Biochemical Characterization and In Vitro Digestibility of Protein Isolates from Hemp (Cannabis sativa L.) By-Products for Salmonid Feed Applications

Banskota, Arjun H.; Tibbetts, Sean M.; Jones, Alysson; Stefanova, Roumiana; Behnke, Joerg

doi:10.3390/molecules27154794

Cited by 14 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The recovery of protein at the isoelectric point of the hemp seed was previously reported in several studies. The recovery of hemp seed protein at the isoelectric point (pH 4.5) was 84% by [ 24 ] and 50.60% of protein recovered by [ 22 ]. The differences in protein recovery might be referred to several factors such as the variety of the seeds, the protein–protein interaction that affected the level of protein recovery, and the isolation conditions as well.…”

Section: Resultsmentioning

confidence: 99%

“…The SDS–PAGE profile of the HPI is presented in Figure 1 c, d. The most predominant protein appeared in the band with high intensity and MW 58.1 KDa, which may be corresponding to edestin [ 27 ] followed by the protein with a molecular weight 31.5 KDa, which may be attributed to albumin [ 6 ] ( Figure 1 d. On the other hand, the HPI electrophoresis profile showed another four bands with moderate concentrations according to the intensity of the bands ( Figure 1 d) with the molecular weights 63.2 KDa, 53.2 kDa, 49.8 KDa and 41.4 kDa. Globulin protein is about 85% of total hemp proteins [ 28 ], which exhibited the lowest solubility at pH 5 [ 24 ]. The globulin fraction is made of 93% of 11 s legumin, which is called edestin [ 22 , 23 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Nutritional Quality, Chemical, and Functional Characteristics of Hemp (Cannabis sativa ssp. sativa) Protein Isolate

El-Sohaimy

Androsova

Toshev

et al. 2022

Plants

View full text Add to dashboard Cite

(1) Background: Hemp seeds are a source of plant-based protein, making them an appropriate supplement to a plant-based diet. The current work was focused on the preparation of the protein isolate from the hemp seeds with eco-friendly and cheap technology. Moreover, it evaluated the physicochemical and functional properties of hemp protein isolate for its potential application in food manufacturing. (2) Methods: The protein content of hemp seeds has been isolated through two main steps: (1) extraction of the protein content of an alkaline pH (10–12); (2) precipitation of the extracted protein on an acidic pH as an isoelectric point (pH = 4.5). (3) Results: The edastin protein is the most predominant protein in the protein profile with a molecular weight of 58.1 KDa beside albumin with a molecular weight of 31.5 KDa. The FTIR spectrum detected the absorption peaks of the amide I at 1750 and 1600 cm−1, which pointed to C=O stretching while N-H stretching at 1650–1580 cm−1. The peak at 3250 is found to be related to N-H stretching of the aliphatic primary amine (3400–3300 cm−1) and the N-H stretching for the secondary (II) amine appeared at 3350–3310 cm−1. The Hemp protein isolate (HPI) showed a high content of arginine (15.52 g/100 g), phenylalanine + tyrosine (9.63 g/100 g), methionine + cysteine (5.49 g/100 g), leucine + isoleucine (5.21 g/100 g), and valine (4.53 g/100 g). It contains a moderate level of threonine (3.29 g/100 g) and lysine (2.50 g/100 g) with tryptophan as the limiting amino acid (0.22 g/100 g). The HPI showed an appropriate water-and-oil holding capacity (4.5 ± 2.95 and 2.33 ± 1.88 mL/g, respectively). The foaming capacity of the HPI was increased with increasing the pH values to reach the maximum value at pH 11 (67.23 ± 3.20%). The highest emulsion ability index of the HPI was noted at pH 9 (91.3 ± 2.57 m2/g) with low stability (19.15 ± 2.03). (4) Conclusion: A strong positive correlation (r = 0.623) was shown between protein concentration and solubility. The current easy-to-use, cheap, and eco-friendly technology provides the industrial sector with a cheap protein isolate for manufacturing protein-rich diet and beverages. The HPI showed a good nutritional quality and functional properties that might be helpful in utilizing it in different food products such as beverages and bakery products.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Nutritional Quality, Chemical, and Functional Characteristics of Hemp (Cannabis sativa ssp. sativa) Protein Isolate

El-Sohaimy

Androsova

Toshev

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

“…The main factors that indicate the quality of a protein are their bioavailability and digestibility. A number of authors studied the amino acid profile of hemp (Cannabis sativa L.) protein [46,47]. The analyzed product was the protein extracted from whole hemp (Cannabis sativa L.) seeds, and the protein isolate from commercial hemp, so that the obtained results are expressed either in regards to the whole seeds (g of amino acids per 100 g of seeds), or in regards to the total proteins (g of amino acids per 100 g of protein) [33].…”

Section: Protein Contentmentioning

confidence: 99%

Hemp (<i>Cannabis sativa</i> L.) seeds nutritional aspects and food production perspectives: A review

Capcanari,

Covaliov,

Negoița

2024

Food systems

View full text Add to dashboard Cite

This review is devoted to an analysis of the hemp (Cannabis sativa L.) seeds’ nutritional aspects and food production perspectives, that can become a valuable source of multifunctional components for functional food production. Cannabis sativa L. is a multipurpose crop with lowenvironmental impact traditionally cultivated in Western cultures for fiber production. The propagation of synthetic fibers and the production of intoxicating drugs from certain narcotic strains resulted in the banning of its cultivation. Thus, culturing the varieties that are widely known as „industrial hemp“ has only been practiced for the past two decades. Hemp (Cannabis sativa L.) is grown not only for its economic importance but also for the seeds’ nutritional value. Hemp seeds consists of 25–35% lipids with perfectly balanced fatty acids (FAs); 20–25% proteins, which are easy to digest and contain all essential amino acids; 20–30% carbohydrates, mainly dietary fiber; and vitamins and minerals. Besides its nutritional value, hempseed is also rich in antioxidants and bioactive compounds such as bioactive peptides, polyphenols with high free radicals scavenging activity, and cannabinoids. Therefore, this study reviews the scientific knowledge about Cannabis sativa L. seeds and their progressive aspects of cultivation, functional and therapeutic potential, and its use in functional food production.

show abstract

“…Amino acid profiling analysis was conducted according to Banskota et al using the AccQTag Ultra (Waters, Milford MA, USA) method. 47,48 Briefly, 10 mg sample containing internal standard ⊍-aminobutyric acid (ABA) was hydrolyzed with 1 mL of constant boiling HCl (∼12 mol L −1 ) at 120 °C for 24 h in triplicate. The reaction was cooled to RT, filtered through cotton, and diluted to 5 mL with Milli-Q water.…”

Section: Biochemical Composition Analysis Of Biomassmentioning

confidence: 99%

Phycoremediation and simultaneous production of protein‐rich algal biomass from aquaculture and agriculture wastewaters

Bhatti

Richards

Wall

et al. 2023

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Microalgae cultivation in wastewaters is a potential remediation technology for converting residual nutrients into valuable biomass for biofuels, fertilizers, animal and aquaculture feeds, and bio-based chemicals. The objective of this study was to determine the potential for microalgae phycoremediation of aquaculture and agriculture wastewaters for sustainable production of nutrient-rich algal biomass.RESULTS: Thirty-seven aquaculture and agriculture wastewaters were evaluated as growth media for two freshwater, chlorophytic microalgae strains (Chlorella sorokiniana SMC14M and Scenedesmus sp. AMDD). Although nutrient levels and physicochemical parameters of the wastewaters varied widely, phycoremediation efficiency of nitrogen and phosphorous was high (> 70%) for a large majority of them, with both microalgae strains. Photobioreactor cultivation (300 L) of C. sorokiniana grown on finfish aquaculture wastewater (CA2) and standard growth medium (SGM) exhibited similar biomass production levels and nutritional compositions of moisture (4.9-5.0%), ash (5.9-6.1%), nitrogen (8.6-8.8%), protein (41.0-42.1%), lipid (8.6-8.8%), carbohydrate (38.3-39.3%) and gross energy (22.2-22.3 MJÁkg −1 ) with highly similar fatty acid and amino acid profiles.CONCLUSIONS: This study demonstrates that the use of freshwater microalgae to remediate various aquaculture and agriculture wastewaters, while producing valuable nutrient-rich biomass, is feasible. We have found that a particular finfish aquaculture wastewater (CA2) contains all of the nutrients required for rapid microalgae growth, avoiding the need for expensive commercial fertilizers. The biochemical composition of C. sorokiniana grown on CA2 aquaculture wastewater has good potential for use as a nutrient-rich ingredient for animal and aquaculture feeds, fertilizers products, biofuels and other applications, resulting in remediated water that could be reused.

show abstract

Biochemical Characterization and In Vitro Digestibility of Protein Isolates from Hemp (Cannabis sativa L.) By-Products for Salmonid Feed Applications

Cited by 14 publications

References 39 publications

Nutritional Quality, Chemical, and Functional Characteristics of Hemp (Cannabis sativa ssp. sativa) Protein Isolate

Nutritional Quality, Chemical, and Functional Characteristics of Hemp (Cannabis sativa ssp. sativa) Protein Isolate

Hemp (<i>Cannabis sativa</i> L.) seeds nutritional aspects and food production perspectives: A review

Phycoremediation and simultaneous production of protein‐rich algal biomass from aquaculture and agriculture wastewaters

Contact Info

Product

Resources

About