation statements)

References 39 publications

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“…The 50 % cytotoxic concentration (CC 50 ) was determined by logarithm regression analysis of the data. [43] Further the selectivity index (SI) was calculated as described by Rodrigues et al [13] The SI can be defined as the ratio of the toxic concentration (CC 50 ) by the effective bioactive concentration (IC 50 ) for the different Leishmania forms.…”

Section: Activity Against Intracellular Amastigotesmentioning

confidence: 99%

Antiproliferative Activity and Ultrastructural Changes in Promastigote and Amastigote forms of Leishmania amazonensis Caused by Limonene‐Acylthiosemicarbazide Hybrids

Contato

Kaplum

Scariot

et al. 2023

Chemistry & Biodiversity

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Leishmaniasis is a tropical zoonotic disease. It is found in 98 countries, with an estimated 1.3 million people being affected annually. During the life cycle, the Leishmania parasite alternates between promastigote and amastigote forms. The first line treatment for leishmaniasis are the pentavalent antimonials, such as N-methylglucamine antimoniate (Glucantime®) and sodium stibogluconate (Pentostam®). These drugs are commonly related to be associated with dangerous side effects such as cardiotoxicity, nephrotoxicity, hepatotoxicity, and pancreatitis. Considering these aspects, this work aimed to obtain a new series of limonene-acylthiosemicarbazides hybrids as an alternative for the treatment of leishmaniasis. For this, promastigotes, axenic amastigotes, and intracellular amasti-gotes of Leishmania amazonensis were used in the antiproliferative assay; J774-A1 macrophages for the cytotoxicity assay; and electron microscopy techniques were performed to analyze the morphology and ultrastructure of parasites. ATZÀ S-04 compound showed the best result in both tests. Its IC 50 , in promastigotes, axenic amastigotes and intracellular amastigotes was 0.35 � 0.08 μM, 0.49 � 0.06 μM, and 15.90 � 2.88 μM, respectively. Cytotoxicity assay determined a CC 50 of 16.10 � 1.76 μM for the same compound. By electron microscopy, it was observed that ATZÀ S-04 affected mainly the Golgi complex, in addition to morphological changes in promastigote forms of L. amazonensis.

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Section: Activity Against Intracellular Amastigotesmentioning

confidence: 99%

Antiproliferative Activity and Ultrastructural Changes in Promastigote and Amastigote forms of Leishmania amazonensis Caused by Limonene‐Acylthiosemicarbazide Hybrids

Contato

Kaplum

Scariot

et al. 2023

Chemistry & Biodiversity

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“…sordida is very worthy of research and development. In several investigations, several significant amounts of compounds were mentioned to be present in its fruiting body, such as phenols, flavonoids, and ascorbic acid, which presented excellent free radical scavenging potency showing potential as functional foods or additives (Aranha et al, 2022) [3] . Some new chlorinated sesquiterpenes and diterpenoids isolated from the mycelial culture broth of L. sordida could be used as inducers of the differentiation of human leukemic cells (Kang et al, 2017; Chen et al, 2011) [4] .…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomics analysis reveals the domesticated Lepista sordida primordium differentiation regulation mechanism and the subsequent different development patterns in the pileus and stipe

Yang,

Liu,

Zhang

et al. 2024

Food Science and Human Wellness

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The wild Lepista sordida is a kind of precious and rare edible fungus. An excellent strain of it by artificial domestication was obtained, which was high-yield and high in iron content. In this study, high-throughput comparative proteomics was used to reveal the regulatory mechanism of its primordium differentiation in the early fruiting body formation. The mycelium before the primordium differentiation mainly expressed high levels of mitochondrial functional proteins and carbon dioxide concentration regulatory proteins. In young mushrooms, the highly expressed proteins were mainly involved in cell component generation, cell proliferation, nitrogen compound metabolism, nucleotide metabolism, glutathione metabolism, and purine metabolism. The differential regulation patterns of pileus and stipe growth to maturity were also revealed. The highly expressed proteins related to transcription, RNA splicing, the production of various organelles, DNA conformational change, nucleosome organization, protein processing, maturation and transport, and cell detoxification regulated the pileus development and maturity. The proteins related to carbohydrate and energy metabolism, large amounts of obsolete cytoplasmic parts, nutrient deprivation, and external stimuli regulated the stipe development and maturity. Multiple Cazymes regulated nutrient absorption, morphogenesis, spore production, stress response, and other life activities at different growth and development stages.

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Trametes betulina (L.) Pilát.; Trametes cinnabarina (Jacq.) Fr.; Trametes gibbosa (Pers.) Fr.; Trametes hirsuta (Wulfen) Lloyd; Trametes pubescens (Schumach.) Pilát; Trametes suaveolens (L.) Fr.; Trametes versicolor (L.) Lloyd - POLYPORACEAE

Gafforov,

Rašeta,

İnci

et al. 2023

Ethnobiology

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Biochemical characterization and biological properties of mycelium extracts from Lepista sordida GMA-05 and Trametes hirsuta GMA-01: new mushroom strains isolated in Brazil

Cited by 6 publications

References 39 publications

Antiproliferative Activity and Ultrastructural Changes in Promastigote and Amastigote forms of Leishmania amazonensis Caused by Limonene‐Acylthiosemicarbazide Hybrids

Antiproliferative Activity and Ultrastructural Changes in Promastigote and Amastigote forms of Leishmania amazonensis Caused by Limonene‐Acylthiosemicarbazide Hybrids

Comparative proteomics analysis reveals the domesticated Lepista sordida primordium differentiation regulation mechanism and the subsequent different development patterns in the pileus and stipe

Trametes betulina (L.) Pilát.; Trametes cinnabarina (Jacq.) Fr.; Trametes gibbosa (Pers.) Fr.; Trametes hirsuta (Wulfen) Lloyd; Trametes pubescens (Schumach.) Pilát; Trametes suaveolens (L.) Fr.; Trametes versicolor (L.) Lloyd - POLYPORACEAE

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