Biochemical characterization and identification of differentially expressed candidate genes in salt stressed sugarcane

Pagariya, Madhuri Chandrakant; Devarumath, R. M.; Kawar, Prashant G.

doi:10.1016/j.plantsci.2011.12.002

Cited by 48 publications

(30 citation statements)

References 51 publications

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“…The Pro concentration supports its role as an antioxidant, while other amino acids act as osmoprotectant during sucrose accumulation in the culms. The accumulation of free amino acids is commonly found in plants under stresses (Patade et al, 2008; Pagariya et al, 2012), raising the osmotic pressure, and therefore, operating as an osmoregulator (Venekamp et al, 1987; Molinari et al, 2004; Boaretto et al, 2014). …”

Section: Ros Accumulation and Antioxidant Activitymentioning

confidence: 99%

Sugarcane Water Stress Tolerance Mechanisms and Its Implications on Developing Biotechnology Solutions

et al. 2017

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Sugarcane is a unique crop with the ability to accumulate high levels of sugar and is a commercially viable source of biomass for bioelectricity and second-generation bioethanol. Water deficit is the single largest abiotic stress affecting sugarcane productivity and the development of water use efficient and drought tolerant cultivars is an imperative for all major sugarcane producing countries. This review summarizes the physiological and molecular studies on water deficit stress in sugarcane, with the aim to help formulate more effective research strategies for advancing our knowledge on genes and mechanisms underpinning plant response to water stress. We also overview transgenic studies in sugarcane, with an emphasis on the potential strategies to develop superior sugarcane varieties that improve crop productivity in drought-prone environments.

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Section: Ros Accumulation and Antioxidant Activitymentioning

confidence: 99%

Sugarcane Water Stress Tolerance Mechanisms and Its Implications on Developing Biotechnology Solutions

et al. 2017

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“…They are found to be critical signalling components during stress response and activate cell death and defense responses [29]. An ankyrin-like protein was found up regulated in salt stressed sugarcane plants [30]. These proteins are associated with biotic and abiotic stress and stimulate downstream signal transduction.…”

Section: Discussionmentioning

confidence: 99%

Understanding sugarcane defence responses during the initial phase of Colletotrichum falcatum pathogenesis by suppression subtractive hybridization (SSH)

Sathyabhama

Viswanathan

Nandakumar

et al. 2015

Physiological and Molecular Plant Pathology

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“…POD activity was measured using the method of Pagariya et al (2012). CAT activity was determined as described by Tseng et al (2007).…”

Section: Methodsmentioning

confidence: 99%

VaERD15, a Transcription Factor Gene Associated with Cold-Tolerance in Chinese Wild Vitis amurensis

Zhang

Zhao

et al. 2017

Front. Plant Sci.

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Early responsive to dehydration (ERD) genes can be rapidly induced to counteract abiotic stresses, such as drought, low temperatures or high salinities. Here, we report on an ERD gene (VaERD15) related to cold tolerance from Chinese wild Vitis amurensis accession ‘Heilongjiang seedling’. The full-length VaERD15 cDNA is 685 bp, including a 66 bp 5′-untranslated region (UTR), a 196 bp 3′-UTR region and a 423 bp open reading frame encoding 140 amino acids. The VaERD15 protein shares a high amino acid sequence similarity with ERD15 of Arabidopsis thaliana. In our study, VaERD15 was shown to have a nucleic localization function and a transcriptional activation function. Semi-quantitative PCR and Western blot analyses showed that VaERD15 was constitutively expressed in young leaves, stems and roots of V. amurensis accession ‘Heilongjiang seedling’ plants, and expression levels increased after low-temperature treatment. We also generated a transgenic Arabidopsis Col-0 line that over-expressed VaERD15 and carried out a cold-treatment assay. Real-time quantitative PCR (qRT-PCR) and Western blot analyses showed that as the duration of cold treatment increased, the expression of both gene and protein levels increased continuously in the transgenic plants, while almost no expression was detected in the wild type Arabidopsis. Moreover, the plants that over-expressed VaERD15 showed higher cold tolerance and accumulation of proline, soluble sugars, proteins, malondialdehyde and three antioxidases (superoxide dismutase, peroxidase, and catalase). Lower levels of relative ion leakage also occurred under cold stress. Taken together, our results indicate that the transcription factor VaERD15 was induced by cold stress and was able to enhance cold tolerance.

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Biochemical characterization and identification of differentially expressed candidate genes in salt stressed sugarcane

Cited by 48 publications

References 51 publications

Sugarcane Water Stress Tolerance Mechanisms and Its Implications on Developing Biotechnology Solutions

Sugarcane Water Stress Tolerance Mechanisms and Its Implications on Developing Biotechnology Solutions

Understanding sugarcane defence responses during the initial phase of Colletotrichum falcatum pathogenesis by suppression subtractive hybridization (SSH)

VaERD15, a Transcription Factor Gene Associated with Cold-Tolerance in Chinese Wild Vitis amurensis

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